Untargeted Metabolomics of <i>Alternaria solani</i>-Challenged Wild Tomato Species <i>Solanum cheesmaniae</i> Revealed Key Metabolite Biomarkers and Insight into Altered Metabolic Pathways
Untargeted metabolomics of moderately resistant wild tomato species <i>Solanum cheesmaniae</i> revealed an altered metabolite profile in plant leaves in response to <i>Alternaria solani</i> pathogen. Leaf metabolites were significantly differentiated in non-stressed versus st...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-04-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/13/5/585 |
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| author | Dhananjaya Pratap Singh Mansi Singh Bisen Ratna Prabha Sudarshan Maurya Suresh Reddy Yerasu Renu Shukla Jagesh Kumar Tiwari Krishna Kumar Chaturvedi Md. Samir Farooqi Sudhir Srivastava Anil Rai Birinchi Kumar Sarma Nagendra Rai Prabhakar Mohan Singh Tusar Kanti Behera Mohamed A. Farag |
| author_facet | Dhananjaya Pratap Singh Mansi Singh Bisen Ratna Prabha Sudarshan Maurya Suresh Reddy Yerasu Renu Shukla Jagesh Kumar Tiwari Krishna Kumar Chaturvedi Md. Samir Farooqi Sudhir Srivastava Anil Rai Birinchi Kumar Sarma Nagendra Rai Prabhakar Mohan Singh Tusar Kanti Behera Mohamed A. Farag |
| author_sort | Dhananjaya Pratap Singh |
| collection | DOAJ |
| description | Untargeted metabolomics of moderately resistant wild tomato species <i>Solanum cheesmaniae</i> revealed an altered metabolite profile in plant leaves in response to <i>Alternaria solani</i> pathogen. Leaf metabolites were significantly differentiated in non-stressed versus stressed plants. The samples were discriminated not only by the presence/absence of specific metabolites as distinguished markers of infection, but also on the basis of their relative abundance as important concluding factors. Annotation of metabolite features using the <i>Arabidopsis thaliana</i> (KEGG) database revealed 3371 compounds with KEGG identifiers belonging to biosynthetic pathways including secondary metabolites, cofactors, steroids, brassinosteroids, terpernoids, and fatty acids. Annotation using the <i>Solanum lycopersicum</i> database in PLANTCYC PMN revealed significantly upregulated (541) and downregulated (485) features distributed in metabolite classes that appeared to play a crucial role in defense, infection prevention, signaling, plant growth, and plant homeostasis to survive under stress conditions. The orthogonal partial least squares discriminant analysis (OPLS-DA), comprising a significant fold change (≥2.0) with VIP score (≥1.0), showed 34 upregulated biomarker metabolites including 5-phosphoribosylamine, kaur-16-en-18-oic acid, pantothenate, and <i>O</i>-acetyl-L-homoserine, along with 41 downregulated biomarkers. Downregulated metabolite biomarkers were mapped with pathways specifically known for plant defense, suggesting their prominent role in pathogen resistance. These results hold promise for identifying key biomarker metabolites that contribute to disease resistive metabolic traits/biosynthetic routes. This approach can assist in mQTL development for the stress breeding program in tomato against pathogen interactions. |
| first_indexed | 2024-03-11T03:30:21Z |
| format | Article |
| id | doaj.art-692916e1ccf84dcba33b32d385ceabde |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-11T03:30:21Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-692916e1ccf84dcba33b32d385ceabde2023-11-18T02:24:57ZengMDPI AGMetabolites2218-19892023-04-0113558510.3390/metabo13050585Untargeted Metabolomics of <i>Alternaria solani</i>-Challenged Wild Tomato Species <i>Solanum cheesmaniae</i> Revealed Key Metabolite Biomarkers and Insight into Altered Metabolic PathwaysDhananjaya Pratap Singh0Mansi Singh Bisen1Ratna Prabha2Sudarshan Maurya3Suresh Reddy Yerasu4Renu Shukla5Jagesh Kumar Tiwari6Krishna Kumar Chaturvedi7Md. Samir Farooqi8Sudhir Srivastava9Anil Rai10Birinchi Kumar Sarma11Nagendra Rai12Prabhakar Mohan Singh13Tusar Kanti Behera14Mohamed A. Farag15ICAR-Indian Institute of Vegetable Research, Varanasi 221305, IndiaICAR-Indian Institute of Vegetable Research, Varanasi 221305, IndiaICAR-Indian Agricultural Statistics Research Institute, Library Avenue, New Delhi 110012, IndiaICAR-Indian Institute of Vegetable Research, Varanasi 221305, IndiaICAR-Indian Institute of Vegetable Research, Varanasi 221305, IndiaIndian Council of Agricultural Research, New Delhi 110012, IndiaICAR-Indian Institute of Vegetable Research, Varanasi 221305, IndiaICAR-Indian Agricultural Statistics Research Institute, Library Avenue, New Delhi 110012, IndiaICAR-Indian Agricultural Statistics Research Institute, Library Avenue, New Delhi 110012, IndiaICAR-Indian Agricultural Statistics Research Institute, Library Avenue, New Delhi 110012, IndiaICAR-Indian Agricultural Statistics Research Institute, Library Avenue, New Delhi 110012, IndiaDepartment of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi 221005, IndiaICAR-Indian Institute of Vegetable Research, Varanasi 221305, IndiaICAR-Indian Institute of Vegetable Research, Varanasi 221305, IndiaICAR-Indian Institute of Vegetable Research, Varanasi 221305, IndiaPharmacognosy Department, College of Pharmacy, Cairo University, Cairo 11562, EgyptUntargeted metabolomics of moderately resistant wild tomato species <i>Solanum cheesmaniae</i> revealed an altered metabolite profile in plant leaves in response to <i>Alternaria solani</i> pathogen. Leaf metabolites were significantly differentiated in non-stressed versus stressed plants. The samples were discriminated not only by the presence/absence of specific metabolites as distinguished markers of infection, but also on the basis of their relative abundance as important concluding factors. Annotation of metabolite features using the <i>Arabidopsis thaliana</i> (KEGG) database revealed 3371 compounds with KEGG identifiers belonging to biosynthetic pathways including secondary metabolites, cofactors, steroids, brassinosteroids, terpernoids, and fatty acids. Annotation using the <i>Solanum lycopersicum</i> database in PLANTCYC PMN revealed significantly upregulated (541) and downregulated (485) features distributed in metabolite classes that appeared to play a crucial role in defense, infection prevention, signaling, plant growth, and plant homeostasis to survive under stress conditions. The orthogonal partial least squares discriminant analysis (OPLS-DA), comprising a significant fold change (≥2.0) with VIP score (≥1.0), showed 34 upregulated biomarker metabolites including 5-phosphoribosylamine, kaur-16-en-18-oic acid, pantothenate, and <i>O</i>-acetyl-L-homoserine, along with 41 downregulated biomarkers. Downregulated metabolite biomarkers were mapped with pathways specifically known for plant defense, suggesting their prominent role in pathogen resistance. These results hold promise for identifying key biomarker metabolites that contribute to disease resistive metabolic traits/biosynthetic routes. This approach can assist in mQTL development for the stress breeding program in tomato against pathogen interactions.https://www.mdpi.com/2218-1989/13/5/585metabolite profilingsecondary metabolitesmetabolomicsbiotic stress<i>Solanum cheesmaniae</i><i>Alternaria solani</i> |
| spellingShingle | Dhananjaya Pratap Singh Mansi Singh Bisen Ratna Prabha Sudarshan Maurya Suresh Reddy Yerasu Renu Shukla Jagesh Kumar Tiwari Krishna Kumar Chaturvedi Md. Samir Farooqi Sudhir Srivastava Anil Rai Birinchi Kumar Sarma Nagendra Rai Prabhakar Mohan Singh Tusar Kanti Behera Mohamed A. Farag Untargeted Metabolomics of <i>Alternaria solani</i>-Challenged Wild Tomato Species <i>Solanum cheesmaniae</i> Revealed Key Metabolite Biomarkers and Insight into Altered Metabolic Pathways Metabolites metabolite profiling secondary metabolites metabolomics biotic stress <i>Solanum cheesmaniae</i> <i>Alternaria solani</i> |
| title | Untargeted Metabolomics of <i>Alternaria solani</i>-Challenged Wild Tomato Species <i>Solanum cheesmaniae</i> Revealed Key Metabolite Biomarkers and Insight into Altered Metabolic Pathways |
| title_full | Untargeted Metabolomics of <i>Alternaria solani</i>-Challenged Wild Tomato Species <i>Solanum cheesmaniae</i> Revealed Key Metabolite Biomarkers and Insight into Altered Metabolic Pathways |
| title_fullStr | Untargeted Metabolomics of <i>Alternaria solani</i>-Challenged Wild Tomato Species <i>Solanum cheesmaniae</i> Revealed Key Metabolite Biomarkers and Insight into Altered Metabolic Pathways |
| title_full_unstemmed | Untargeted Metabolomics of <i>Alternaria solani</i>-Challenged Wild Tomato Species <i>Solanum cheesmaniae</i> Revealed Key Metabolite Biomarkers and Insight into Altered Metabolic Pathways |
| title_short | Untargeted Metabolomics of <i>Alternaria solani</i>-Challenged Wild Tomato Species <i>Solanum cheesmaniae</i> Revealed Key Metabolite Biomarkers and Insight into Altered Metabolic Pathways |
| title_sort | untargeted metabolomics of i alternaria solani i challenged wild tomato species i solanum cheesmaniae i revealed key metabolite biomarkers and insight into altered metabolic pathways |
| topic | metabolite profiling secondary metabolites metabolomics biotic stress <i>Solanum cheesmaniae</i> <i>Alternaria solani</i> |
| url | https://www.mdpi.com/2218-1989/13/5/585 |
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