Extremely divergent COI sequences within an amphipod species complex: A possible role for endosymbionts?
Abstract Some heritable endosymbionts can affect host mtDNA evolution in various ways. Amphipods host diverse endosymbionts, but whether their mtDNA has been influenced by these endosymbionts has yet to be considered. Here, we investigated the role of endosymbionts (microsporidians and Rickettsia) i...
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| Format: | Article |
| Language: | English |
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Wiley
2022-10-01
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| Series: | Ecology and Evolution |
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| Online Access: | https://doi.org/10.1002/ece3.9448 |
| _version_ | 1828722536788000768 |
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| author | Eunji Park Robert Poulin |
| author_facet | Eunji Park Robert Poulin |
| author_sort | Eunji Park |
| collection | DOAJ |
| description | Abstract Some heritable endosymbionts can affect host mtDNA evolution in various ways. Amphipods host diverse endosymbionts, but whether their mtDNA has been influenced by these endosymbionts has yet to be considered. Here, we investigated the role of endosymbionts (microsporidians and Rickettsia) in explaining highly divergent COI sequences in Paracalliope fluviatilis species complex, the most common freshwater amphipods in New Zealand. We first contrasted phylogeographic patterns using COI, ITS, and 28S sequences. While molecular species delimitation methods based on 28S sequences supported 3–4 potential species (N, C, SA, and SB) among freshwater lineages, COI sequences supported 17–27 putative species reflecting high inter‐population divergence. The deep divergence between NC and S lineages (~20%; 28S) and the substitution saturation on the 3rd codon position of COI detected even within one lineage (SA) indicate a very high level of morphological stasis. Interestingly, individuals infected and uninfected by Rickettsia comprised divergent COI lineages in one of four populations tested, suggesting a potential influence of endosymbionts in mtDNA patterns. We propose several plausible explanations for divergent COI lineages, although they would need further testing with multiple lines of evidence. Lastly, due to common morphological stasis and the presence of endosymbionts, phylogeographic patterns of amphipods based on mtDNA should be interpreted with caution. |
| first_indexed | 2024-04-12T05:41:08Z |
| format | Article |
| id | doaj.art-691fdd458d6d4b27b09f7c203c0fcf9c |
| institution | Directory Open Access Journal |
| issn | 2045-7758 |
| language | English |
| last_indexed | 2024-04-12T05:41:08Z |
| publishDate | 2022-10-01 |
| publisher | Wiley |
| record_format | Article |
| series | Ecology and Evolution |
| spelling | doaj.art-691fdd458d6d4b27b09f7c203c0fcf9c2022-12-22T03:45:39ZengWileyEcology and Evolution2045-77582022-10-011210n/an/a10.1002/ece3.9448Extremely divergent COI sequences within an amphipod species complex: A possible role for endosymbionts?Eunji Park0Robert Poulin1Department of Zoology University of Otago Dunedin New ZealandDepartment of Zoology University of Otago Dunedin New ZealandAbstract Some heritable endosymbionts can affect host mtDNA evolution in various ways. Amphipods host diverse endosymbionts, but whether their mtDNA has been influenced by these endosymbionts has yet to be considered. Here, we investigated the role of endosymbionts (microsporidians and Rickettsia) in explaining highly divergent COI sequences in Paracalliope fluviatilis species complex, the most common freshwater amphipods in New Zealand. We first contrasted phylogeographic patterns using COI, ITS, and 28S sequences. While molecular species delimitation methods based on 28S sequences supported 3–4 potential species (N, C, SA, and SB) among freshwater lineages, COI sequences supported 17–27 putative species reflecting high inter‐population divergence. The deep divergence between NC and S lineages (~20%; 28S) and the substitution saturation on the 3rd codon position of COI detected even within one lineage (SA) indicate a very high level of morphological stasis. Interestingly, individuals infected and uninfected by Rickettsia comprised divergent COI lineages in one of four populations tested, suggesting a potential influence of endosymbionts in mtDNA patterns. We propose several plausible explanations for divergent COI lineages, although they would need further testing with multiple lines of evidence. Lastly, due to common morphological stasis and the presence of endosymbionts, phylogeographic patterns of amphipods based on mtDNA should be interpreted with caution.https://doi.org/10.1002/ece3.9448cryptic speciesintracellular parasitesmorphological conservatismspecies delimitationsubstitution saturation |
| spellingShingle | Eunji Park Robert Poulin Extremely divergent COI sequences within an amphipod species complex: A possible role for endosymbionts? Ecology and Evolution cryptic species intracellular parasites morphological conservatism species delimitation substitution saturation |
| title | Extremely divergent COI sequences within an amphipod species complex: A possible role for endosymbionts? |
| title_full | Extremely divergent COI sequences within an amphipod species complex: A possible role for endosymbionts? |
| title_fullStr | Extremely divergent COI sequences within an amphipod species complex: A possible role for endosymbionts? |
| title_full_unstemmed | Extremely divergent COI sequences within an amphipod species complex: A possible role for endosymbionts? |
| title_short | Extremely divergent COI sequences within an amphipod species complex: A possible role for endosymbionts? |
| title_sort | extremely divergent coi sequences within an amphipod species complex a possible role for endosymbionts |
| topic | cryptic species intracellular parasites morphological conservatism species delimitation substitution saturation |
| url | https://doi.org/10.1002/ece3.9448 |
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