In Vivo Rapid Investigation of CRISPR-Based Base Editing Components in <i>Escherichia coli</i> (IRI-CCE): A Platform for Evaluating Base Editing Tools and Their Components

In Vivo Rapid Investigation of CRISPR-Based Base Editing Components in <i>Escherichia coli</i> (IRI-CCE): A Platform for Evaluating Base Editing Tools and Their Components

Rapid assessment of clustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR/Cas)-based genome editing (GE) tools and their components is a critical aspect for successful GE applications in different organisms. In many bacteria, double-strand breaks (DSBs) generate...

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Bibliographic Details
Main Authors: Rahul Mahadev Shelake, Dibyajyoti Pramanik, Jae-Yean Kim
Format: Article
Language:English
Published: MDPI AG 2022-01-01
Series:International Journal of Molecular Sciences
Subjects:
base editing
CRISPR
genome editing
heterologous expression
Online Access:https://www.mdpi.com/1422-0067/23/3/1145

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