Colonization with selected antibiotic resistant bacteria among a cohort of Sri Lankan university students
Abstract Background Antibiotic Resistance is an imminent global public health threat. Antibiotic resistance emerged in healthcare settings and has now moved on to the community settings. This study was conducted to identify the rates of asymptomatic colonization with selected antibiotic resistant or...
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| Format: | Article |
| Language: | English |
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BMC
2021-06-01
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| Series: | BMC Infectious Diseases |
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| Online Access: | https://doi.org/10.1186/s12879-021-06289-z |
| _version_ | 1819105986683600896 |
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| author | Thilini Munasinghe Gihani Vidanapathirana Shahlina Kuthubdeen Asela Ekanayake Sacheera Angulmaduwa Kunchana De Silva Susan Subhasinghe Ruwani Kalupahana Veranja Liyanapathirana Margaret Ip |
| author_facet | Thilini Munasinghe Gihani Vidanapathirana Shahlina Kuthubdeen Asela Ekanayake Sacheera Angulmaduwa Kunchana De Silva Susan Subhasinghe Ruwani Kalupahana Veranja Liyanapathirana Margaret Ip |
| author_sort | Thilini Munasinghe |
| collection | DOAJ |
| description | Abstract Background Antibiotic Resistance is an imminent global public health threat. Antibiotic resistance emerged in healthcare settings and has now moved on to the community settings. This study was conducted to identify the rates of asymptomatic colonization with selected antibiotic resistant organisms, (Methicillin Resistant Staphylococcus aureus (MRSA), Extended Spectrum Beta Lactamase (ESBL) producing Escherichia coli and Klebsiella spp and carbapenem resistant E.coli and Klebsiella spp) - among a group of university students in Sri Lanka. Identification of genetic determinants of MRSA and ESBL was an additional objective of the study. Methods A self - collected nasal swab and a peri-rectal swab collected after passing stools were obtained. Routine microbiological methods were used for the isolation S.aureus from the nasal swab and E.coli and Klebsiella species from the peri-rectal swab. Antibiotic sensitivity testing was performed as recommended by clinical and laboratory standard institute (CLSI). Three (3) genes that are responsible for ESBL production; bla CTX-M, bla SHV, and bla TEM were tested using previously described primers and PCR procedures. Identification of MecA and PVL genes attributed to MRSA was also done with PCR. Results A total of 322 participants between 21 and 28 years were recruited representing 5 different faculties of study. Seventy one (22.0%) were colonized with S.aureus and 14 among them with MRSA, making the MRSA colonization rate of 4.3%. Forty five (15%) of the participants were colonized with an ESBL producing E.coli or Klebsiella spp. No one was colonized with carbapenem resistant E.coli or Klebsiella species. Of the 45 ESBL producers the commonest genetic determinant identified was bla CTX-M (n = 36), while 16 isolates had bla TEM and 7 had bla SHV. Similarly, of the 14 isolates identified as MRSA, 3 (21.4%) were found to be PVL positive while 11 (78.6%) were MecA positive. Conclusions A high rate of colonization with ESBL producing E.coli and Klebsiella species was noted in our study group. |
| first_indexed | 2024-12-22T02:30:58Z |
| format | Article |
| id | doaj.art-69306db66c1c478fbf94d218f339bbca |
| institution | Directory Open Access Journal |
| issn | 1471-2334 |
| language | English |
| last_indexed | 2024-12-22T02:30:58Z |
| publishDate | 2021-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Infectious Diseases |
| spelling | doaj.art-69306db66c1c478fbf94d218f339bbca2022-12-21T18:41:53ZengBMCBMC Infectious Diseases1471-23342021-06-012111810.1186/s12879-021-06289-zColonization with selected antibiotic resistant bacteria among a cohort of Sri Lankan university studentsThilini Munasinghe0Gihani Vidanapathirana1Shahlina Kuthubdeen2Asela Ekanayake3Sacheera Angulmaduwa4Kunchana De Silva5Susan Subhasinghe6Ruwani Kalupahana7Veranja Liyanapathirana8Margaret Ip9Postgraduate Institute of Science, University of PeradeniyaFaculty of Medicine, University of PeradeniyaPostgraduate Institute of Science, University of PeradeniyaDepartment of Microbiology, Faculty of Medicine, University of PeradeniyaDepartment of Microbiology, Faculty of Medicine, University of PeradeniyaDepartment of Microbiology, Faculty of Medicine, University of PeradeniyaFaculty of Medicine, University of PeradeniyaDepartment of Veterinary Public Health and Pharmacology, Faculty of Veterinary Medicine and Animal Science, University of PeradeniyaDepartment of Microbiology, Faculty of Medicine, University of PeradeniyaDepartment of Microbiology, Faculty of Medicine, The Chinese University of Hong KongAbstract Background Antibiotic Resistance is an imminent global public health threat. Antibiotic resistance emerged in healthcare settings and has now moved on to the community settings. This study was conducted to identify the rates of asymptomatic colonization with selected antibiotic resistant organisms, (Methicillin Resistant Staphylococcus aureus (MRSA), Extended Spectrum Beta Lactamase (ESBL) producing Escherichia coli and Klebsiella spp and carbapenem resistant E.coli and Klebsiella spp) - among a group of university students in Sri Lanka. Identification of genetic determinants of MRSA and ESBL was an additional objective of the study. Methods A self - collected nasal swab and a peri-rectal swab collected after passing stools were obtained. Routine microbiological methods were used for the isolation S.aureus from the nasal swab and E.coli and Klebsiella species from the peri-rectal swab. Antibiotic sensitivity testing was performed as recommended by clinical and laboratory standard institute (CLSI). Three (3) genes that are responsible for ESBL production; bla CTX-M, bla SHV, and bla TEM were tested using previously described primers and PCR procedures. Identification of MecA and PVL genes attributed to MRSA was also done with PCR. Results A total of 322 participants between 21 and 28 years were recruited representing 5 different faculties of study. Seventy one (22.0%) were colonized with S.aureus and 14 among them with MRSA, making the MRSA colonization rate of 4.3%. Forty five (15%) of the participants were colonized with an ESBL producing E.coli or Klebsiella spp. No one was colonized with carbapenem resistant E.coli or Klebsiella species. Of the 45 ESBL producers the commonest genetic determinant identified was bla CTX-M (n = 36), while 16 isolates had bla TEM and 7 had bla SHV. Similarly, of the 14 isolates identified as MRSA, 3 (21.4%) were found to be PVL positive while 11 (78.6%) were MecA positive. Conclusions A high rate of colonization with ESBL producing E.coli and Klebsiella species was noted in our study group.https://doi.org/10.1186/s12879-021-06289-zMRSAESBLCREColonizationUniversity students |
| spellingShingle | Thilini Munasinghe Gihani Vidanapathirana Shahlina Kuthubdeen Asela Ekanayake Sacheera Angulmaduwa Kunchana De Silva Susan Subhasinghe Ruwani Kalupahana Veranja Liyanapathirana Margaret Ip Colonization with selected antibiotic resistant bacteria among a cohort of Sri Lankan university students BMC Infectious Diseases MRSA ESBL CRE Colonization University students |
| title | Colonization with selected antibiotic resistant bacteria among a cohort of Sri Lankan university students |
| title_full | Colonization with selected antibiotic resistant bacteria among a cohort of Sri Lankan university students |
| title_fullStr | Colonization with selected antibiotic resistant bacteria among a cohort of Sri Lankan university students |
| title_full_unstemmed | Colonization with selected antibiotic resistant bacteria among a cohort of Sri Lankan university students |
| title_short | Colonization with selected antibiotic resistant bacteria among a cohort of Sri Lankan university students |
| title_sort | colonization with selected antibiotic resistant bacteria among a cohort of sri lankan university students |
| topic | MRSA ESBL CRE Colonization University students |
| url | https://doi.org/10.1186/s12879-021-06289-z |
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