Characterization of the porcine <it>CDKN3 </it>gene as a potential candidate for congenital splay leg in piglets
<p>Abstract</p> <p>Congenital splay leg is a hereditary disease observed in newborn piglets. The etiology and pathogenetic mechanism of the disorder are still unknown. The gene for cyclin-dependent protein kinase inhibitor 3 (<it>CDKN3</it>) was identified as a potentia...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | deu |
| Published: |
BMC
2003-06-01
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| Series: | Genetics Selection Evolution |
| Subjects: | |
| Online Access: | http://www.gsejournal.org/content/35/S1/S157 |
| _version_ | 1818752150637903872 |
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| author | Maak Steffen Jaesert Simone Neumann Karsten von Lengerken Gerhard |
| author_facet | Maak Steffen Jaesert Simone Neumann Karsten von Lengerken Gerhard |
| author_sort | Maak Steffen |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Congenital splay leg is a hereditary disease observed in newborn piglets. The etiology and pathogenetic mechanism of the disorder are still unknown. The gene for cyclin-dependent protein kinase inhibitor 3 (<it>CDKN3</it>) was identified as a potential candidate gene in a differential display experiment. We analyzed the gene on sequence variations and compared its expression in <it>M. biceps femoris </it>between healthy and affected piglets. Comparative sequencing of the coding region of three healthy and four splay leg piglets revealed twelve single nucleotide polymorphisms (SNP) resulting in six amino acid exchanges in the deduced sequences. However, all polymorphisms were observed in healthy as well as in splay leg piglets thus excluding structural differences of the gene as a cause of the disease. Besides full length transcripts, we found a variety of aberrantly transcribed cDNA in clones derived from <it>M. biceps femoris </it>of healthy as well as of splay leg piglets. All alternative transcripts coexist with normal cDNA. Expression analysis revealed a trend towards higher values in <it>M. biceps femoris </it>of splay leg piglets supporting the results obtained from a differential display.</p> |
| first_indexed | 2024-12-18T04:46:53Z |
| format | Article |
| id | doaj.art-6915fd0bffb04044a9f57e934801585b |
| institution | Directory Open Access Journal |
| issn | 0999-193X 1297-9686 |
| language | deu |
| last_indexed | 2024-12-18T04:46:53Z |
| publishDate | 2003-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Genetics Selection Evolution |
| spelling | doaj.art-6915fd0bffb04044a9f57e934801585b2022-12-21T21:20:33ZdeuBMCGenetics Selection Evolution0999-193X1297-96862003-06-0135Suppl 1S157S16510.1186/1297-9686-35-S1-S157Characterization of the porcine <it>CDKN3 </it>gene as a potential candidate for congenital splay leg in pigletsMaak SteffenJaesert SimoneNeumann Karstenvon Lengerken Gerhard<p>Abstract</p> <p>Congenital splay leg is a hereditary disease observed in newborn piglets. The etiology and pathogenetic mechanism of the disorder are still unknown. The gene for cyclin-dependent protein kinase inhibitor 3 (<it>CDKN3</it>) was identified as a potential candidate gene in a differential display experiment. We analyzed the gene on sequence variations and compared its expression in <it>M. biceps femoris </it>between healthy and affected piglets. Comparative sequencing of the coding region of three healthy and four splay leg piglets revealed twelve single nucleotide polymorphisms (SNP) resulting in six amino acid exchanges in the deduced sequences. However, all polymorphisms were observed in healthy as well as in splay leg piglets thus excluding structural differences of the gene as a cause of the disease. Besides full length transcripts, we found a variety of aberrantly transcribed cDNA in clones derived from <it>M. biceps femoris </it>of healthy as well as of splay leg piglets. All alternative transcripts coexist with normal cDNA. Expression analysis revealed a trend towards higher values in <it>M. biceps femoris </it>of splay leg piglets supporting the results obtained from a differential display.</p>http://www.gsejournal.org/content/35/S1/S157CDKN3 geneexpression analysiscongenital splay legpiglet |
| spellingShingle | Maak Steffen Jaesert Simone Neumann Karsten von Lengerken Gerhard Characterization of the porcine <it>CDKN3 </it>gene as a potential candidate for congenital splay leg in piglets Genetics Selection Evolution CDKN3 gene expression analysis congenital splay leg piglet |
| title | Characterization of the porcine <it>CDKN3 </it>gene as a potential candidate for congenital splay leg in piglets |
| title_full | Characterization of the porcine <it>CDKN3 </it>gene as a potential candidate for congenital splay leg in piglets |
| title_fullStr | Characterization of the porcine <it>CDKN3 </it>gene as a potential candidate for congenital splay leg in piglets |
| title_full_unstemmed | Characterization of the porcine <it>CDKN3 </it>gene as a potential candidate for congenital splay leg in piglets |
| title_short | Characterization of the porcine <it>CDKN3 </it>gene as a potential candidate for congenital splay leg in piglets |
| title_sort | characterization of the porcine it cdkn3 it gene as a potential candidate for congenital splay leg in piglets |
| topic | CDKN3 gene expression analysis congenital splay leg piglet |
| url | http://www.gsejournal.org/content/35/S1/S157 |
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