Delivery of Nanoparticles across the Intestinal Epithelium via the Transferrin Transport Pathway
The aim of this study was to probe whether the transferrin (Tf) transport pathway can be exploited for intestinal delivery of nanoparticles. Tf was adsorbed on 100 nm model polystyrene nanoparticles (NP), followed by size characterisation of these systems. Cell uptake of Tf and Tf-adsorbed NP was in...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-06-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/11/7/298 |
| _version_ | 1828283919852634112 |
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| author | Jing M. Yong Julia Mantaj Yiyi Cheng Driton Vllasaliu |
| author_facet | Jing M. Yong Julia Mantaj Yiyi Cheng Driton Vllasaliu |
| author_sort | Jing M. Yong |
| collection | DOAJ |
| description | The aim of this study was to probe whether the transferrin (Tf) transport pathway can be exploited for intestinal delivery of nanoparticles. Tf was adsorbed on 100 nm model polystyrene nanoparticles (NP), followed by size characterisation of these systems. Cell uptake of Tf and Tf-adsorbed NP was investigated in intestinal epithelial Caco-2 cells cultured on multi-well plates and as differentiated polarised monolayers. Tf-NP demonstrated a remarkably higher cell uptake compared to unmodified NP in both non-polarised (5-fold) and polarised cell monolayers (16-fold difference). Application of soluble Tf significantly attenuated the uptake of Tf-NP. Notably, Tf-NP displayed remarkably higher rate (23-fold) of epithelial transport across Caco-2 monolayers compared to unmodified NP. This study therefore strongly suggests that the Tf transport pathway should be considered as a candidate biological transport route for orally-administered nanomedicines and drugs with poor oral bioavailability. |
| first_indexed | 2024-04-13T08:53:10Z |
| format | Article |
| id | doaj.art-692c212831d74f359cba90d6647e4798 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-04-13T08:53:10Z |
| publishDate | 2019-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-692c212831d74f359cba90d6647e47982022-12-22T02:53:25ZengMDPI AGPharmaceutics1999-49232019-06-0111729810.3390/pharmaceutics11070298pharmaceutics11070298Delivery of Nanoparticles across the Intestinal Epithelium via the Transferrin Transport PathwayJing M. Yong0Julia Mantaj1Yiyi Cheng2Driton Vllasaliu3School of Cancer and Pharmaceutical Sciences, Faculty of Life Sciences & Medicine, King’s College London, London SE1 9NH, UKSchool of Cancer and Pharmaceutical Sciences, Faculty of Life Sciences & Medicine, King’s College London, London SE1 9NH, UKSchool of Cancer and Pharmaceutical Sciences, Faculty of Life Sciences & Medicine, King’s College London, London SE1 9NH, UKSchool of Cancer and Pharmaceutical Sciences, Faculty of Life Sciences & Medicine, King’s College London, London SE1 9NH, UKThe aim of this study was to probe whether the transferrin (Tf) transport pathway can be exploited for intestinal delivery of nanoparticles. Tf was adsorbed on 100 nm model polystyrene nanoparticles (NP), followed by size characterisation of these systems. Cell uptake of Tf and Tf-adsorbed NP was investigated in intestinal epithelial Caco-2 cells cultured on multi-well plates and as differentiated polarised monolayers. Tf-NP demonstrated a remarkably higher cell uptake compared to unmodified NP in both non-polarised (5-fold) and polarised cell monolayers (16-fold difference). Application of soluble Tf significantly attenuated the uptake of Tf-NP. Notably, Tf-NP displayed remarkably higher rate (23-fold) of epithelial transport across Caco-2 monolayers compared to unmodified NP. This study therefore strongly suggests that the Tf transport pathway should be considered as a candidate biological transport route for orally-administered nanomedicines and drugs with poor oral bioavailability.https://www.mdpi.com/1999-4923/11/7/298Caco-2intestinal absorptionnanomedicinenanoparticleoral deliverytransferrin |
| spellingShingle | Jing M. Yong Julia Mantaj Yiyi Cheng Driton Vllasaliu Delivery of Nanoparticles across the Intestinal Epithelium via the Transferrin Transport Pathway Pharmaceutics Caco-2 intestinal absorption nanomedicine nanoparticle oral delivery transferrin |
| title | Delivery of Nanoparticles across the Intestinal Epithelium via the Transferrin Transport Pathway |
| title_full | Delivery of Nanoparticles across the Intestinal Epithelium via the Transferrin Transport Pathway |
| title_fullStr | Delivery of Nanoparticles across the Intestinal Epithelium via the Transferrin Transport Pathway |
| title_full_unstemmed | Delivery of Nanoparticles across the Intestinal Epithelium via the Transferrin Transport Pathway |
| title_short | Delivery of Nanoparticles across the Intestinal Epithelium via the Transferrin Transport Pathway |
| title_sort | delivery of nanoparticles across the intestinal epithelium via the transferrin transport pathway |
| topic | Caco-2 intestinal absorption nanomedicine nanoparticle oral delivery transferrin |
| url | https://www.mdpi.com/1999-4923/11/7/298 |
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