Phenotyping Fusarium head blight through seed morphology characteristics using RGB imaging
Fusarium head blight (FHB) is an economically important disease affecting wheat and thus poses a major threat to wheat production. Several studies have evaluated the effectiveness of image analysis methods to predict FHB using disease-infected grains; however, few have looked at the final applicatio...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.1010249/full |
| _version_ | 1798030122778886144 |
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| author | Fernanda Leiva Mustafa Zakieh Marwan Alamrani Rishap Dhakal Tina Henriksson Pawan Kumar Singh Aakash Chawade |
| author_facet | Fernanda Leiva Mustafa Zakieh Marwan Alamrani Rishap Dhakal Tina Henriksson Pawan Kumar Singh Aakash Chawade |
| author_sort | Fernanda Leiva |
| collection | DOAJ |
| description | Fusarium head blight (FHB) is an economically important disease affecting wheat and thus poses a major threat to wheat production. Several studies have evaluated the effectiveness of image analysis methods to predict FHB using disease-infected grains; however, few have looked at the final application, considering the relationship between cost and benefit, resolution, and accuracy. The conventional screening of FHB resistance of large-scale samples is still dependent on low-throughput visual inspections. This study aims to compare the performance of two cost–benefit seed image analysis methods, the free software “SmartGrain” and the fully automated commercially available instrument “Cgrain Value™” by assessing 16 seed morphological traits of winter wheat to predict FHB. The analysis was carried out on a seed set of FHB which was visually assessed as to the severity. The dataset is composed of 432 winter wheat genotypes that were greenhouse-inoculated. The predictions from each method, in addition to the predictions combined from the results of both methods, were compared with the disease visual scores. The results showed that Cgrain Value™ had a higher prediction accuracy of R2 = 0.52 compared with SmartGrain for which R2 = 0.30 for all morphological traits. However, the results combined from both methods showed the greatest prediction performance of R2 = 0.58. Additionally, a subpart of the morphological traits, namely, width, length, thickness, and color features, showed a higher correlation with the visual scores compared with the other traits. Overall, both methods were related to the visual scores. This study shows that these affordable imaging methods could be effective to predict FHB in seeds and enable us to distinguish minor differences in seed morphology, which could lead to a precise performance selection of disease-free seeds/grains. |
| first_indexed | 2024-04-11T19:35:07Z |
| format | Article |
| id | doaj.art-692c4eed62da40ce9d248770859da942 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-11T19:35:07Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-692c4eed62da40ce9d248770859da9422022-12-22T04:06:52ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-10-011310.3389/fpls.2022.10102491010249Phenotyping Fusarium head blight through seed morphology characteristics using RGB imagingFernanda Leiva0Mustafa Zakieh1Marwan Alamrani2Rishap Dhakal3Tina Henriksson4Pawan Kumar Singh5Aakash Chawade6Department of Plant Breeding, Swedish University of Agricultural Sciences, Lomma, SwedenDepartment of Plant Breeding, Swedish University of Agricultural Sciences, Lomma, SwedenDepartment of Plant Breeding, Swedish University of Agricultural Sciences, Lomma, SwedenDepartment of Plant Breeding, Swedish University of Agricultural Sciences, Lomma, SwedenLantmännen Lantbruk, Svalöv, SwedenInternational Maize and Wheat Improvement Center (CIMMYT), Texcoco, MexicoDepartment of Plant Breeding, Swedish University of Agricultural Sciences, Lomma, SwedenFusarium head blight (FHB) is an economically important disease affecting wheat and thus poses a major threat to wheat production. Several studies have evaluated the effectiveness of image analysis methods to predict FHB using disease-infected grains; however, few have looked at the final application, considering the relationship between cost and benefit, resolution, and accuracy. The conventional screening of FHB resistance of large-scale samples is still dependent on low-throughput visual inspections. This study aims to compare the performance of two cost–benefit seed image analysis methods, the free software “SmartGrain” and the fully automated commercially available instrument “Cgrain Value™” by assessing 16 seed morphological traits of winter wheat to predict FHB. The analysis was carried out on a seed set of FHB which was visually assessed as to the severity. The dataset is composed of 432 winter wheat genotypes that were greenhouse-inoculated. The predictions from each method, in addition to the predictions combined from the results of both methods, were compared with the disease visual scores. The results showed that Cgrain Value™ had a higher prediction accuracy of R2 = 0.52 compared with SmartGrain for which R2 = 0.30 for all morphological traits. However, the results combined from both methods showed the greatest prediction performance of R2 = 0.58. Additionally, a subpart of the morphological traits, namely, width, length, thickness, and color features, showed a higher correlation with the visual scores compared with the other traits. Overall, both methods were related to the visual scores. This study shows that these affordable imaging methods could be effective to predict FHB in seeds and enable us to distinguish minor differences in seed morphology, which could lead to a precise performance selection of disease-free seeds/grains.https://www.frontiersin.org/articles/10.3389/fpls.2022.1010249/fullFusarium head blightseed phenotypingseed morphological characterswheatvisual scoresSmartGrain |
| spellingShingle | Fernanda Leiva Mustafa Zakieh Marwan Alamrani Rishap Dhakal Tina Henriksson Pawan Kumar Singh Aakash Chawade Phenotyping Fusarium head blight through seed morphology characteristics using RGB imaging Frontiers in Plant Science Fusarium head blight seed phenotyping seed morphological characters wheat visual scores SmartGrain |
| title | Phenotyping Fusarium head blight through seed morphology characteristics using RGB imaging |
| title_full | Phenotyping Fusarium head blight through seed morphology characteristics using RGB imaging |
| title_fullStr | Phenotyping Fusarium head blight through seed morphology characteristics using RGB imaging |
| title_full_unstemmed | Phenotyping Fusarium head blight through seed morphology characteristics using RGB imaging |
| title_short | Phenotyping Fusarium head blight through seed morphology characteristics using RGB imaging |
| title_sort | phenotyping fusarium head blight through seed morphology characteristics using rgb imaging |
| topic | Fusarium head blight seed phenotyping seed morphological characters wheat visual scores SmartGrain |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.1010249/full |
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