Bioremediation of lead contaminated environment by Bacillus cereus strain BUK_BCH_BTE2: Isolation and characterization of the bacterium
Lead is one of the most toxic heavy metals ever known by man today, which has zero biological role. It is known to cause anemia and affect the nervous and reproductive systems with irreversible effects. Its continual usage leads to its high accumulation polluting the soil and water bodies with devas...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Case Studies in Chemical and Environmental Engineering |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666016423002451 |
| _version_ | 1797447990956261376 |
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| author | Fatima Abdullahi Harun Muhammad Rabiú Yusuf Shehu Usman Dayyabu Shehu Kamaluddeen Babagana Aminu Jibril Sufyanu Muhammad Mustapha Jibril Aliyu Maje Bello Kabiru Abubakar Musa Ahmad Hussaini Jagaba Mohd Yunus Shukor Hafeez Muhammad Yakasai |
| author_facet | Fatima Abdullahi Harun Muhammad Rabiú Yusuf Shehu Usman Dayyabu Shehu Kamaluddeen Babagana Aminu Jibril Sufyanu Muhammad Mustapha Jibril Aliyu Maje Bello Kabiru Abubakar Musa Ahmad Hussaini Jagaba Mohd Yunus Shukor Hafeez Muhammad Yakasai |
| author_sort | Fatima Abdullahi Harun |
| collection | DOAJ |
| description | Lead is one of the most toxic heavy metals ever known by man today, which has zero biological role. It is known to cause anemia and affect the nervous and reproductive systems with irreversible effects. Its continual usage leads to its high accumulation polluting the soil and water bodies with devastating health effects. This research, aimed at isolation and characterization of indigenous lead tolerant bacteria from contaminated soil of Anka. Results of Atomic Absorption Spectrophotometry (AAS) showed high concentration of lead (738 mg/kg) of the affected sites above the EPA standard (400 mg/kg). Similarly, a mineral salt media was used to isolate the bacteria following serial dilution. An indigenous bacterial isolate (Ac) with potential to tolerate up to 3000 mg/L Pb(NO3)2 was isolated and molecularly identified based on 16s rRNA sequencing as Bacillus cereus strain BUK_BCH_BTE2 with the accession number MT160412. The isolate was further characterized for its optimum growth and tolerance conditions using one factor at a time (OFAT). Sucrose with an optimum concentration of 5 g/L was the best carbon source for the isolate. The isolate was found to utilize ammonium sulphate as the best nitrogen source at a concentration of 2.5 g/L, with an optimum pH and tempertaure of 7.0 and 37 °C respectively. A concentration of 1000 mg/L Pb(NO3)2 was found to be the optimum concentration for the isolate. The optimum incubation time and inoculum size were found to be 48 hrs and 100 μL respectively. The fact that the isolate could tolerate high Pb(NO3)2 concentration makes it suitable for future bioremediation work involving lead. |
| first_indexed | 2024-03-09T14:03:55Z |
| format | Article |
| id | doaj.art-693c0aa8ad294447be5e8259262b27f6 |
| institution | Directory Open Access Journal |
| issn | 2666-0164 |
| language | English |
| last_indexed | 2024-03-09T14:03:55Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Case Studies in Chemical and Environmental Engineering |
| spelling | doaj.art-693c0aa8ad294447be5e8259262b27f62023-11-30T05:11:24ZengElsevierCase Studies in Chemical and Environmental Engineering2666-01642023-12-018100540Bioremediation of lead contaminated environment by Bacillus cereus strain BUK_BCH_BTE2: Isolation and characterization of the bacteriumFatima Abdullahi Harun0Muhammad Rabiú Yusuf1Shehu Usman2Dayyabu Shehu3Kamaluddeen Babagana4Aminu Jibril Sufyanu5Muhammad Mustapha Jibril6Aliyu Maje Bello7Kabiru Abubakar Musa8Ahmad Hussaini Jagaba9Mohd Yunus Shukor10Hafeez Muhammad Yakasai11Department of Biochemistry, Faculty of Basic Medical Sciences, College of Health Sciences, Bayero University Kano, P.M.B. 3011, Kano, Nigeria; Department of Biochemistry, Faculty of Science and Computing, Capital City University Kano, P.M.B. 3409, Kano, NigeriaDepartment of Biochemistry, Faculty of Basic Medical Sciences, College of Health Sciences, Bayero University Kano, P.M.B. 3011, Kano, NigeriaDepartment of Plant Science and Biotechnology, Federal University Dutsin-Ma, Katsina, NigeriaDepartment of Biochemistry, Faculty of Basic Medical Sciences, College of Health Sciences, Bayero University Kano, P.M.B. 3011, Kano, NigeriaDepartment of Biochemistry, Faculty of Basic Medical Sciences, College of Health Sciences, Bayero University Kano, P.M.B. 3011, Kano, NigeriaDepartment of Biochemistry, Faculty of Basic Medical Sciences, College of Health Sciences, Bayero University Kano, P.M.B. 3011, Kano, NigeriaDepartment of Biochemistry, Faculty of Basic Medical Sciences, College of Health Sciences, Bayero University Kano, P.M.B. 3011, Kano, NigeriaDepartment of Biochemistry, Faculty of Basic Medical Sciences, College of Health Sciences, Bayero University Kano, P.M.B. 3011, Kano, NigeriaDepartment of Biochemistry, Faculty of Basic Medical Sciences, College of Health Sciences, Bayero University Kano, P.M.B. 3011, Kano, NigeriaInterdisciplinary Research Centre for Membranes and Water Security, King Fahd University of Petroleum and Minerals, Dhahran, 31261, Saudi Arabia; Corresponding author. Interdisciplinary Research Centre for Membranes and Water Security, King Fahd University of Petroleum and Minerals, Dhahran, 31261, Saudi Arabia.Department of Biochemistry, Faculty of Biotechnology and Biomolecular Science, Universiti Putra Malaysia, 43400, Serdang Selangor, Darul Ehsan, MalaysiaDepartment of Biochemistry, Faculty of Basic Medical Sciences, College of Health Sciences, Bayero University Kano, P.M.B. 3011, Kano, Nigeria; Skyline University Nigeria, No. 2 Zaria Road, Kano State, Nigeria; Corresponding author. Department of Biochemistry, Faculty of Basic Medical Sciences, College of Health Sciences, Bayero University Kano, P.M.B. 3011, Kano, Nigeria.Lead is one of the most toxic heavy metals ever known by man today, which has zero biological role. It is known to cause anemia and affect the nervous and reproductive systems with irreversible effects. Its continual usage leads to its high accumulation polluting the soil and water bodies with devastating health effects. This research, aimed at isolation and characterization of indigenous lead tolerant bacteria from contaminated soil of Anka. Results of Atomic Absorption Spectrophotometry (AAS) showed high concentration of lead (738 mg/kg) of the affected sites above the EPA standard (400 mg/kg). Similarly, a mineral salt media was used to isolate the bacteria following serial dilution. An indigenous bacterial isolate (Ac) with potential to tolerate up to 3000 mg/L Pb(NO3)2 was isolated and molecularly identified based on 16s rRNA sequencing as Bacillus cereus strain BUK_BCH_BTE2 with the accession number MT160412. The isolate was further characterized for its optimum growth and tolerance conditions using one factor at a time (OFAT). Sucrose with an optimum concentration of 5 g/L was the best carbon source for the isolate. The isolate was found to utilize ammonium sulphate as the best nitrogen source at a concentration of 2.5 g/L, with an optimum pH and tempertaure of 7.0 and 37 °C respectively. A concentration of 1000 mg/L Pb(NO3)2 was found to be the optimum concentration for the isolate. The optimum incubation time and inoculum size were found to be 48 hrs and 100 μL respectively. The fact that the isolate could tolerate high Pb(NO3)2 concentration makes it suitable for future bioremediation work involving lead.http://www.sciencedirect.com/science/article/pii/S2666016423002451LeadHeavy metalsIsolationBacteriaLead-tolerant |
| spellingShingle | Fatima Abdullahi Harun Muhammad Rabiú Yusuf Shehu Usman Dayyabu Shehu Kamaluddeen Babagana Aminu Jibril Sufyanu Muhammad Mustapha Jibril Aliyu Maje Bello Kabiru Abubakar Musa Ahmad Hussaini Jagaba Mohd Yunus Shukor Hafeez Muhammad Yakasai Bioremediation of lead contaminated environment by Bacillus cereus strain BUK_BCH_BTE2: Isolation and characterization of the bacterium Case Studies in Chemical and Environmental Engineering Lead Heavy metals Isolation Bacteria Lead-tolerant |
| title | Bioremediation of lead contaminated environment by Bacillus cereus strain BUK_BCH_BTE2: Isolation and characterization of the bacterium |
| title_full | Bioremediation of lead contaminated environment by Bacillus cereus strain BUK_BCH_BTE2: Isolation and characterization of the bacterium |
| title_fullStr | Bioremediation of lead contaminated environment by Bacillus cereus strain BUK_BCH_BTE2: Isolation and characterization of the bacterium |
| title_full_unstemmed | Bioremediation of lead contaminated environment by Bacillus cereus strain BUK_BCH_BTE2: Isolation and characterization of the bacterium |
| title_short | Bioremediation of lead contaminated environment by Bacillus cereus strain BUK_BCH_BTE2: Isolation and characterization of the bacterium |
| title_sort | bioremediation of lead contaminated environment by bacillus cereus strain buk bch bte2 isolation and characterization of the bacterium |
| topic | Lead Heavy metals Isolation Bacteria Lead-tolerant |
| url | http://www.sciencedirect.com/science/article/pii/S2666016423002451 |
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