Generation of two human ISG15 knockout iPSC clones using CRISPR/Cas9 editing
Interferon stimulated gene 15 (ISG15) is one of the most highly upregulated proteins in response to viral infection and is involved in numerous pathways with multiple mechanisms of actions. ISG15 deficiency has been reported to induce type I interferonopathy owing to defective negative regulation of...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-01-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506120304360 |
| _version_ | 1818557224718434304 |
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| author | S. Merkert M.-C. Jaboreck L. Engels M.N.H. Malik G. Göhring F. Pessler U. Martin R. Olmer |
| author_facet | S. Merkert M.-C. Jaboreck L. Engels M.N.H. Malik G. Göhring F. Pessler U. Martin R. Olmer |
| author_sort | S. Merkert |
| collection | DOAJ |
| description | Interferon stimulated gene 15 (ISG15) is one of the most highly upregulated proteins in response to viral infection and is involved in numerous pathways with multiple mechanisms of actions. ISG15 deficiency has been reported to induce type I interferonopathy owing to defective negative regulation of IFN-I signalling as well as enhanced antiviral protection. Here, we have generated ISG15 knockout clones from human iPSCs, which provide useful cell resources to study mechanisms of ISG15 deficiency and gain more insight into the biological function of ISG15. |
| first_indexed | 2024-12-13T23:57:02Z |
| format | Article |
| id | doaj.art-691d2f4eb77f4f1ca4437b3f5ce3f51c |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-12-13T23:57:02Z |
| publishDate | 2021-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-691d2f4eb77f4f1ca4437b3f5ce3f51c2022-12-21T23:26:30ZengElsevierStem Cell Research1873-50612021-01-0150102135Generation of two human ISG15 knockout iPSC clones using CRISPR/Cas9 editingS. Merkert0M.-C. Jaboreck1L. Engels2M.N.H. Malik3G. Göhring4F. Pessler5U. Martin6R. Olmer7Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiothoracic, Transplantation and Vascular Surgery (HTTG), REBIRTH-Research Center for Translational and Regenerative Medicine, Hannover Medical School, 30625 Hannover, Germany; Biomedical Research in Endstage and Obstructive Lung Disease (BREATH), Member of the German Center for Lung Research (DZL), Germany; Corresponding author.Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiothoracic, Transplantation and Vascular Surgery (HTTG), REBIRTH-Research Center for Translational and Regenerative Medicine, Hannover Medical School, 30625 Hannover, Germany; Biomedical Research in Endstage and Obstructive Lung Disease (BREATH), Member of the German Center for Lung Research (DZL), GermanyLeibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiothoracic, Transplantation and Vascular Surgery (HTTG), REBIRTH-Research Center for Translational and Regenerative Medicine, Hannover Medical School, 30625 Hannover, Germany; Biomedical Research in Endstage and Obstructive Lung Disease (BREATH), Member of the German Center for Lung Research (DZL), GermanyTWINCORE Centre for Experimental and Clinical Infection Research, Centre for Individualized Infection Medicine, 30625 Hannover, Germany; Helmholtz-Centre for Infection Research Braunschweig, 38124 Braunschweig, GermanyDepartment of Human Genetics, Hannover Medical School, 30625 Hannover, GermanyTWINCORE Centre for Experimental and Clinical Infection Research, Centre for Individualized Infection Medicine, 30625 Hannover, Germany; Helmholtz-Centre for Infection Research Braunschweig, 38124 Braunschweig, GermanyLeibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiothoracic, Transplantation and Vascular Surgery (HTTG), REBIRTH-Research Center for Translational and Regenerative Medicine, Hannover Medical School, 30625 Hannover, Germany; Biomedical Research in Endstage and Obstructive Lung Disease (BREATH), Member of the German Center for Lung Research (DZL), GermanyLeibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiothoracic, Transplantation and Vascular Surgery (HTTG), REBIRTH-Research Center for Translational and Regenerative Medicine, Hannover Medical School, 30625 Hannover, Germany; Biomedical Research in Endstage and Obstructive Lung Disease (BREATH), Member of the German Center for Lung Research (DZL), GermanyInterferon stimulated gene 15 (ISG15) is one of the most highly upregulated proteins in response to viral infection and is involved in numerous pathways with multiple mechanisms of actions. ISG15 deficiency has been reported to induce type I interferonopathy owing to defective negative regulation of IFN-I signalling as well as enhanced antiviral protection. Here, we have generated ISG15 knockout clones from human iPSCs, which provide useful cell resources to study mechanisms of ISG15 deficiency and gain more insight into the biological function of ISG15.http://www.sciencedirect.com/science/article/pii/S1873506120304360 |
| spellingShingle | S. Merkert M.-C. Jaboreck L. Engels M.N.H. Malik G. Göhring F. Pessler U. Martin R. Olmer Generation of two human ISG15 knockout iPSC clones using CRISPR/Cas9 editing Stem Cell Research |
| title | Generation of two human ISG15 knockout iPSC clones using CRISPR/Cas9 editing |
| title_full | Generation of two human ISG15 knockout iPSC clones using CRISPR/Cas9 editing |
| title_fullStr | Generation of two human ISG15 knockout iPSC clones using CRISPR/Cas9 editing |
| title_full_unstemmed | Generation of two human ISG15 knockout iPSC clones using CRISPR/Cas9 editing |
| title_short | Generation of two human ISG15 knockout iPSC clones using CRISPR/Cas9 editing |
| title_sort | generation of two human isg15 knockout ipsc clones using crispr cas9 editing |
| url | http://www.sciencedirect.com/science/article/pii/S1873506120304360 |
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