Molecular Targets of Pinocembrin Underlying Its Regenerative Activities in Human Keratinocytes
Pinocembrin is one of the well-known compounds in the group of flavonoids. The pharmacological activities of pinocembrin in association with wound-healing activities have been reported. However, its effects on the aspect of cellular interaction underlying growth and survival are still unidentified i...
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MDPI AG
2022-07-01
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| Series: | Pharmaceuticals |
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| Online Access: | https://www.mdpi.com/1424-8247/15/8/954 |
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| author | Jirapak Ruttanapattanakul Nitwara Wikan Saranyapin Potikanond Wutigri Nimlamool |
| author_facet | Jirapak Ruttanapattanakul Nitwara Wikan Saranyapin Potikanond Wutigri Nimlamool |
| author_sort | Jirapak Ruttanapattanakul |
| collection | DOAJ |
| description | Pinocembrin is one of the well-known compounds in the group of flavonoids. The pharmacological activities of pinocembrin in association with wound-healing activities have been reported. However, its effects on the aspect of cellular interaction underlying growth and survival are still unidentified in human keratinocytes. Our previous study reported that <i>Boesenbergia rotunda</i> potently stimulated survival and proliferation of a human keratinocyte cell line (HaCaT). On the basis that pinocembrin is revealed to be one of the major constituents of this plant, we aimed to define the survival- and proliferation-enhancing effects of this compound at the cellular level. Results from the current study confirmed that pinocembrin induced an increase in HaCaT cell number. At the signaling perspective, we identified that pinocembrin significantly triggered ERK1/2 and Akt activation. The stimulating effects of pinocembrin were clearly inhibited by MEK and PI3K inhibitors authenticating that proliferation- and survival-promoting activities of pinocembrin were mainly acted on these two signaling cascades. Altogether, we successfully identified that pinocembrin functions to induce keratinocyte proliferation and survival, at least by provoking MAPK and PI3K pathways. Our study encourages the fact that pinocembrin is one of the interesting natural flavonoid compounds to be developed as a wound closure-promoting agent. |
| first_indexed | 2024-03-09T09:50:57Z |
| format | Article |
| id | doaj.art-6932f629a86549779fa5bb4302af8fed |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-09T09:50:57Z |
| publishDate | 2022-07-01 |
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| series | Pharmaceuticals |
| spelling | doaj.art-6932f629a86549779fa5bb4302af8fed2023-12-02T00:08:52ZengMDPI AGPharmaceuticals1424-82472022-07-0115895410.3390/ph15080954Molecular Targets of Pinocembrin Underlying Its Regenerative Activities in Human KeratinocytesJirapak Ruttanapattanakul0Nitwara Wikan1Saranyapin Potikanond2Wutigri Nimlamool3Department of Pharmacology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Pharmacology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Pharmacology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Pharmacology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, ThailandPinocembrin is one of the well-known compounds in the group of flavonoids. The pharmacological activities of pinocembrin in association with wound-healing activities have been reported. However, its effects on the aspect of cellular interaction underlying growth and survival are still unidentified in human keratinocytes. Our previous study reported that <i>Boesenbergia rotunda</i> potently stimulated survival and proliferation of a human keratinocyte cell line (HaCaT). On the basis that pinocembrin is revealed to be one of the major constituents of this plant, we aimed to define the survival- and proliferation-enhancing effects of this compound at the cellular level. Results from the current study confirmed that pinocembrin induced an increase in HaCaT cell number. At the signaling perspective, we identified that pinocembrin significantly triggered ERK1/2 and Akt activation. The stimulating effects of pinocembrin were clearly inhibited by MEK and PI3K inhibitors authenticating that proliferation- and survival-promoting activities of pinocembrin were mainly acted on these two signaling cascades. Altogether, we successfully identified that pinocembrin functions to induce keratinocyte proliferation and survival, at least by provoking MAPK and PI3K pathways. Our study encourages the fact that pinocembrin is one of the interesting natural flavonoid compounds to be developed as a wound closure-promoting agent.https://www.mdpi.com/1424-8247/15/8/954flavonoidspinocembrinwound healingkeratinocyteregenerative medicine |
| spellingShingle | Jirapak Ruttanapattanakul Nitwara Wikan Saranyapin Potikanond Wutigri Nimlamool Molecular Targets of Pinocembrin Underlying Its Regenerative Activities in Human Keratinocytes Pharmaceuticals flavonoids pinocembrin wound healing keratinocyte regenerative medicine |
| title | Molecular Targets of Pinocembrin Underlying Its Regenerative Activities in Human Keratinocytes |
| title_full | Molecular Targets of Pinocembrin Underlying Its Regenerative Activities in Human Keratinocytes |
| title_fullStr | Molecular Targets of Pinocembrin Underlying Its Regenerative Activities in Human Keratinocytes |
| title_full_unstemmed | Molecular Targets of Pinocembrin Underlying Its Regenerative Activities in Human Keratinocytes |
| title_short | Molecular Targets of Pinocembrin Underlying Its Regenerative Activities in Human Keratinocytes |
| title_sort | molecular targets of pinocembrin underlying its regenerative activities in human keratinocytes |
| topic | flavonoids pinocembrin wound healing keratinocyte regenerative medicine |
| url | https://www.mdpi.com/1424-8247/15/8/954 |
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