Complete genome sequence of the multidrug-resistant clinical isolate Acinetobacter baumannii KBN10P05679: Insights into antimicrobial resistance genotype and potential virulence traits
ABSTRACT: Objectives: Acinetobacter baumannii, a nosocomial pathogen, exhibits multidrug resistance and is a major concern worldwide. We therefore aimed to evaluate the genomic features of the clinical strain A. baumannii KBN10P05679 to elucidate its antibiotic resistance mechanisms and virulence f...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | Journal of Global Antimicrobial Resistance |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716523000322 |
| _version_ | 1797802241039532032 |
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| author | Md. Maidul Islam Jeongah Kim Kyeongmin Kim Dooyoung Kim Samia Akter Seunghyeok Bang Shukho Kim Jungmin Kim Je Chul Lee Minsang Shin |
| author_facet | Md. Maidul Islam Jeongah Kim Kyeongmin Kim Dooyoung Kim Samia Akter Seunghyeok Bang Shukho Kim Jungmin Kim Je Chul Lee Minsang Shin |
| author_sort | Md. Maidul Islam |
| collection | DOAJ |
| description | ABSTRACT: Objectives: Acinetobacter baumannii, a nosocomial pathogen, exhibits multidrug resistance and is a major concern worldwide. We therefore aimed to evaluate the genomic features of the clinical strain A. baumannii KBN10P05679 to elucidate its antibiotic resistance mechanisms and virulence factors. Methods: In silico multilocus sequence typing, phylogenetic identification, genome annotation, genome analysis, antibiotic susceptibility testing, and biofilm formation assay were performed, and the expression levels of antibiotic resistance- and biofilm-related genes were investigated. Results: The complete genome of KBN10P05679 comprises a circular chromosome of 3 990 428 bp and two plasmids (74 294 and 8731 bp) and was assigned to the ST451 sequence type. Clusters of Orthologous Gene annotation identified 3810 genes, including those involved in amino acid transport and metabolism, transcription, inorganic ion transport, energy production and conversion, replication, recombination and repair, and carbohydrate and protein metabolism. The antibiotic resistance genes were investigated by searching the Comprehensive Antibiotic Resistance Database, and the genome was found to harbour 30 different antibiotic resistance genes. Analysis of the Virulence Factor Database revealed 86 virulence factor genes in the KBN1005679 genome. The KBN10P05679 strain was found to have a higher capacity for biofilm formation and expressed biofilm-related genes at a higher level than the other tested strains. Conclusions: The antibiotic resistance genotype and potential virulence factor-related data obtained in this study would help direct future studies for developing the control measures for this multidrug-resistant pathogen. |
| first_indexed | 2024-03-13T05:03:01Z |
| format | Article |
| id | doaj.art-69364b0032de484385157b76f61d2a3e |
| institution | Directory Open Access Journal |
| issn | 2213-7165 |
| language | English |
| last_indexed | 2024-03-13T05:03:01Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj.art-69364b0032de484385157b76f61d2a3e2023-06-17T05:18:26ZengElsevierJournal of Global Antimicrobial Resistance2213-71652023-06-01337882Complete genome sequence of the multidrug-resistant clinical isolate Acinetobacter baumannii KBN10P05679: Insights into antimicrobial resistance genotype and potential virulence traitsMd. Maidul Islam0Jeongah Kim1Kyeongmin Kim2Dooyoung Kim3Samia Akter4Seunghyeok Bang5Shukho Kim6Jungmin Kim7Je Chul Lee8Minsang Shin9Department of Microbiology, School of Medicine, Kyungpook National University, Daegu, South KoreaDepartment of Microbiology, School of Medicine, Kyungpook National University, Daegu, South KoreaDepartment of Microbiology, School of Medicine, Kyungpook National University, Daegu, South KoreaDepartment of Microbiology, School of Medicine, Kyungpook National University, Daegu, South KoreaDepartment of Microbiology, School of Medicine, Kyungpook National University, Daegu, South KoreaDepartment of Microbiology, School of Medicine, Kyungpook National University, Daegu, South KoreaDepartment of Microbiology, School of Medicine, Kyungpook National University, Daegu, South KoreaDepartment of Microbiology, School of Medicine, Kyungpook National University, Daegu, South KoreaDepartment of Microbiology, School of Medicine, Kyungpook National University, Daegu, South KoreaCorresponding author. Department of Microbiology, School of Medicine, Kyungpook National University, 680 Gukchaebosang-Ro, Jung-gu, Daegu 41944, South Korea.; Department of Microbiology, School of Medicine, Kyungpook National University, Daegu, South KoreaABSTRACT: Objectives: Acinetobacter baumannii, a nosocomial pathogen, exhibits multidrug resistance and is a major concern worldwide. We therefore aimed to evaluate the genomic features of the clinical strain A. baumannii KBN10P05679 to elucidate its antibiotic resistance mechanisms and virulence factors. Methods: In silico multilocus sequence typing, phylogenetic identification, genome annotation, genome analysis, antibiotic susceptibility testing, and biofilm formation assay were performed, and the expression levels of antibiotic resistance- and biofilm-related genes were investigated. Results: The complete genome of KBN10P05679 comprises a circular chromosome of 3 990 428 bp and two plasmids (74 294 and 8731 bp) and was assigned to the ST451 sequence type. Clusters of Orthologous Gene annotation identified 3810 genes, including those involved in amino acid transport and metabolism, transcription, inorganic ion transport, energy production and conversion, replication, recombination and repair, and carbohydrate and protein metabolism. The antibiotic resistance genes were investigated by searching the Comprehensive Antibiotic Resistance Database, and the genome was found to harbour 30 different antibiotic resistance genes. Analysis of the Virulence Factor Database revealed 86 virulence factor genes in the KBN1005679 genome. The KBN10P05679 strain was found to have a higher capacity for biofilm formation and expressed biofilm-related genes at a higher level than the other tested strains. Conclusions: The antibiotic resistance genotype and potential virulence factor-related data obtained in this study would help direct future studies for developing the control measures for this multidrug-resistant pathogen.http://www.sciencedirect.com/science/article/pii/S2213716523000322Acinetobacter baumannii KBN10P05679Whole-genome sequencing (WGS)Antibiotic resistanceBiofilm formationVirulence |
| spellingShingle | Md. Maidul Islam Jeongah Kim Kyeongmin Kim Dooyoung Kim Samia Akter Seunghyeok Bang Shukho Kim Jungmin Kim Je Chul Lee Minsang Shin Complete genome sequence of the multidrug-resistant clinical isolate Acinetobacter baumannii KBN10P05679: Insights into antimicrobial resistance genotype and potential virulence traits Journal of Global Antimicrobial Resistance Acinetobacter baumannii KBN10P05679 Whole-genome sequencing (WGS) Antibiotic resistance Biofilm formation Virulence |
| title | Complete genome sequence of the multidrug-resistant clinical isolate Acinetobacter baumannii KBN10P05679: Insights into antimicrobial resistance genotype and potential virulence traits |
| title_full | Complete genome sequence of the multidrug-resistant clinical isolate Acinetobacter baumannii KBN10P05679: Insights into antimicrobial resistance genotype and potential virulence traits |
| title_fullStr | Complete genome sequence of the multidrug-resistant clinical isolate Acinetobacter baumannii KBN10P05679: Insights into antimicrobial resistance genotype and potential virulence traits |
| title_full_unstemmed | Complete genome sequence of the multidrug-resistant clinical isolate Acinetobacter baumannii KBN10P05679: Insights into antimicrobial resistance genotype and potential virulence traits |
| title_short | Complete genome sequence of the multidrug-resistant clinical isolate Acinetobacter baumannii KBN10P05679: Insights into antimicrobial resistance genotype and potential virulence traits |
| title_sort | complete genome sequence of the multidrug resistant clinical isolate acinetobacter baumannii kbn10p05679 insights into antimicrobial resistance genotype and potential virulence traits |
| topic | Acinetobacter baumannii KBN10P05679 Whole-genome sequencing (WGS) Antibiotic resistance Biofilm formation Virulence |
| url | http://www.sciencedirect.com/science/article/pii/S2213716523000322 |
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