CRISPR technology: A versatile tool to model, screen, and reverse drug resistance in cancer
Background: Drug resistance is a serious challenge in cancer treatment that can render chemotherapy a failure. Understanding the mechanisms behind drug resistance and developing novel therapeutic approaches are cardinal steps in overcoming this issue. Clustered regularly interspaced short palindrome...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | European Journal of Cell Biology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0171933523000146 |
| _version_ | 1827920706664398848 |
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| author | Shiva Shirani-Bidabadi Aliye Tabatabaee Nazita Tavazohi Amirali Hariri Amir Reza Aref Ali Zarrabi Nicolette Casarcia Anupam Bishayee Mina Mirian |
| author_facet | Shiva Shirani-Bidabadi Aliye Tabatabaee Nazita Tavazohi Amirali Hariri Amir Reza Aref Ali Zarrabi Nicolette Casarcia Anupam Bishayee Mina Mirian |
| author_sort | Shiva Shirani-Bidabadi |
| collection | DOAJ |
| description | Background: Drug resistance is a serious challenge in cancer treatment that can render chemotherapy a failure. Understanding the mechanisms behind drug resistance and developing novel therapeutic approaches are cardinal steps in overcoming this issue. Clustered regularly interspaced short palindrome repeats (CRISPR) gene-editing technology has proven to be a useful tool to study cancer drug resistance mechanisms and target the responsible genes. In this review, we evaluated original research studies that used the CRISPR tool in three areas related to drug resistance, namely screening resistance-related genes, generating modified models of resistant cells and animals, and removing resistance by genetic manipulation. We reported the targeted genes, study models, and drug groups in these studies. In addition to discussing different applications of CRISPR technology in cancer drug resistance, we analyzed drug resistance mechanisms and provided examples of CRISPR’s role in studying them. Although CRISPR is a powerful tool for examining drug resistance and sensitizing resistant cells to chemotherapy, more studies are required to overcome its disadvantages, such as off-target effects, immunotoxicity, and inefficient delivery of CRISPR/cas9 into the cells. |
| first_indexed | 2024-03-13T04:14:39Z |
| format | Article |
| id | doaj.art-6935db44b79844ba9d38dfad3b57595c |
| institution | Directory Open Access Journal |
| issn | 0171-9335 |
| language | English |
| last_indexed | 2024-03-13T04:14:39Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | European Journal of Cell Biology |
| spelling | doaj.art-6935db44b79844ba9d38dfad3b57595c2023-06-21T06:50:54ZengElsevierEuropean Journal of Cell Biology0171-93352023-06-011022151299CRISPR technology: A versatile tool to model, screen, and reverse drug resistance in cancerShiva Shirani-Bidabadi0Aliye Tabatabaee1Nazita Tavazohi2Amirali Hariri3Amir Reza Aref4Ali Zarrabi5Nicolette Casarcia6Anupam Bishayee7Mina Mirian8Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Isfahan University of Medical Sciences, Isfahan 8174673461, IranFaculty of Pharmacy, Isfahan University of Medical Sciences, Isfahan 8174673461, IranNovel Drug Delivery Systems Research Centre, Faculty of Pharmacy, Isfahan University of Medical Sciences, Isfahan 8174673461, IranDepartment of Pharmaceutical Biotechnology, Faculty of Pharmacy, Isfahan University of Medical Sciences, Isfahan 8174673461, IranBelfer Center for Applied Cancer Science, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02215, USA; Translational Sciences, Xsphera Biosciences Inc., Boston, MA 02215, USADepartment of Biomedical Engineering, Faculty of Engineering and Natural Sciences, Istinye University, Istanbul 34396, TurkeyCollege of Osteopathic Medicine, Lake Erie College of Osteopathic Medicine, Bradenton, FL 34211, USACollege of Osteopathic Medicine, Lake Erie College of Osteopathic Medicine, Bradenton, FL 34211, USA; Correspondence to: College of Osteopathic Medicine, Lake Erie College of Osteopathic Medicine, 5000 Lakewood Ranch Boulevard, Bradenton, FL 34211, USA.Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Isfahan University of Medical Sciences, Isfahan 8174673461, Iran; Corresponding author.Background: Drug resistance is a serious challenge in cancer treatment that can render chemotherapy a failure. Understanding the mechanisms behind drug resistance and developing novel therapeutic approaches are cardinal steps in overcoming this issue. Clustered regularly interspaced short palindrome repeats (CRISPR) gene-editing technology has proven to be a useful tool to study cancer drug resistance mechanisms and target the responsible genes. In this review, we evaluated original research studies that used the CRISPR tool in three areas related to drug resistance, namely screening resistance-related genes, generating modified models of resistant cells and animals, and removing resistance by genetic manipulation. We reported the targeted genes, study models, and drug groups in these studies. In addition to discussing different applications of CRISPR technology in cancer drug resistance, we analyzed drug resistance mechanisms and provided examples of CRISPR’s role in studying them. Although CRISPR is a powerful tool for examining drug resistance and sensitizing resistant cells to chemotherapy, more studies are required to overcome its disadvantages, such as off-target effects, immunotoxicity, and inefficient delivery of CRISPR/cas9 into the cells.http://www.sciencedirect.com/science/article/pii/S0171933523000146CancerDrug resistanceCRISPR technologyCancer therapy |
| spellingShingle | Shiva Shirani-Bidabadi Aliye Tabatabaee Nazita Tavazohi Amirali Hariri Amir Reza Aref Ali Zarrabi Nicolette Casarcia Anupam Bishayee Mina Mirian CRISPR technology: A versatile tool to model, screen, and reverse drug resistance in cancer European Journal of Cell Biology Cancer Drug resistance CRISPR technology Cancer therapy |
| title | CRISPR technology: A versatile tool to model, screen, and reverse drug resistance in cancer |
| title_full | CRISPR technology: A versatile tool to model, screen, and reverse drug resistance in cancer |
| title_fullStr | CRISPR technology: A versatile tool to model, screen, and reverse drug resistance in cancer |
| title_full_unstemmed | CRISPR technology: A versatile tool to model, screen, and reverse drug resistance in cancer |
| title_short | CRISPR technology: A versatile tool to model, screen, and reverse drug resistance in cancer |
| title_sort | crispr technology a versatile tool to model screen and reverse drug resistance in cancer |
| topic | Cancer Drug resistance CRISPR technology Cancer therapy |
| url | http://www.sciencedirect.com/science/article/pii/S0171933523000146 |
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