First report on isolation of Mucor bainieri from honeybees, Apis mellifera: Characterization and biological activities
Fungi are potential biocontrol agents and rich sources of secondary metabolites with demonstrated biological activities. This study aimed to isolate and identify fungi from surface-sterilized honeybees (Apis mellifera), as well as to evaluate their biological activities. One fungal isolate was obtai...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Biotechnology Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215017X22000686 |
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| author | Mohamed H. Kalaba Mahmoud H. Sultan Mostafa A. Elbahnasawy Samia E. El-Didamony Nermeen M.El Bakary Mohamed H. Sharaf |
| author_facet | Mohamed H. Kalaba Mahmoud H. Sultan Mostafa A. Elbahnasawy Samia E. El-Didamony Nermeen M.El Bakary Mohamed H. Sharaf |
| author_sort | Mohamed H. Kalaba |
| collection | DOAJ |
| description | Fungi are potential biocontrol agents and rich sources of secondary metabolites with demonstrated biological activities. This study aimed to isolate and identify fungi from surface-sterilized honeybees (Apis mellifera), as well as to evaluate their biological activities. One fungal isolate was obtained and identified morphologically and genetically as Mucor bainieri MK-Bee-2. Gas chromatography-mass spectroscopy (GC–MS) analysis of fungus crude extract, showed the existence of six major metabolites representing 92.48% of the total peak area. The crude extract of Mucor bainieri MK-Bee-2 was tested for antimicrobial, antioxidant, and antitumor activities. It demonstrated wide antimicrobial activities against human pathogenic Gram-positive and Gram-negative bacterial strains, as well as Candida albicans, with MIC values ranged from 62.5 to 250 µg/ml. The results revealed that the extract exhibited considerable antioxidant activities indicated by strong inhibition of both DPPH and ABTS free radicals. Additionally, the extract exhibited greater potential anticancer activity against both adenocarcinomic human non-small cell lung cancer cells (A549) [IC50 = 6.45 μg/ml], and immortal cell line hepatoma G2 (HepG2) human liver cancer cells [IC50 = 27.48 μg/ml] and higher selectivity in cancer cells than normal cell lines. Furthermore, the extract showed less cytotoxic activity against normal cells with higher IC50 values of 106.99 and 132.57 μg/ml against human lung fibroblast Wistar-38 (Wi-38) and oral epithelial cells (OEC), respectively. Taken together, the Mucor bainieri MK-Bee-2 extract comprises bioactive compounds as promising potential therapeutic candidates for the treatment of lung cancer. Strikingly, the extract sensitizes the lung cancer cells A549 to the ionizing radiation through the pro-apoptotic pathway as indicated by the annexin V flow cytometry analysis which showed that the extract reduced the apoptosis of lung cancer cells. |
| first_indexed | 2024-04-11T23:51:05Z |
| format | Article |
| id | doaj.art-693c3ff96ea74cb295decc60f51c9a49 |
| institution | Directory Open Access Journal |
| issn | 2215-017X |
| language | English |
| last_indexed | 2024-04-11T23:51:05Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biotechnology Reports |
| spelling | doaj.art-693c3ff96ea74cb295decc60f51c9a492022-12-22T03:56:30ZengElsevierBiotechnology Reports2215-017X2022-12-0136e00770First report on isolation of Mucor bainieri from honeybees, Apis mellifera: Characterization and biological activitiesMohamed H. Kalaba0Mahmoud H. Sultan1Mostafa A. Elbahnasawy2Samia E. El-Didamony3Nermeen M.El Bakary4Mohamed H. Sharaf5Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Nasr City, Cairo 11884, Egypt; Corresponding authors.Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Nasr City, Cairo 11884, EgyptBotany and Microbiology Department, Faculty of Science, Al-Azhar University, Nasr City, Cairo 11884, Egypt; Corresponding authors.Zoology and Entomology Department, Faculty of Science, Al-Azhar University (Girls), Nasr City, Cairo 11884, EgyptRadiation Biology Department, National Center for Radiation Research and Technology, Egyptian Atomic Energy Authority, Cairo, Egypt; Corresponding authors.Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Nasr City, Cairo 11884, EgyptFungi are potential biocontrol agents and rich sources of secondary metabolites with demonstrated biological activities. This study aimed to isolate and identify fungi from surface-sterilized honeybees (Apis mellifera), as well as to evaluate their biological activities. One fungal isolate was obtained and identified morphologically and genetically as Mucor bainieri MK-Bee-2. Gas chromatography-mass spectroscopy (GC–MS) analysis of fungus crude extract, showed the existence of six major metabolites representing 92.48% of the total peak area. The crude extract of Mucor bainieri MK-Bee-2 was tested for antimicrobial, antioxidant, and antitumor activities. It demonstrated wide antimicrobial activities against human pathogenic Gram-positive and Gram-negative bacterial strains, as well as Candida albicans, with MIC values ranged from 62.5 to 250 µg/ml. The results revealed that the extract exhibited considerable antioxidant activities indicated by strong inhibition of both DPPH and ABTS free radicals. Additionally, the extract exhibited greater potential anticancer activity against both adenocarcinomic human non-small cell lung cancer cells (A549) [IC50 = 6.45 μg/ml], and immortal cell line hepatoma G2 (HepG2) human liver cancer cells [IC50 = 27.48 μg/ml] and higher selectivity in cancer cells than normal cell lines. Furthermore, the extract showed less cytotoxic activity against normal cells with higher IC50 values of 106.99 and 132.57 μg/ml against human lung fibroblast Wistar-38 (Wi-38) and oral epithelial cells (OEC), respectively. Taken together, the Mucor bainieri MK-Bee-2 extract comprises bioactive compounds as promising potential therapeutic candidates for the treatment of lung cancer. Strikingly, the extract sensitizes the lung cancer cells A549 to the ionizing radiation through the pro-apoptotic pathway as indicated by the annexin V flow cytometry analysis which showed that the extract reduced the apoptosis of lung cancer cells.http://www.sciencedirect.com/science/article/pii/S2215017X22000686FungiMucor bainieriHoneybeesApis melliferaAntimicrobial activityAntioxidant activity |
| spellingShingle | Mohamed H. Kalaba Mahmoud H. Sultan Mostafa A. Elbahnasawy Samia E. El-Didamony Nermeen M.El Bakary Mohamed H. Sharaf First report on isolation of Mucor bainieri from honeybees, Apis mellifera: Characterization and biological activities Biotechnology Reports Fungi Mucor bainieri Honeybees Apis mellifera Antimicrobial activity Antioxidant activity |
| title | First report on isolation of Mucor bainieri from honeybees, Apis mellifera: Characterization and biological activities |
| title_full | First report on isolation of Mucor bainieri from honeybees, Apis mellifera: Characterization and biological activities |
| title_fullStr | First report on isolation of Mucor bainieri from honeybees, Apis mellifera: Characterization and biological activities |
| title_full_unstemmed | First report on isolation of Mucor bainieri from honeybees, Apis mellifera: Characterization and biological activities |
| title_short | First report on isolation of Mucor bainieri from honeybees, Apis mellifera: Characterization and biological activities |
| title_sort | first report on isolation of mucor bainieri from honeybees apis mellifera characterization and biological activities |
| topic | Fungi Mucor bainieri Honeybees Apis mellifera Antimicrobial activity Antioxidant activity |
| url | http://www.sciencedirect.com/science/article/pii/S2215017X22000686 |
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