From the RNA-Peptide World: Prebiotic Reaction Conditions Compatible with Lipid Membranes for the Formation of Lipophilic Random Peptides in the Presence of Short Oligonucleotides, and More
Deciphering the origins of life on a molecular level includes unravelling the numerous interactions that could occur between the most important biomolecules being the lipids, peptides and nucleotides. They were likely all present on the early Earth and all necessary for the emergence of cellular lif...
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| Format: | Article |
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MDPI AG
2024-01-01
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| Series: | Life |
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| Online Access: | https://www.mdpi.com/2075-1729/14/1/108 |
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| author | Augustin Lopez Antoine Vauchez Ghinwa Ajram Anastasiia Shvetsova Gabrielle Leveau Michele Fiore Peter Strazewski |
| author_facet | Augustin Lopez Antoine Vauchez Ghinwa Ajram Anastasiia Shvetsova Gabrielle Leveau Michele Fiore Peter Strazewski |
| author_sort | Augustin Lopez |
| collection | DOAJ |
| description | Deciphering the origins of life on a molecular level includes unravelling the numerous interactions that could occur between the most important biomolecules being the lipids, peptides and nucleotides. They were likely all present on the early Earth and all necessary for the emergence of cellular life. In this study, we intended to explore conditions that were at the same time conducive to chemical reactions critical for the origins of life (peptide–oligonucleotide couplings and templated ligation of oligonucleotides) and compatible with the presence of prebiotic lipid vesicles. For that, random peptides were generated from activated amino acids and analysed using NMR and MS, whereas short oligonucleotides were produced through solid-support synthesis, manually deprotected and purified using HPLC. After chemical activation in prebiotic conditions, the resulting mixtures were analysed using LC-MS. Vesicles could be produced through gentle hydration in similar conditions and observed using epifluorescence microscopy. Despite the absence of coupling or ligation, our results help to pave the way for future investigations on the origins of life that may gather all three types of biomolecules rather than studying them separately, as it is still too often the case. |
| first_indexed | 2024-03-08T10:44:22Z |
| format | Article |
| id | doaj.art-692b58fc4be84f02a3d31751161e057c |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-08T10:44:22Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-692b58fc4be84f02a3d31751161e057c2024-01-26T17:21:03ZengMDPI AGLife2075-17292024-01-0114110810.3390/life14010108From the RNA-Peptide World: Prebiotic Reaction Conditions Compatible with Lipid Membranes for the Formation of Lipophilic Random Peptides in the Presence of Short Oligonucleotides, and MoreAugustin Lopez0Antoine Vauchez1Ghinwa Ajram2Anastasiia Shvetsova3Gabrielle Leveau4Michele Fiore5Peter Strazewski6Laboratoire de Chimie Organique 2 (LCO2), Institut de Chimie et Biochimie Moléculaires et Supramoléculaires (ICBMS, UMR CNRS 5246), Bâtiment Edgar Lederer, Université Claude Bernard Lyon 1, Université de Lyon, 1 rue Victor Grignard, 69100 Villeurbanne, FranceCentre Commun de la Spectrométrie de Masse (CCSM), ICBMS, Bâtiment Edgar Lederer, 1 rue Victor Grignard, 69100 Villeurbanne, FranceLaboratoire de Chimie Organique 2 (LCO2), Institut de Chimie et Biochimie Moléculaires et Supramoléculaires (ICBMS, UMR CNRS 5246), Bâtiment Edgar Lederer, Université Claude Bernard Lyon 1, Université de Lyon, 1 rue Victor Grignard, 69100 Villeurbanne, FranceLaboratoire de Chimie Organique 2 (LCO2), Institut de Chimie et Biochimie Moléculaires et Supramoléculaires (ICBMS, UMR CNRS 5246), Bâtiment Edgar Lederer, Université Claude Bernard Lyon 1, Université de Lyon, 1 rue Victor Grignard, 69100 Villeurbanne, FranceLaboratoire de Chimie Organique 2 (LCO2), Institut de Chimie et Biochimie Moléculaires et Supramoléculaires (ICBMS, UMR CNRS 5246), Bâtiment Edgar Lederer, Université Claude Bernard Lyon 1, Université de Lyon, 1 rue Victor Grignard, 69100 Villeurbanne, FranceLaboratoire de Chimie Organique 2 (LCO2), Institut de Chimie et Biochimie Moléculaires et Supramoléculaires (ICBMS, UMR CNRS 5246), Bâtiment Edgar Lederer, Université Claude Bernard Lyon 1, Université de Lyon, 1 rue Victor Grignard, 69100 Villeurbanne, FranceLaboratoire de Chimie Organique 2 (LCO2), Institut de Chimie et Biochimie Moléculaires et Supramoléculaires (ICBMS, UMR CNRS 5246), Bâtiment Edgar Lederer, Université Claude Bernard Lyon 1, Université de Lyon, 1 rue Victor Grignard, 69100 Villeurbanne, FranceDeciphering the origins of life on a molecular level includes unravelling the numerous interactions that could occur between the most important biomolecules being the lipids, peptides and nucleotides. They were likely all present on the early Earth and all necessary for the emergence of cellular life. In this study, we intended to explore conditions that were at the same time conducive to chemical reactions critical for the origins of life (peptide–oligonucleotide couplings and templated ligation of oligonucleotides) and compatible with the presence of prebiotic lipid vesicles. For that, random peptides were generated from activated amino acids and analysed using NMR and MS, whereas short oligonucleotides were produced through solid-support synthesis, manually deprotected and purified using HPLC. After chemical activation in prebiotic conditions, the resulting mixtures were analysed using LC-MS. Vesicles could be produced through gentle hydration in similar conditions and observed using epifluorescence microscopy. Despite the absence of coupling or ligation, our results help to pave the way for future investigations on the origins of life that may gather all three types of biomolecules rather than studying them separately, as it is still too often the case.https://www.mdpi.com/2075-1729/14/1/108chimerasDNAhot springsligationlipidsoligonucleotides |
| spellingShingle | Augustin Lopez Antoine Vauchez Ghinwa Ajram Anastasiia Shvetsova Gabrielle Leveau Michele Fiore Peter Strazewski From the RNA-Peptide World: Prebiotic Reaction Conditions Compatible with Lipid Membranes for the Formation of Lipophilic Random Peptides in the Presence of Short Oligonucleotides, and More Life chimeras DNA hot springs ligation lipids oligonucleotides |
| title | From the RNA-Peptide World: Prebiotic Reaction Conditions Compatible with Lipid Membranes for the Formation of Lipophilic Random Peptides in the Presence of Short Oligonucleotides, and More |
| title_full | From the RNA-Peptide World: Prebiotic Reaction Conditions Compatible with Lipid Membranes for the Formation of Lipophilic Random Peptides in the Presence of Short Oligonucleotides, and More |
| title_fullStr | From the RNA-Peptide World: Prebiotic Reaction Conditions Compatible with Lipid Membranes for the Formation of Lipophilic Random Peptides in the Presence of Short Oligonucleotides, and More |
| title_full_unstemmed | From the RNA-Peptide World: Prebiotic Reaction Conditions Compatible with Lipid Membranes for the Formation of Lipophilic Random Peptides in the Presence of Short Oligonucleotides, and More |
| title_short | From the RNA-Peptide World: Prebiotic Reaction Conditions Compatible with Lipid Membranes for the Formation of Lipophilic Random Peptides in the Presence of Short Oligonucleotides, and More |
| title_sort | from the rna peptide world prebiotic reaction conditions compatible with lipid membranes for the formation of lipophilic random peptides in the presence of short oligonucleotides and more |
| topic | chimeras DNA hot springs ligation lipids oligonucleotides |
| url | https://www.mdpi.com/2075-1729/14/1/108 |
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