In vitro assessment of genotoxic and cytotoxic effects of Artemisia annua L. tincture
The genus Artemisia (fam. Asteraceae) is one of the largest and widely distributed with around 500 species, majority used as aromatic and medicinal plants. Artemisia annua L. is widely used as a dietary spice, herbal tea, as a supplement, and in a non-pharmaceutical form for treatment of malari...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
University of Sarajevo, Institute for Genetic Engineering and Biotechnology
2021-12-01
|
| Series: | Genetics & Applications |
| Subjects: | |
| Online Access: | https://genapp.ba/editions/index.php/journal/article/view/142 |
| _version_ | 1811311610130595840 |
|---|---|
| author | Tamara Cetkovic Anja Haveric Lejla Caluk Klacar Maida Hadzic Omanovic Sanin Haveric |
| author_facet | Tamara Cetkovic Anja Haveric Lejla Caluk Klacar Maida Hadzic Omanovic Sanin Haveric |
| author_sort | Tamara Cetkovic |
| collection | DOAJ |
| description |
The genus Artemisia (fam. Asteraceae) is one of the largest and widely distributed with around 500 species, majority used as aromatic and medicinal plants. Artemisia annua L. is widely used as a dietary spice, herbal tea, as a supplement, and in a non-pharmaceutical form for treatment of malaria and fever. It is orally consumed as capsules, extracts and tinctures and topically applied as an essential oil diluted in lotions and ointments. Artemisinin is the main constituent of Artemisia annua L. extracts. Since the discovery that the artemisinin is efficient in malaria treatment, there is also a growth in consumption of A. annua extracts for antitumour and even recently for antiviral treatments against SARS-CoV-2 infections. This study aimed to investigate genotoxic effect in peripheral blood culture and cytotoxic effects in cancer and normal cell lines, of commercially available A. annua L. tincture in series of dilutions. Both comet and neutral red uptake assays revealed dose-dependent genotoxicity and cytotoxicity of A. annua tincture dilutions. Comet assay revealed significantly increased DNA damage in peripheral blood cells while neutral-red assays showed increase in cytotoxicity (p<0.001) in both normal and cancer cell cultures treated with the lowest extract dilution compared to the highest one applied. Obtained results indicate caution needed in A. annua L. tincture use, especially when poorly diluted.
|
| first_indexed | 2024-04-13T10:20:39Z |
| format | Article |
| id | doaj.art-693535b805f846f98703606a43813d1d |
| institution | Directory Open Access Journal |
| issn | 2566-2937 2566-431X |
| language | English |
| last_indexed | 2024-04-13T10:20:39Z |
| publishDate | 2021-12-01 |
| publisher | University of Sarajevo, Institute for Genetic Engineering and Biotechnology |
| record_format | Article |
| series | Genetics & Applications |
| spelling | doaj.art-693535b805f846f98703606a43813d1d2022-12-22T02:50:29ZengUniversity of Sarajevo, Institute for Genetic Engineering and BiotechnologyGenetics & Applications2566-29372566-431X2021-12-015210.31383/ga.vol5iss2pp1-9In vitro assessment of genotoxic and cytotoxic effects of Artemisia annua L. tinctureTamara Cetkovic0Anja Haveric1Lejla Caluk Klacar2Maida Hadzic Omanovic3Sanin Haveric4University of Sarajevo-Institute for Genetic Engineering and Biotechnology, Sarajevo, Bosnia and HerzegovinaUniversity of Sarajevo-Institute for Genetic Engineering and Biotechnology, Sarajevo, Bosnia and HerzegovinaUniversity of Sarajevo-Institute for Genetic Engineering and Biotechnology, Sarajevo, Bosnia and HerzegovinaUniversity of Sarajevo-Institute for Genetic Engineering and Biotechnology, Sarajevo, Bosnia and HerzegovinaUniversity of Sarajevo-Institute for Genetic Engineering and Biotechnology, Sarajevo, Bosnia and Herzegovina The genus Artemisia (fam. Asteraceae) is one of the largest and widely distributed with around 500 species, majority used as aromatic and medicinal plants. Artemisia annua L. is widely used as a dietary spice, herbal tea, as a supplement, and in a non-pharmaceutical form for treatment of malaria and fever. It is orally consumed as capsules, extracts and tinctures and topically applied as an essential oil diluted in lotions and ointments. Artemisinin is the main constituent of Artemisia annua L. extracts. Since the discovery that the artemisinin is efficient in malaria treatment, there is also a growth in consumption of A. annua extracts for antitumour and even recently for antiviral treatments against SARS-CoV-2 infections. This study aimed to investigate genotoxic effect in peripheral blood culture and cytotoxic effects in cancer and normal cell lines, of commercially available A. annua L. tincture in series of dilutions. Both comet and neutral red uptake assays revealed dose-dependent genotoxicity and cytotoxicity of A. annua tincture dilutions. Comet assay revealed significantly increased DNA damage in peripheral blood cells while neutral-red assays showed increase in cytotoxicity (p<0.001) in both normal and cancer cell cultures treated with the lowest extract dilution compared to the highest one applied. Obtained results indicate caution needed in A. annua L. tincture use, especially when poorly diluted. https://genapp.ba/editions/index.php/journal/article/view/142comet assayneutral red uptake assaymedicinal plantscancer cell lines |
| spellingShingle | Tamara Cetkovic Anja Haveric Lejla Caluk Klacar Maida Hadzic Omanovic Sanin Haveric In vitro assessment of genotoxic and cytotoxic effects of Artemisia annua L. tincture Genetics & Applications comet assay neutral red uptake assay medicinal plants cancer cell lines |
| title | In vitro assessment of genotoxic and cytotoxic effects of Artemisia annua L. tincture |
| title_full | In vitro assessment of genotoxic and cytotoxic effects of Artemisia annua L. tincture |
| title_fullStr | In vitro assessment of genotoxic and cytotoxic effects of Artemisia annua L. tincture |
| title_full_unstemmed | In vitro assessment of genotoxic and cytotoxic effects of Artemisia annua L. tincture |
| title_short | In vitro assessment of genotoxic and cytotoxic effects of Artemisia annua L. tincture |
| title_sort | in vitro assessment of genotoxic and cytotoxic effects of artemisia annua l tincture |
| topic | comet assay neutral red uptake assay medicinal plants cancer cell lines |
| url | https://genapp.ba/editions/index.php/journal/article/view/142 |
| work_keys_str_mv | AT tamaracetkovic invitroassessmentofgenotoxicandcytotoxiceffectsofartemisiaannualtincture AT anjahaveric invitroassessmentofgenotoxicandcytotoxiceffectsofartemisiaannualtincture AT lejlacalukklacar invitroassessmentofgenotoxicandcytotoxiceffectsofartemisiaannualtincture AT maidahadzicomanovic invitroassessmentofgenotoxicandcytotoxiceffectsofartemisiaannualtincture AT saninhaveric invitroassessmentofgenotoxicandcytotoxiceffectsofartemisiaannualtincture |