Facilitation of L-type Ca2+ Currents by Fluid Flow in Rabbit Cerebral Artery Myocytes
Blood vessels are receptive to hemodynamic forces, such as blood pressure and flow, which result in myogenic responses. The present study aimed to investigate the effect of mechanical stresses on L-type voltage-dependent Ca2+ channels in rabbit cerebral artery myocytes. Cell swelling induced by the...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2005-01-01
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| Series: | Journal of Pharmacological Sciences |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1347861319321590 |
| _version_ | 1818326570175037440 |
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| author | Satoshi Amano Tomohisa Ishikawa Koichi Nakayama |
| author_facet | Satoshi Amano Tomohisa Ishikawa Koichi Nakayama |
| author_sort | Satoshi Amano |
| collection | DOAJ |
| description | Blood vessels are receptive to hemodynamic forces, such as blood pressure and flow, which result in myogenic responses. The present study aimed to investigate the effect of mechanical stresses on L-type voltage-dependent Ca2+ channels in rabbit cerebral artery myocytes. Cell swelling induced by the exposure to a 16% hypotonic solution increased peak values of whole-cell Ba2+ currents (IBa). Similarly, an elevation of bath perfusion rate increased peak values of IBa. However, the response was reduced by the continued fluid flow stimulation and the current amplitude almost returned to the baseline. This reduction of the current was abolished by pretreatment with thapsigargin, implying the contribution of Ca2+ release from the sarcoplasmic reticulum to the response. These results suggest that L-type Ca2+ currents are facilitated not only by cell swelling but also by fluid flow in cerebral artery myocytes. Keywords:: L-type voltage-dependent Ca2+ channel, rabbit cerebral artery, patch clamp, swelling, flow |
| first_indexed | 2024-12-13T12:02:28Z |
| format | Article |
| id | doaj.art-6922ac50a1a4433e8abd8b97aaa0a1e9 |
| institution | Directory Open Access Journal |
| issn | 1347-8613 |
| language | English |
| last_indexed | 2024-12-13T12:02:28Z |
| publishDate | 2005-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Pharmacological Sciences |
| spelling | doaj.art-6922ac50a1a4433e8abd8b97aaa0a1e92022-12-21T23:47:04ZengElsevierJournal of Pharmacological Sciences1347-86132005-01-01984425429Facilitation of L-type Ca2+ Currents by Fluid Flow in Rabbit Cerebral Artery MyocytesSatoshi Amano0Tomohisa Ishikawa1Koichi Nakayama2Department of Cellular and Molecular Pharmacology, Graduate School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka City, Shizuoka 422-8526, JapanDepartment of Cellular and Molecular Pharmacology, Graduate School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka City, Shizuoka 422-8526, Japan; Corresponding author. FAX: +81-54-264-5696 E-mail: ishikat@u-shizuoka-ken.ac.jpDepartment of Cellular and Molecular Pharmacology, Graduate School of Pharmaceutical Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka City, Shizuoka 422-8526, JapanBlood vessels are receptive to hemodynamic forces, such as blood pressure and flow, which result in myogenic responses. The present study aimed to investigate the effect of mechanical stresses on L-type voltage-dependent Ca2+ channels in rabbit cerebral artery myocytes. Cell swelling induced by the exposure to a 16% hypotonic solution increased peak values of whole-cell Ba2+ currents (IBa). Similarly, an elevation of bath perfusion rate increased peak values of IBa. However, the response was reduced by the continued fluid flow stimulation and the current amplitude almost returned to the baseline. This reduction of the current was abolished by pretreatment with thapsigargin, implying the contribution of Ca2+ release from the sarcoplasmic reticulum to the response. These results suggest that L-type Ca2+ currents are facilitated not only by cell swelling but also by fluid flow in cerebral artery myocytes. Keywords:: L-type voltage-dependent Ca2+ channel, rabbit cerebral artery, patch clamp, swelling, flowhttp://www.sciencedirect.com/science/article/pii/S1347861319321590 |
| spellingShingle | Satoshi Amano Tomohisa Ishikawa Koichi Nakayama Facilitation of L-type Ca2+ Currents by Fluid Flow in Rabbit Cerebral Artery Myocytes Journal of Pharmacological Sciences |
| title | Facilitation of L-type Ca2+ Currents by Fluid Flow in Rabbit Cerebral Artery Myocytes |
| title_full | Facilitation of L-type Ca2+ Currents by Fluid Flow in Rabbit Cerebral Artery Myocytes |
| title_fullStr | Facilitation of L-type Ca2+ Currents by Fluid Flow in Rabbit Cerebral Artery Myocytes |
| title_full_unstemmed | Facilitation of L-type Ca2+ Currents by Fluid Flow in Rabbit Cerebral Artery Myocytes |
| title_short | Facilitation of L-type Ca2+ Currents by Fluid Flow in Rabbit Cerebral Artery Myocytes |
| title_sort | facilitation of l type ca2 currents by fluid flow in rabbit cerebral artery myocytes |
| url | http://www.sciencedirect.com/science/article/pii/S1347861319321590 |
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