Enhanced Resistance to Fungal and Bacterial Diseases Due to Overexpression of BSR1, a Rice RLCK, in Sugarcane, Tomato, and Torenia
Sugarcane smut caused by <i>Sporisorium scitamineum</i> is one of the most devastating sugarcane diseases. Furthermore, <i>Rhizoctonia solani</i> causes severe diseases in various crops including rice, tomato, potato, sugar beet, tobacco, and torenia. However, effective disea...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-02-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/4/3644 |
| Summary: | Sugarcane smut caused by <i>Sporisorium scitamineum</i> is one of the most devastating sugarcane diseases. Furthermore, <i>Rhizoctonia solani</i> causes severe diseases in various crops including rice, tomato, potato, sugar beet, tobacco, and torenia. However, effective disease-resistant genes against these pathogens have not been identified in target crops. Therefore, the transgenic approach can be used since conventional cross-breeding is not applicable. Herein, the overexpression of <i>BROAD-SPECTRUM RESISTANCE 1</i> (<i>BSR1</i>), a rice receptor-like cytoplasmic kinase, was conducted in sugarcane, tomato and torenia. <i>BSR1</i>-overexpressing tomatoes exhibited resistance to the bacteria <i>Pseudomonas syringae</i> pv. <i>tomato</i> DC3000 and the fungus <i>R. solani,</i> whereas <i>BSR1</i>-overexpressing torenia showed resistance to <i>R. solani</i> in the growth room. Additionally, <i>BSR1</i> overexpression conferred resistance to sugarcane smut in the greenhouse. These three <i>BSR1</i>-overexpressing crops exhibited normal growth and morphologies except in the case of exceedingly high levels of overexpression. These results indicate that <i>BSR1</i> overexpression is a simple and effective tool for conferring broad-spectrum disease resistance to many crops. |
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| ISSN: | 1661-6596 1422-0067 |