Enhanced Resistance to Fungal and Bacterial Diseases Due to Overexpression of BSR1, a Rice RLCK, in Sugarcane, Tomato, and Torenia
Sugarcane smut caused by <i>Sporisorium scitamineum</i> is one of the most devastating sugarcane diseases. Furthermore, <i>Rhizoctonia solani</i> causes severe diseases in various crops including rice, tomato, potato, sugar beet, tobacco, and torenia. However, effective disea...
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MDPI AG
2023-02-01
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| Series: | International Journal of Molecular Sciences |
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| author | Satoru Maeda Wataru Ackley Naoki Yokotani Katsutomo Sasaki Norihiro Ohtsubo Kenji Oda Masaki Mori |
| author_facet | Satoru Maeda Wataru Ackley Naoki Yokotani Katsutomo Sasaki Norihiro Ohtsubo Kenji Oda Masaki Mori |
| author_sort | Satoru Maeda |
| collection | DOAJ |
| description | Sugarcane smut caused by <i>Sporisorium scitamineum</i> is one of the most devastating sugarcane diseases. Furthermore, <i>Rhizoctonia solani</i> causes severe diseases in various crops including rice, tomato, potato, sugar beet, tobacco, and torenia. However, effective disease-resistant genes against these pathogens have not been identified in target crops. Therefore, the transgenic approach can be used since conventional cross-breeding is not applicable. Herein, the overexpression of <i>BROAD-SPECTRUM RESISTANCE 1</i> (<i>BSR1</i>), a rice receptor-like cytoplasmic kinase, was conducted in sugarcane, tomato and torenia. <i>BSR1</i>-overexpressing tomatoes exhibited resistance to the bacteria <i>Pseudomonas syringae</i> pv. <i>tomato</i> DC3000 and the fungus <i>R. solani,</i> whereas <i>BSR1</i>-overexpressing torenia showed resistance to <i>R. solani</i> in the growth room. Additionally, <i>BSR1</i> overexpression conferred resistance to sugarcane smut in the greenhouse. These three <i>BSR1</i>-overexpressing crops exhibited normal growth and morphologies except in the case of exceedingly high levels of overexpression. These results indicate that <i>BSR1</i> overexpression is a simple and effective tool for conferring broad-spectrum disease resistance to many crops. |
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| spelling | doaj.art-691be836c49542bea4c5617389e4ee352023-11-16T21:02:55ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-02-01244364410.3390/ijms24043644Enhanced Resistance to Fungal and Bacterial Diseases Due to Overexpression of BSR1, a Rice RLCK, in Sugarcane, Tomato, and ToreniaSatoru Maeda0Wataru Ackley1Naoki Yokotani2Katsutomo Sasaki3Norihiro Ohtsubo4Kenji Oda5Masaki Mori6Institute of Agrobiological Sciences, NARO (NIAS), Tsukuba 305-8634, JapanInstitute of Livestock and Grassland Science, NARO (NILGS), Nasushiobara 329-2793, JapanResearch Institute for Biological Sciences, Okayama Prefectural Technology Center for Agriculture, Forestry, and Fisheries, Okayama 716-1241, JapanInstitute of Vegetable and Floriculture Science, NARO (NIVFS), Tsukuba 305-0852, JapanInstitute of Vegetable and Floriculture Science, NARO (NIVFS), Tsukuba 305-0852, JapanResearch Institute for Biological Sciences, Okayama Prefectural Technology Center for Agriculture, Forestry, and Fisheries, Okayama 716-1241, JapanInstitute of Agrobiological Sciences, NARO (NIAS), Tsukuba 305-8634, JapanSugarcane smut caused by <i>Sporisorium scitamineum</i> is one of the most devastating sugarcane diseases. Furthermore, <i>Rhizoctonia solani</i> causes severe diseases in various crops including rice, tomato, potato, sugar beet, tobacco, and torenia. However, effective disease-resistant genes against these pathogens have not been identified in target crops. Therefore, the transgenic approach can be used since conventional cross-breeding is not applicable. Herein, the overexpression of <i>BROAD-SPECTRUM RESISTANCE 1</i> (<i>BSR1</i>), a rice receptor-like cytoplasmic kinase, was conducted in sugarcane, tomato and torenia. <i>BSR1</i>-overexpressing tomatoes exhibited resistance to the bacteria <i>Pseudomonas syringae</i> pv. <i>tomato</i> DC3000 and the fungus <i>R. solani,</i> whereas <i>BSR1</i>-overexpressing torenia showed resistance to <i>R. solani</i> in the growth room. Additionally, <i>BSR1</i> overexpression conferred resistance to sugarcane smut in the greenhouse. These three <i>BSR1</i>-overexpressing crops exhibited normal growth and morphologies except in the case of exceedingly high levels of overexpression. These results indicate that <i>BSR1</i> overexpression is a simple and effective tool for conferring broad-spectrum disease resistance to many crops.https://www.mdpi.com/1422-0067/24/4/3644<i>BSR1</i>RLCK (receptor-like cytoplasmic kinase)disease resistance<i>Sporisorium scitamineum</i><i>Rhizoctonia solani</i><i>Pseudomonas syringae</i> |
| spellingShingle | Satoru Maeda Wataru Ackley Naoki Yokotani Katsutomo Sasaki Norihiro Ohtsubo Kenji Oda Masaki Mori Enhanced Resistance to Fungal and Bacterial Diseases Due to Overexpression of BSR1, a Rice RLCK, in Sugarcane, Tomato, and Torenia International Journal of Molecular Sciences <i>BSR1</i> RLCK (receptor-like cytoplasmic kinase) disease resistance <i>Sporisorium scitamineum</i> <i>Rhizoctonia solani</i> <i>Pseudomonas syringae</i> |
| title | Enhanced Resistance to Fungal and Bacterial Diseases Due to Overexpression of BSR1, a Rice RLCK, in Sugarcane, Tomato, and Torenia |
| title_full | Enhanced Resistance to Fungal and Bacterial Diseases Due to Overexpression of BSR1, a Rice RLCK, in Sugarcane, Tomato, and Torenia |
| title_fullStr | Enhanced Resistance to Fungal and Bacterial Diseases Due to Overexpression of BSR1, a Rice RLCK, in Sugarcane, Tomato, and Torenia |
| title_full_unstemmed | Enhanced Resistance to Fungal and Bacterial Diseases Due to Overexpression of BSR1, a Rice RLCK, in Sugarcane, Tomato, and Torenia |
| title_short | Enhanced Resistance to Fungal and Bacterial Diseases Due to Overexpression of BSR1, a Rice RLCK, in Sugarcane, Tomato, and Torenia |
| title_sort | enhanced resistance to fungal and bacterial diseases due to overexpression of bsr1 a rice rlck in sugarcane tomato and torenia |
| topic | <i>BSR1</i> RLCK (receptor-like cytoplasmic kinase) disease resistance <i>Sporisorium scitamineum</i> <i>Rhizoctonia solani</i> <i>Pseudomonas syringae</i> |
| url | https://www.mdpi.com/1422-0067/24/4/3644 |
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