Bioinformatics and Experimental Evaluation of UBE2W and SSX2IP Expression Levels in HepG2 Cancer Cells Treated with Melatonin
Background and purpose: The aim of this study was to bioinformatically and experimentally evaluate the effect of melatonin on the expression levels of UBE2W and SSX2IP genes in melatonin treated Human Hepatocellular carcinoma G2 (HepG2) cancer cell line. UBE2W encodes a protein that promotes ubiquit...
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| Format: | Article |
| Language: | English |
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Mazandaran University of Medical Sciences
2021-01-01
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| Series: | Journal of Mazandaran University of Medical Sciences |
| Subjects: | |
| Online Access: | http://jmums.mazums.ac.ir/article-1-15237-en.html |
| _version_ | 1797958879382863872 |
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| author | Ali Rajabi Ali Emami Behnaz Barzegarzadeh Namarvar Reza Safaralizadeh |
| author_facet | Ali Rajabi Ali Emami Behnaz Barzegarzadeh Namarvar Reza Safaralizadeh |
| author_sort | Ali Rajabi |
| collection | DOAJ |
| description | Background and purpose: The aim of this study was to bioinformatically and experimentally evaluate the effect of melatonin on the expression levels of UBE2W and SSX2IP genes in melatonin treated Human Hepatocellular carcinoma G2 (HepG2) cancer cell line. UBE2W encodes a protein that promotes ubiquitination of Fanconi anemia complementation group proteins and may be important in the repair of DNA damage. SSX2IP belongs to an adhesion system, involved in cell movement and acts as a centrosome maturation factor.
Materials and methods: At first, as a bioinformatics tool, MATLAB 2018a software was employed to evaluate the UBE2W and SSX2IP genes expression levels using microarray data. Then, after designing and preparing the primers, MTT and Real-time PCR were carried out in three replicates.
Results: MTT assay showed that the viability of cancer cell significantly decreased in melatonin treated group (P≤0.01). The IC50 value was estimated to be 2080 µM for 48 hours, but no significant changes were seen in the survival of human normal cells (HUVEC-C) (P≥0.05). Real-time PCR proved that the expression levels of both genes increased (P≥0.05 in 24h and P≤ 0.05 in 48h) in melatonin treated cells compared to un-treated group, which was in accordance with bioinformatics analysis.
Conclusion: Our study showed that UBE2W and SSX2IP genes could be used as therapeutic target genes for Hepatocellular carcinoma. However, complementary studies are needed to prove current findings. |
| first_indexed | 2024-04-11T00:25:05Z |
| format | Article |
| id | doaj.art-692b54f4849d40d0887352ee255d7694 |
| institution | Directory Open Access Journal |
| issn | 1735-9260 1735-9279 |
| language | English |
| last_indexed | 2024-04-11T00:25:05Z |
| publishDate | 2021-01-01 |
| publisher | Mazandaran University of Medical Sciences |
| record_format | Article |
| series | Journal of Mazandaran University of Medical Sciences |
| spelling | doaj.art-692b54f4849d40d0887352ee255d76942023-01-08T08:43:29ZengMazandaran University of Medical SciencesJournal of Mazandaran University of Medical Sciences1735-92601735-92792021-01-01301921223Bioinformatics and Experimental Evaluation of UBE2W and SSX2IP Expression Levels in HepG2 Cancer Cells Treated with MelatoninAli Rajabi0Ali Emami1Behnaz Barzegarzadeh Namarvar2Reza Safaralizadeh3 PhD Student in Genetics, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran MSc in Genetics, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran MSc in Animal Physiology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran Associate Professor, Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran Background and purpose: The aim of this study was to bioinformatically and experimentally evaluate the effect of melatonin on the expression levels of UBE2W and SSX2IP genes in melatonin treated Human Hepatocellular carcinoma G2 (HepG2) cancer cell line. UBE2W encodes a protein that promotes ubiquitination of Fanconi anemia complementation group proteins and may be important in the repair of DNA damage. SSX2IP belongs to an adhesion system, involved in cell movement and acts as a centrosome maturation factor. Materials and methods: At first, as a bioinformatics tool, MATLAB 2018a software was employed to evaluate the UBE2W and SSX2IP genes expression levels using microarray data. Then, after designing and preparing the primers, MTT and Real-time PCR were carried out in three replicates. Results: MTT assay showed that the viability of cancer cell significantly decreased in melatonin treated group (P≤0.01). The IC50 value was estimated to be 2080 µM for 48 hours, but no significant changes were seen in the survival of human normal cells (HUVEC-C) (P≥0.05). Real-time PCR proved that the expression levels of both genes increased (P≥0.05 in 24h and P≤ 0.05 in 48h) in melatonin treated cells compared to un-treated group, which was in accordance with bioinformatics analysis. Conclusion: Our study showed that UBE2W and SSX2IP genes could be used as therapeutic target genes for Hepatocellular carcinoma. However, complementary studies are needed to prove current findings.http://jmums.mazums.ac.ir/article-1-15237-en.htmlmelatoninhepatocellular carcinomabioinformaticsmicroarray data |
| spellingShingle | Ali Rajabi Ali Emami Behnaz Barzegarzadeh Namarvar Reza Safaralizadeh Bioinformatics and Experimental Evaluation of UBE2W and SSX2IP Expression Levels in HepG2 Cancer Cells Treated with Melatonin Journal of Mazandaran University of Medical Sciences melatonin hepatocellular carcinoma bioinformatics microarray data |
| title | Bioinformatics and Experimental Evaluation of UBE2W and SSX2IP Expression Levels in HepG2 Cancer
Cells Treated with Melatonin |
| title_full | Bioinformatics and Experimental Evaluation of UBE2W and SSX2IP Expression Levels in HepG2 Cancer
Cells Treated with Melatonin |
| title_fullStr | Bioinformatics and Experimental Evaluation of UBE2W and SSX2IP Expression Levels in HepG2 Cancer
Cells Treated with Melatonin |
| title_full_unstemmed | Bioinformatics and Experimental Evaluation of UBE2W and SSX2IP Expression Levels in HepG2 Cancer
Cells Treated with Melatonin |
| title_short | Bioinformatics and Experimental Evaluation of UBE2W and SSX2IP Expression Levels in HepG2 Cancer
Cells Treated with Melatonin |
| title_sort | bioinformatics and experimental evaluation of ube2w and ssx2ip expression levels in hepg2 cancer cells treated with melatonin |
| topic | melatonin hepatocellular carcinoma bioinformatics microarray data |
| url | http://jmums.mazums.ac.ir/article-1-15237-en.html |
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