Surface Plasmon Resonance Based on Molecularly Imprinted Polymeric Film for <span style="font-variant: small-caps">l</span>-Phenylalanine Detection
In this study, we designed a simple, rapid, sensitive and selective surface plasmon resonance (SPR) sensor for detection of L-phenylalaine by utilizing molecular imprinting technology. <span style="font-variant: small-caps;">l</span>-phenylalanine imprinted and non-imprinted po...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-01-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/11/1/21 |
| _version_ | 1797410895552315392 |
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| author | Duygu Çimen Nilay Bereli Adil Denizli |
| author_facet | Duygu Çimen Nilay Bereli Adil Denizli |
| author_sort | Duygu Çimen |
| collection | DOAJ |
| description | In this study, we designed a simple, rapid, sensitive and selective surface plasmon resonance (SPR) sensor for detection of L-phenylalaine by utilizing molecular imprinting technology. <span style="font-variant: small-caps;">l</span>-phenylalanine imprinted and non-imprinted poly(2-hydroxyethyl methacrylate-methacryloyl-<span style="font-variant: small-caps;">l</span>-phenylalanine) polymeric films were synthesized onto SPR chip surfaces using ultraviolet polymerization. <span style="font-variant: small-caps;">l</span>-phenyalanine imprinted and non-imprinted SPR sensors were characterized by using contact angle, atomic force microscopy and ellipsometry. After characterization studies, kinetic studies were carried out in the concentration range of 5.0–400.0 μM. The limit of detection and quantification were obtained as 0.0085 and 0.0285 μM, respectively. The response time for the test including equilibration, adsorption and desorption was approximately 9 min. The selectivity studies of the <span style="font-variant: small-caps;">l</span>-phenylalanine imprinted SPR sensor was performed in the presence of <span style="font-variant: small-caps;">d</span>-phenylalanine and <span style="font-variant: small-caps;">l</span>-tryptophan. Validation studies were carried out via enzyme-linked immunosorbent analysis technique in order to demonstrate the applicability and superiority of the <span style="font-variant: small-caps;">l</span>-phenylalanine imprinted SPR sensor. |
| first_indexed | 2024-03-09T04:36:52Z |
| format | Article |
| id | doaj.art-6926778b094a482281d90900a68e9cb5 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-09T04:36:52Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-6926778b094a482281d90900a68e9cb52023-12-03T13:26:18ZengMDPI AGBiosensors2079-63742021-01-011112110.3390/bios11010021Surface Plasmon Resonance Based on Molecularly Imprinted Polymeric Film for <span style="font-variant: small-caps">l</span>-Phenylalanine DetectionDuygu Çimen0Nilay Bereli1Adil Denizli2Department of Chemistry, Hacettepe University, Beytepe, 06800 Ankara, TurkeyDepartment of Chemistry, Hacettepe University, Beytepe, 06800 Ankara, TurkeyDepartment of Chemistry, Hacettepe University, Beytepe, 06800 Ankara, TurkeyIn this study, we designed a simple, rapid, sensitive and selective surface plasmon resonance (SPR) sensor for detection of L-phenylalaine by utilizing molecular imprinting technology. <span style="font-variant: small-caps;">l</span>-phenylalanine imprinted and non-imprinted poly(2-hydroxyethyl methacrylate-methacryloyl-<span style="font-variant: small-caps;">l</span>-phenylalanine) polymeric films were synthesized onto SPR chip surfaces using ultraviolet polymerization. <span style="font-variant: small-caps;">l</span>-phenyalanine imprinted and non-imprinted SPR sensors were characterized by using contact angle, atomic force microscopy and ellipsometry. After characterization studies, kinetic studies were carried out in the concentration range of 5.0–400.0 μM. The limit of detection and quantification were obtained as 0.0085 and 0.0285 μM, respectively. The response time for the test including equilibration, adsorption and desorption was approximately 9 min. The selectivity studies of the <span style="font-variant: small-caps;">l</span>-phenylalanine imprinted SPR sensor was performed in the presence of <span style="font-variant: small-caps;">d</span>-phenylalanine and <span style="font-variant: small-caps;">l</span>-tryptophan. Validation studies were carried out via enzyme-linked immunosorbent analysis technique in order to demonstrate the applicability and superiority of the <span style="font-variant: small-caps;">l</span>-phenylalanine imprinted SPR sensor.https://www.mdpi.com/2079-6374/11/1/21<span style="font-variant: small-caps">l</span>-phenylalainesurface plasmon resonancesensormolecular imprinting technologydetection |
| spellingShingle | Duygu Çimen Nilay Bereli Adil Denizli Surface Plasmon Resonance Based on Molecularly Imprinted Polymeric Film for <span style="font-variant: small-caps">l</span>-Phenylalanine Detection Biosensors <span style="font-variant: small-caps">l</span>-phenylalaine surface plasmon resonance sensor molecular imprinting technology detection |
| title | Surface Plasmon Resonance Based on Molecularly Imprinted Polymeric Film for <span style="font-variant: small-caps">l</span>-Phenylalanine Detection |
| title_full | Surface Plasmon Resonance Based on Molecularly Imprinted Polymeric Film for <span style="font-variant: small-caps">l</span>-Phenylalanine Detection |
| title_fullStr | Surface Plasmon Resonance Based on Molecularly Imprinted Polymeric Film for <span style="font-variant: small-caps">l</span>-Phenylalanine Detection |
| title_full_unstemmed | Surface Plasmon Resonance Based on Molecularly Imprinted Polymeric Film for <span style="font-variant: small-caps">l</span>-Phenylalanine Detection |
| title_short | Surface Plasmon Resonance Based on Molecularly Imprinted Polymeric Film for <span style="font-variant: small-caps">l</span>-Phenylalanine Detection |
| title_sort | surface plasmon resonance based on molecularly imprinted polymeric film for span style font variant small caps l span phenylalanine detection |
| topic | <span style="font-variant: small-caps">l</span>-phenylalaine surface plasmon resonance sensor molecular imprinting technology detection |
| url | https://www.mdpi.com/2079-6374/11/1/21 |
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