89Zr-panitumumab PET imaging for preoperative assessment of ameloblastoma in a PDX model
Abstract Accurate assessment of tumor margins with specific, non-invasive imaging would result in the preservation of healthy tissue and improve long-term local tumor control, thereby reducing the risk of recurrence. Overexpression of epidermal growth factor receptor (EGFR) has been used in other ca...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-11-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-23531-z |
| _version_ | 1798018392464031744 |
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| author | Logan D. Stone Adriana V. F. Massicano Todd M. Stevens Jason M. Warram Anthony B. Morlandt Suzanne E. Lapi Hope M. Amm |
| author_facet | Logan D. Stone Adriana V. F. Massicano Todd M. Stevens Jason M. Warram Anthony B. Morlandt Suzanne E. Lapi Hope M. Amm |
| author_sort | Logan D. Stone |
| collection | DOAJ |
| description | Abstract Accurate assessment of tumor margins with specific, non-invasive imaging would result in the preservation of healthy tissue and improve long-term local tumor control, thereby reducing the risk of recurrence. Overexpression of epidermal growth factor receptor (EGFR) has been used in other cancers as an imaging biomarker to identify cancerous tissue. We hypothesize that expression of EGFR in ameloblastomas may be used to specifically visualize tumors. The aims of this study are to measure the specificity of radiolabeled 89Zr-panitumumab (an EGFR antibody) in vivo using patient-derived xenograft (PDX) models of ameloblastoma and positron emission tomography/computed tomography (PET/CT) scans. In PDX of ameloblastomas from four patients (AB-36, AB-37, AB-39 AB-53), the biodistribution of 89Zr-panitumumab was measured 120 h post-injection and was reported as the injected dose per gram of tissue (%ID/g; AB-36, 40%; AB-37, 62%; AB-39 18%; AB-53, 65%). The radiolabeled %ID/g was significantly greater in tumors of 89Zr-panitumumab-treated mice that did not receive unlabeled panitumumab as a blocking control for AB-36, AB-37, and AB-53. Radiolabeled anti-EGFR demonstrates specificity for ameloblastoma PDX tumor xenografts, we believe 89Zr-panitumumab is an attractive target for pre-surgical imaging of ameloblastomas. With this technology, we could more accurately assess tumor margins for the surgical removal of ameloblastomas. |
| first_indexed | 2024-04-11T16:23:11Z |
| format | Article |
| id | doaj.art-693c5881257242cea986d58597852371 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-11T16:23:11Z |
| publishDate | 2022-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-693c5881257242cea986d585978523712022-12-22T04:14:16ZengNature PortfolioScientific Reports2045-23222022-11-011211910.1038/s41598-022-23531-z89Zr-panitumumab PET imaging for preoperative assessment of ameloblastoma in a PDX modelLogan D. Stone0Adriana V. F. Massicano1Todd M. Stevens2Jason M. Warram3Anthony B. Morlandt4Suzanne E. Lapi5Hope M. Amm6Department of Otolaryngology, University of Alabama at BirminghamDepartment of Radiology, University of Alabama at BirminghamDepartment of Pathology, University of Alabama at BirminghamDepartment of Otolaryngology, University of Alabama at BirminghamDepartment of Oral and Maxillofacial Surgery, Section of Oral Oncology, University of Alabama at BirminghamDepartment of Radiology, University of Alabama at BirminghamDepartment of Oral and Maxillofacial Surgery, Section of Oral Oncology, University of Alabama at BirminghamAbstract Accurate assessment of tumor margins with specific, non-invasive imaging would result in the preservation of healthy tissue and improve long-term local tumor control, thereby reducing the risk of recurrence. Overexpression of epidermal growth factor receptor (EGFR) has been used in other cancers as an imaging biomarker to identify cancerous tissue. We hypothesize that expression of EGFR in ameloblastomas may be used to specifically visualize tumors. The aims of this study are to measure the specificity of radiolabeled 89Zr-panitumumab (an EGFR antibody) in vivo using patient-derived xenograft (PDX) models of ameloblastoma and positron emission tomography/computed tomography (PET/CT) scans. In PDX of ameloblastomas from four patients (AB-36, AB-37, AB-39 AB-53), the biodistribution of 89Zr-panitumumab was measured 120 h post-injection and was reported as the injected dose per gram of tissue (%ID/g; AB-36, 40%; AB-37, 62%; AB-39 18%; AB-53, 65%). The radiolabeled %ID/g was significantly greater in tumors of 89Zr-panitumumab-treated mice that did not receive unlabeled panitumumab as a blocking control for AB-36, AB-37, and AB-53. Radiolabeled anti-EGFR demonstrates specificity for ameloblastoma PDX tumor xenografts, we believe 89Zr-panitumumab is an attractive target for pre-surgical imaging of ameloblastomas. With this technology, we could more accurately assess tumor margins for the surgical removal of ameloblastomas.https://doi.org/10.1038/s41598-022-23531-z |
| spellingShingle | Logan D. Stone Adriana V. F. Massicano Todd M. Stevens Jason M. Warram Anthony B. Morlandt Suzanne E. Lapi Hope M. Amm 89Zr-panitumumab PET imaging for preoperative assessment of ameloblastoma in a PDX model Scientific Reports |
| title | 89Zr-panitumumab PET imaging for preoperative assessment of ameloblastoma in a PDX model |
| title_full | 89Zr-panitumumab PET imaging for preoperative assessment of ameloblastoma in a PDX model |
| title_fullStr | 89Zr-panitumumab PET imaging for preoperative assessment of ameloblastoma in a PDX model |
| title_full_unstemmed | 89Zr-panitumumab PET imaging for preoperative assessment of ameloblastoma in a PDX model |
| title_short | 89Zr-panitumumab PET imaging for preoperative assessment of ameloblastoma in a PDX model |
| title_sort | 89zr panitumumab pet imaging for preoperative assessment of ameloblastoma in a pdx model |
| url | https://doi.org/10.1038/s41598-022-23531-z |
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