Identification, analysis, and linkage mapping of expressed sequence tags from the Australian sheep blowfly
<p>Abstract</p> <p>Background</p> <p>The Australian sheep blowfly <it>Lucilia cuprina </it>(Wiedemann) (Diptera: Calliphoridae) is a destructive pest of the sheep, a model organism for insecticide resistance research, and a valuable tool for medical and fore...
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| Format: | Article |
| Language: | English |
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BMC
2011-08-01
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| Series: | BMC Genomics |
| Online Access: | http://www.biomedcentral.com/1471-2164/12/406 |
| _version_ | 1818834477850296320 |
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| author | Good Robert T McGrath Annette Chen Zhenzhong Lee Siu F Batterham Philip |
| author_facet | Good Robert T McGrath Annette Chen Zhenzhong Lee Siu F Batterham Philip |
| author_sort | Good Robert T |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The Australian sheep blowfly <it>Lucilia cuprina </it>(Wiedemann) (Diptera: Calliphoridae) is a destructive pest of the sheep, a model organism for insecticide resistance research, and a valuable tool for medical and forensic professionals. However, genomic information on <it>L. cuprina </it>is still sparse.</p> <p>Results</p> <p>We report here the construction of an embryonic and 2 larval cDNA libraries for <it>L. cuprina</it>. A total of 29,816 expressed sequence tags (ESTs) were obtained and assembled into 7,464 unique clusters. The sequence collection captures a great diversity of genes, including those related to insecticide resistance (e.g., 12 cytochrome P450s, 2 glutathione S transferases, and 6 esterases). Compared to <it>Drosophila melanogaster</it>, codon preference is different in 13 of the 18 amino acids encoded by redundant codons, reflecting the lower overall GC content in <it>L. cuprina</it>. In addition, we demonstrated that the ESTs could be converted into informative gene markers by capitalizing on the known gene structures in the model organism <it>D. melanogaster</it>. We successfully assigned 41 genes to their respective chromosomes in <it>L. cuprina</it>. The relative locations of these loci revealed high but incomplete chromosomal synteny between <it>L. cuprina </it>and <it>D. melanogaster</it>.</p> <p>Conclusions</p> <p>Our results represent the first major transcriptomic undertaking in <it>L. cuprina</it>. These new genetic resources could be useful for the blowfly and insect research community.</p> |
| first_indexed | 2024-12-19T02:35:27Z |
| format | Article |
| id | doaj.art-6927e3b75b124ef0aa016e199bd72f8a |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-19T02:35:27Z |
| publishDate | 2011-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-6927e3b75b124ef0aa016e199bd72f8a2022-12-21T20:39:25ZengBMCBMC Genomics1471-21642011-08-0112140610.1186/1471-2164-12-406Identification, analysis, and linkage mapping of expressed sequence tags from the Australian sheep blowflyGood Robert TMcGrath AnnetteChen ZhenzhongLee Siu FBatterham Philip<p>Abstract</p> <p>Background</p> <p>The Australian sheep blowfly <it>Lucilia cuprina </it>(Wiedemann) (Diptera: Calliphoridae) is a destructive pest of the sheep, a model organism for insecticide resistance research, and a valuable tool for medical and forensic professionals. However, genomic information on <it>L. cuprina </it>is still sparse.</p> <p>Results</p> <p>We report here the construction of an embryonic and 2 larval cDNA libraries for <it>L. cuprina</it>. A total of 29,816 expressed sequence tags (ESTs) were obtained and assembled into 7,464 unique clusters. The sequence collection captures a great diversity of genes, including those related to insecticide resistance (e.g., 12 cytochrome P450s, 2 glutathione S transferases, and 6 esterases). Compared to <it>Drosophila melanogaster</it>, codon preference is different in 13 of the 18 amino acids encoded by redundant codons, reflecting the lower overall GC content in <it>L. cuprina</it>. In addition, we demonstrated that the ESTs could be converted into informative gene markers by capitalizing on the known gene structures in the model organism <it>D. melanogaster</it>. We successfully assigned 41 genes to their respective chromosomes in <it>L. cuprina</it>. The relative locations of these loci revealed high but incomplete chromosomal synteny between <it>L. cuprina </it>and <it>D. melanogaster</it>.</p> <p>Conclusions</p> <p>Our results represent the first major transcriptomic undertaking in <it>L. cuprina</it>. These new genetic resources could be useful for the blowfly and insect research community.</p>http://www.biomedcentral.com/1471-2164/12/406 |
| spellingShingle | Good Robert T McGrath Annette Chen Zhenzhong Lee Siu F Batterham Philip Identification, analysis, and linkage mapping of expressed sequence tags from the Australian sheep blowfly BMC Genomics |
| title | Identification, analysis, and linkage mapping of expressed sequence tags from the Australian sheep blowfly |
| title_full | Identification, analysis, and linkage mapping of expressed sequence tags from the Australian sheep blowfly |
| title_fullStr | Identification, analysis, and linkage mapping of expressed sequence tags from the Australian sheep blowfly |
| title_full_unstemmed | Identification, analysis, and linkage mapping of expressed sequence tags from the Australian sheep blowfly |
| title_short | Identification, analysis, and linkage mapping of expressed sequence tags from the Australian sheep blowfly |
| title_sort | identification analysis and linkage mapping of expressed sequence tags from the australian sheep blowfly |
| url | http://www.biomedcentral.com/1471-2164/12/406 |
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