Genomic analyses of two novel biofilm-degrading methicillin-resistant Staphylococcus aureus phages
Abstract Background Methicillin-resistant Staphylococcus aureus (MRSA) biofilm producers represent an important etiological agent of many chronic human infections. Antibiotics and host immune responses are largely ineffective against bacteria within biofilms. Alternative actions and novel antimicrob...
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| Format: | Article |
| Language: | English |
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BMC
2019-05-01
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| Series: | BMC Microbiology |
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| Online Access: | http://link.springer.com/article/10.1186/s12866-019-1484-9 |
| _version_ | 1818776657961418752 |
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| author | Khulood Hamid Dakheel Raha Abdul Rahim Vasantha Kumari Neela Jameel R. Al-Obaidi Tan Geok Hun Mohd Noor Mat Isa Khatijah Yusoff |
| author_facet | Khulood Hamid Dakheel Raha Abdul Rahim Vasantha Kumari Neela Jameel R. Al-Obaidi Tan Geok Hun Mohd Noor Mat Isa Khatijah Yusoff |
| author_sort | Khulood Hamid Dakheel |
| collection | DOAJ |
| description | Abstract Background Methicillin-resistant Staphylococcus aureus (MRSA) biofilm producers represent an important etiological agent of many chronic human infections. Antibiotics and host immune responses are largely ineffective against bacteria within biofilms. Alternative actions and novel antimicrobials should be considered. In this context, the use of phages to destroy MRSA biofilms presents an innovative alternative mechanism. Results Twenty-five MRSA biofilm producers were used as substrates to isolate MRSA-specific phages. Despite the difficulties in obtaining an isolate of this phage, two phages (UPMK_1 and UPMK_2) were isolated. Both phages varied in their ability to produce halos around their plaques, host infectivity, one-step growth curves, and electron microscopy features. Furthermore, both phages demonstrated antagonistic infectivity on planktonic cultures. This was validated in an in vitro static biofilm assay (in microtiter-plates), followed by the visualization of the biofilm architecture in situ via confocal laser scanning microscopy before and after phage infection, and further supported by phages genome analysis. The UPMK_1 genome comprised 152,788 bp coding for 155 putative open reading frames (ORFs), and its genome characteristics were between the Myoviridae and Siphoviridae family, though the morphological features confined it more to the Siphoviridae family. The UPMK_2 has 40,955 bp with 62 putative ORFs; morphologically, it presented the features of the Podoviridae though its genome did not show similarity with any of the S. aureus in the Podoviridae family. Both phages possess lytic enzymes that were associated with a high ability to degrade biofilms as shown in the microtiter plate and CLSM analyses. Conclusions The present work addressed the possibility of using phages as potential biocontrol agents for biofilm-producing MRSA. |
| first_indexed | 2024-12-18T11:16:25Z |
| format | Article |
| id | doaj.art-69397f70df0e4a5f9b466b45c7ca4080 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-18T11:16:25Z |
| publishDate | 2019-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-69397f70df0e4a5f9b466b45c7ca40802022-12-21T21:09:55ZengBMCBMC Microbiology1471-21802019-05-0119112310.1186/s12866-019-1484-9Genomic analyses of two novel biofilm-degrading methicillin-resistant Staphylococcus aureus phagesKhulood Hamid Dakheel0Raha Abdul Rahim1Vasantha Kumari Neela2Jameel R. Al-Obaidi3Tan Geok Hun4Mohd Noor Mat Isa5Khatijah Yusoff6Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra MalaysiaDepartment of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra MalaysiaDepartment of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra MalaysiaAgro-biotechnology Institute Malaysia (ABI), c/o MARDI HeadquartersDepartment of Agriculture Technology, Faculty of Agriculture, Universiti Putra MalaysiaMalaysia Genome Institute (MGI)Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra MalaysiaAbstract Background Methicillin-resistant Staphylococcus aureus (MRSA) biofilm producers represent an important etiological agent of many chronic human infections. Antibiotics and host immune responses are largely ineffective against bacteria within biofilms. Alternative actions and novel antimicrobials should be considered. In this context, the use of phages to destroy MRSA biofilms presents an innovative alternative mechanism. Results Twenty-five MRSA biofilm producers were used as substrates to isolate MRSA-specific phages. Despite the difficulties in obtaining an isolate of this phage, two phages (UPMK_1 and UPMK_2) were isolated. Both phages varied in their ability to produce halos around their plaques, host infectivity, one-step growth curves, and electron microscopy features. Furthermore, both phages demonstrated antagonistic infectivity on planktonic cultures. This was validated in an in vitro static biofilm assay (in microtiter-plates), followed by the visualization of the biofilm architecture in situ via confocal laser scanning microscopy before and after phage infection, and further supported by phages genome analysis. The UPMK_1 genome comprised 152,788 bp coding for 155 putative open reading frames (ORFs), and its genome characteristics were between the Myoviridae and Siphoviridae family, though the morphological features confined it more to the Siphoviridae family. The UPMK_2 has 40,955 bp with 62 putative ORFs; morphologically, it presented the features of the Podoviridae though its genome did not show similarity with any of the S. aureus in the Podoviridae family. Both phages possess lytic enzymes that were associated with a high ability to degrade biofilms as shown in the microtiter plate and CLSM analyses. Conclusions The present work addressed the possibility of using phages as potential biocontrol agents for biofilm-producing MRSA.http://link.springer.com/article/10.1186/s12866-019-1484-9MRSA biofilmBacteriophageVirusConfocal laser scanning microscopy (CLSM)Microtiter plates |
| spellingShingle | Khulood Hamid Dakheel Raha Abdul Rahim Vasantha Kumari Neela Jameel R. Al-Obaidi Tan Geok Hun Mohd Noor Mat Isa Khatijah Yusoff Genomic analyses of two novel biofilm-degrading methicillin-resistant Staphylococcus aureus phages BMC Microbiology MRSA biofilm Bacteriophage Virus Confocal laser scanning microscopy (CLSM) Microtiter plates |
| title | Genomic analyses of two novel biofilm-degrading methicillin-resistant Staphylococcus aureus phages |
| title_full | Genomic analyses of two novel biofilm-degrading methicillin-resistant Staphylococcus aureus phages |
| title_fullStr | Genomic analyses of two novel biofilm-degrading methicillin-resistant Staphylococcus aureus phages |
| title_full_unstemmed | Genomic analyses of two novel biofilm-degrading methicillin-resistant Staphylococcus aureus phages |
| title_short | Genomic analyses of two novel biofilm-degrading methicillin-resistant Staphylococcus aureus phages |
| title_sort | genomic analyses of two novel biofilm degrading methicillin resistant staphylococcus aureus phages |
| topic | MRSA biofilm Bacteriophage Virus Confocal laser scanning microscopy (CLSM) Microtiter plates |
| url | http://link.springer.com/article/10.1186/s12866-019-1484-9 |
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