New mechanistic insights on Justicia vahlii Roth: UPLC-Q-TOF-MS and GC–MS based metabolomics, in-vivo, in-silico toxicological, antioxidant based anti-inflammatory and enzyme inhibition evaluation
Justicia vahlii Roth. (acanthaceae) is an important medicinal food plant used in pain relief and topical inflammation. The present study aimed to evaluate phytochemical composition, toxicity, anti-inflammatory, antioxidant and enzyme inhibition potential of n-butanol extract of J. vahlii (BEJv). The...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-10-01
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| Series: | Arabian Journal of Chemistry |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1878535222004518 |
| _version_ | 1811267198253006848 |
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| author | Abdul Basit Saeed Ahmad Kashif ur Rehman Khan Asmaa E. Sherif Hanan Y. Aati Chitchamai Ovatlarnporn Mohsin Abbas Khan Huma Rao Imtiaz Ahmad Muhammad Nadeem Shahzad Bilal Ahmad Ghalloo Hassan Shah Kifayat Ullah Khan Rizwana Dilshad |
| author_facet | Abdul Basit Saeed Ahmad Kashif ur Rehman Khan Asmaa E. Sherif Hanan Y. Aati Chitchamai Ovatlarnporn Mohsin Abbas Khan Huma Rao Imtiaz Ahmad Muhammad Nadeem Shahzad Bilal Ahmad Ghalloo Hassan Shah Kifayat Ullah Khan Rizwana Dilshad |
| author_sort | Abdul Basit |
| collection | DOAJ |
| description | Justicia vahlii Roth. (acanthaceae) is an important medicinal food plant used in pain relief and topical inflammation. The present study aimed to evaluate phytochemical composition, toxicity, anti-inflammatory, antioxidant and enzyme inhibition potential of n-butanol extract of J. vahlii (BEJv). The extract prepared through maceration was found rich in total phenolic contents (TPC) 196.08 ± 6.01 mg of Gallic acid equivalent (mg GAE/g DE) and total flavonoid contents (TFC) 59.08 ± 1.32 mg of Rutin equivalent (mg RE/g DE). The UPLC-Q-TOF-MS analysis of BEJv showed tentative identification of 87 compounds and 19 compounds were detected in GC–MS analysis. The HPLC-PDA quantification showed the presence of 14 polyphenols amongst which kaempferol (3.45 ± 0.21 µg/ mL DE) and ferulic acid (2.31 ± 1.30 µg/ mL DE) were found in highest quantity. The acute oral toxicity study revealed the safety and biocompatibility of the extract up to 3000 mg/kg in mice. There was no effect of BEJv on human normal liver cells (HL 7702) and very low cytotoxic effect on liver cancer cells (HepG2) and breast cancer cells (MCF-7). In anti-inflammatory evaluation, the BEJv treated groups showed significant inhibition (p < 0.001) of late phase carrageenan induced paw edema at 400 mg/kg and increased the levels of oxidative stress markers; catalase, superoxide dismutase (SOD) and glutathione (GSH) while decreased the inflammatory markers; interleukin-1beta (IL-β) and tumor necrosis factor alpha (TNF-α) in paw tissue of mice. BEJv displayed highest results in Ferric reducing antioxidant power (FRAP) assay 97. 21 ± 2.34 mg TE (trolox equivalent)/g DE, and highest activity 3.32 ± 0.31 mmol ACAE (acarbose equivalent)/g D.E against α-glucosidase. Docking study showed good docking score by the tested compounds against the various clinically significant enzymes. Conclusively the current study unveiled J. vahlii as novel non-toxic source with good antioxidant-mediated anti-inflammatory potential which strongly back the traditional use of the species in pain and inflammation. |
| first_indexed | 2024-04-12T20:58:05Z |
| format | Article |
| id | doaj.art-6913db9aebab43d48da2dc77fd7e1634 |
| institution | Directory Open Access Journal |
| issn | 1878-5352 |
| language | English |
| last_indexed | 2024-04-12T20:58:05Z |
| publishDate | 2022-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Arabian Journal of Chemistry |
| spelling | doaj.art-6913db9aebab43d48da2dc77fd7e16342022-12-22T03:16:56ZengElsevierArabian Journal of Chemistry1878-53522022-10-011510104135New mechanistic insights on Justicia vahlii Roth: UPLC-Q-TOF-MS and GC–MS based metabolomics, in-vivo, in-silico toxicological, antioxidant based anti-inflammatory and enzyme inhibition evaluationAbdul Basit0Saeed Ahmad1Kashif ur Rehman Khan2Asmaa E. Sherif3Hanan Y. Aati4Chitchamai Ovatlarnporn5Mohsin Abbas Khan6Huma Rao7Imtiaz Ahmad8Muhammad Nadeem Shahzad9Bilal Ahmad Ghalloo10Hassan Shah11Kifayat Ullah Khan12Rizwana Dilshad13Quaid-e-Azam College of Pharmacy, Quaid-e-Azam Educational Complex, Sahiwal, Punjab, PakistanDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, The Islamia University of Bahawalpur 63100, Punjab, PakistanDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, The Islamia University of Bahawalpur 63100, Punjab, Pakistan; Corresponding author.Department of Pharmacognosy, College of Pharmacy, Prince Sattam Bin Abdul Aziz University, Alkharj 11942, Saudi Arabia; Department of Pharmacognosy, Faculty of Pharmacy, Mansoura University, Mansoura 35516, EgyptDepartment of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh 11495, Saudi ArabiaDepartment of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat Yai 90112, Songkhla, ThailandDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, The Islamia University of Bahawalpur 63100, Punjab, PakistanDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, The Islamia University of Bahawalpur 63100, Punjab, PakistanDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, The Islamia University of Bahawalpur 63100, Punjab, PakistanDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, The Islamia University of Bahawalpur 63100, Punjab, PakistanDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, The Islamia University of Bahawalpur 63100, Punjab, PakistanDepartment of Pharmaceutics, Faculty of Pharmacy, The Islamia University of Bahawalpur 63100, Punjab, PakistanQuaid-e-Azam College of Pharmacy, Quaid-e-Azam Educational Complex, Sahiwal, Punjab, PakistanDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, The Islamia University of Bahawalpur 63100, Punjab, PakistanJusticia vahlii Roth. (acanthaceae) is an important medicinal food plant used in pain relief and topical inflammation. The present study aimed to evaluate phytochemical composition, toxicity, anti-inflammatory, antioxidant and enzyme inhibition potential of n-butanol extract of J. vahlii (BEJv). The extract prepared through maceration was found rich in total phenolic contents (TPC) 196.08 ± 6.01 mg of Gallic acid equivalent (mg GAE/g DE) and total flavonoid contents (TFC) 59.08 ± 1.32 mg of Rutin equivalent (mg RE/g DE). The UPLC-Q-TOF-MS analysis of BEJv showed tentative identification of 87 compounds and 19 compounds were detected in GC–MS analysis. The HPLC-PDA quantification showed the presence of 14 polyphenols amongst which kaempferol (3.45 ± 0.21 µg/ mL DE) and ferulic acid (2.31 ± 1.30 µg/ mL DE) were found in highest quantity. The acute oral toxicity study revealed the safety and biocompatibility of the extract up to 3000 mg/kg in mice. There was no effect of BEJv on human normal liver cells (HL 7702) and very low cytotoxic effect on liver cancer cells (HepG2) and breast cancer cells (MCF-7). In anti-inflammatory evaluation, the BEJv treated groups showed significant inhibition (p < 0.001) of late phase carrageenan induced paw edema at 400 mg/kg and increased the levels of oxidative stress markers; catalase, superoxide dismutase (SOD) and glutathione (GSH) while decreased the inflammatory markers; interleukin-1beta (IL-β) and tumor necrosis factor alpha (TNF-α) in paw tissue of mice. BEJv displayed highest results in Ferric reducing antioxidant power (FRAP) assay 97. 21 ± 2.34 mg TE (trolox equivalent)/g DE, and highest activity 3.32 ± 0.31 mmol ACAE (acarbose equivalent)/g D.E against α-glucosidase. Docking study showed good docking score by the tested compounds against the various clinically significant enzymes. Conclusively the current study unveiled J. vahlii as novel non-toxic source with good antioxidant-mediated anti-inflammatory potential which strongly back the traditional use of the species in pain and inflammation.http://www.sciencedirect.com/science/article/pii/S1878535222004518Justicia vahliiChemical profilingAnti-inflammatoryOxidative stressEnzyme inhibition |
| spellingShingle | Abdul Basit Saeed Ahmad Kashif ur Rehman Khan Asmaa E. Sherif Hanan Y. Aati Chitchamai Ovatlarnporn Mohsin Abbas Khan Huma Rao Imtiaz Ahmad Muhammad Nadeem Shahzad Bilal Ahmad Ghalloo Hassan Shah Kifayat Ullah Khan Rizwana Dilshad New mechanistic insights on Justicia vahlii Roth: UPLC-Q-TOF-MS and GC–MS based metabolomics, in-vivo, in-silico toxicological, antioxidant based anti-inflammatory and enzyme inhibition evaluation Arabian Journal of Chemistry Justicia vahlii Chemical profiling Anti-inflammatory Oxidative stress Enzyme inhibition |
| title | New mechanistic insights on Justicia vahlii Roth: UPLC-Q-TOF-MS and GC–MS based metabolomics, in-vivo, in-silico toxicological, antioxidant based anti-inflammatory and enzyme inhibition evaluation |
| title_full | New mechanistic insights on Justicia vahlii Roth: UPLC-Q-TOF-MS and GC–MS based metabolomics, in-vivo, in-silico toxicological, antioxidant based anti-inflammatory and enzyme inhibition evaluation |
| title_fullStr | New mechanistic insights on Justicia vahlii Roth: UPLC-Q-TOF-MS and GC–MS based metabolomics, in-vivo, in-silico toxicological, antioxidant based anti-inflammatory and enzyme inhibition evaluation |
| title_full_unstemmed | New mechanistic insights on Justicia vahlii Roth: UPLC-Q-TOF-MS and GC–MS based metabolomics, in-vivo, in-silico toxicological, antioxidant based anti-inflammatory and enzyme inhibition evaluation |
| title_short | New mechanistic insights on Justicia vahlii Roth: UPLC-Q-TOF-MS and GC–MS based metabolomics, in-vivo, in-silico toxicological, antioxidant based anti-inflammatory and enzyme inhibition evaluation |
| title_sort | new mechanistic insights on justicia vahlii roth uplc q tof ms and gc ms based metabolomics in vivo in silico toxicological antioxidant based anti inflammatory and enzyme inhibition evaluation |
| topic | Justicia vahlii Chemical profiling Anti-inflammatory Oxidative stress Enzyme inhibition |
| url | http://www.sciencedirect.com/science/article/pii/S1878535222004518 |
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