Study on Anticancer Activity of 4, 4'-[1,4-phenylenebis(1,3,4-thiadiazole-5,2-diyl)] bis(azaneylylidene) bis(methaneylylidene) diphenolon Breast Cancer Cells
New medicinal compounds are being evaluated due to the increasing prevalence of cancer in human societies and the necessity to produce new medications for treatment. The new Schiff base compound 4,4'-[1,4-phenylenebis(1,3,4-thiadiazole-5,2-diyl)] bis (azaneylylidene) bis (methaneylylidene) diph...
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| Format: | Article |
| Language: | English |
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Razi Vaccine and Serum Research Institute
2021-10-01
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| Series: | Archives of Razi Institute |
| Subjects: | |
| Online Access: | https://archrazi.areeo.ac.ir/article_124907_c41de80734ae1cf5b723cc9f98272f27.pdf |
| _version_ | 1819125753808158720 |
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| author | B. Saeed S. Al-Jadaan B. A Abbas |
| author_facet | B. Saeed S. Al-Jadaan B. A Abbas |
| author_sort | B. Saeed |
| collection | DOAJ |
| description | New medicinal compounds are being evaluated due to the increasing prevalence of cancer in human societies and the necessity to produce new medications for treatment. The new Schiff base compound 4,4'-[1,4-phenylenebis(1,3,4-thiadiazole-5,2-diyl)] bis (azaneylylidene) bis (methaneylylidene) diphenol, which was previously produced from the reaction of 5,5' [(1,4-Phenelene) bis (1,3,4-thiadiazol-2-amine)] and the para-hydroxy ben aldehyde was synthesized and different concentrations (250 and 300 mg/mL) of this new compound were exposed to breast cancer (MCF-7) cells to examine its cytotoxicity effect. Cell line viability, acridine orange/propidium iodide staining, and DNA fragmentation were assessed in evaluating the antitumor effect of the new composition. Obtained data from cell viability assays demonstrated cytotoxic activity against MCF-7 breast cancer cell lines. No fragmentation was observed in DNA fragmentation of the novel compound base with MCF-7 and Vero cell line. The new Schiff base compound indicated well-defined anti-cancer activity when treated with breast cancer cells (MCF-7). The compound blocked the proliferation of cancer cells without apoptosis. As a consequence of the findings, it was recommended to use this compound in treating breast cancer. |
| first_indexed | 2024-12-22T07:45:09Z |
| format | Article |
| id | doaj.art-69339ffdd7cd47b8a143977a97d81bf0 |
| institution | Directory Open Access Journal |
| issn | 0365-3439 2008-9872 |
| language | English |
| last_indexed | 2024-12-22T07:45:09Z |
| publishDate | 2021-10-01 |
| publisher | Razi Vaccine and Serum Research Institute |
| record_format | Article |
| series | Archives of Razi Institute |
| spelling | doaj.art-69339ffdd7cd47b8a143977a97d81bf02022-12-21T18:33:40ZengRazi Vaccine and Serum Research InstituteArchives of Razi Institute0365-34392008-98722021-10-0176482182710.22092/ari.2021.355941.1742124907Study on Anticancer Activity of 4, 4'-[1,4-phenylenebis(1,3,4-thiadiazole-5,2-diyl)] bis(azaneylylidene) bis(methaneylylidene) diphenolon Breast Cancer CellsB. Saeed0S. Al-Jadaan1B. A Abbas2University of Basrah, Al-Zahraa College of Medicine, Microbiology Department, Basrah, IraqUniversity of Basrah, College of Pharmacy, Pharmaceutical Chemistry Department, Basrah, IraqDepartment of Microbiology, College of Veterinary Medicine, University of Basrah, Basrah, IraqNew medicinal compounds are being evaluated due to the increasing prevalence of cancer in human societies and the necessity to produce new medications for treatment. The new Schiff base compound 4,4'-[1,4-phenylenebis(1,3,4-thiadiazole-5,2-diyl)] bis (azaneylylidene) bis (methaneylylidene) diphenol, which was previously produced from the reaction of 5,5' [(1,4-Phenelene) bis (1,3,4-thiadiazol-2-amine)] and the para-hydroxy ben aldehyde was synthesized and different concentrations (250 and 300 mg/mL) of this new compound were exposed to breast cancer (MCF-7) cells to examine its cytotoxicity effect. Cell line viability, acridine orange/propidium iodide staining, and DNA fragmentation were assessed in evaluating the antitumor effect of the new composition. Obtained data from cell viability assays demonstrated cytotoxic activity against MCF-7 breast cancer cell lines. No fragmentation was observed in DNA fragmentation of the novel compound base with MCF-7 and Vero cell line. The new Schiff base compound indicated well-defined anti-cancer activity when treated with breast cancer cells (MCF-7). The compound blocked the proliferation of cancer cells without apoptosis. As a consequence of the findings, it was recommended to use this compound in treating breast cancer.https://archrazi.areeo.ac.ir/article_124907_c41de80734ae1cf5b723cc9f98272f27.pdfbreast cancerdna laddering assaythiadiazole compound |
| spellingShingle | B. Saeed S. Al-Jadaan B. A Abbas Study on Anticancer Activity of 4, 4'-[1,4-phenylenebis(1,3,4-thiadiazole-5,2-diyl)] bis(azaneylylidene) bis(methaneylylidene) diphenolon Breast Cancer Cells Archives of Razi Institute breast cancer dna laddering assay thiadiazole compound |
| title | Study on Anticancer Activity of 4, 4'-[1,4-phenylenebis(1,3,4-thiadiazole-5,2-diyl)] bis(azaneylylidene) bis(methaneylylidene) diphenolon Breast Cancer Cells |
| title_full | Study on Anticancer Activity of 4, 4'-[1,4-phenylenebis(1,3,4-thiadiazole-5,2-diyl)] bis(azaneylylidene) bis(methaneylylidene) diphenolon Breast Cancer Cells |
| title_fullStr | Study on Anticancer Activity of 4, 4'-[1,4-phenylenebis(1,3,4-thiadiazole-5,2-diyl)] bis(azaneylylidene) bis(methaneylylidene) diphenolon Breast Cancer Cells |
| title_full_unstemmed | Study on Anticancer Activity of 4, 4'-[1,4-phenylenebis(1,3,4-thiadiazole-5,2-diyl)] bis(azaneylylidene) bis(methaneylylidene) diphenolon Breast Cancer Cells |
| title_short | Study on Anticancer Activity of 4, 4'-[1,4-phenylenebis(1,3,4-thiadiazole-5,2-diyl)] bis(azaneylylidene) bis(methaneylylidene) diphenolon Breast Cancer Cells |
| title_sort | study on anticancer activity of 4 4 1 4 phenylenebis 1 3 4 thiadiazole 5 2 diyl bis azaneylylidene bis methaneylylidene diphenolon breast cancer cells |
| topic | breast cancer dna laddering assay thiadiazole compound |
| url | https://archrazi.areeo.ac.ir/article_124907_c41de80734ae1cf5b723cc9f98272f27.pdf |
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