Identification of Mycobacterium tuberculosis isolated from culture-negative pulmonary and extra-pulmonary samples in cases of suspected tuberculosis
Introduction: is the cause of pulmonary and extra-pulmonary tuberculosis. Latent tuberculosis is asymptomatic and the results of mycobacterial culture in this case are negative. The purpose of this study was to determine the prevalence of in negative cultures of pulmonary and extra-pulmonary sampl...
| Main Authors: | , , |
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| Format: | Article |
| Language: | deu |
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German Medical Science GMS Publishing House
2019-08-01
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| Series: | GMS Hygiene and Infection Control |
| Subjects: | |
| Online Access: | http://www.egms.de/static/en/journals/dgkh/2019-14/dgkh000325.shtml |
| _version_ | 1818260535305568256 |
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| author | Harirzadeh, Saeed Kazemi, Mohammad Javad Babakhani, Sajad |
| author_facet | Harirzadeh, Saeed Kazemi, Mohammad Javad Babakhani, Sajad |
| author_sort | Harirzadeh, Saeed |
| collection | DOAJ |
| description | Introduction: is the cause of pulmonary and extra-pulmonary tuberculosis. Latent tuberculosis is asymptomatic and the results of mycobacterial culture in this case are negative. The purpose of this study was to determine the prevalence of in negative cultures of pulmonary and extra-pulmonary samples from suspected tuberculosis patients in Yazd city.Methods: Specimens were collected from 44 patients with suspected pulmonary and extra-pulmonary tuberculosis. The culture result of all samples was negative and DNA was extracted from culture-negative samples. Then the IS sequence of was determined by PCR, and the presence of 16S rRNA and A-PCL1 was checked by real-time PCR. Data were analyzed using SPSS software.Results: Of the 44 pulmonary and extra-pulmonary samples, DNA was detected in 12 cases (27%) by PCR. Of the 12 positive cases of , 9 were detected in lung samples and 3 in extra-pulmonary samples. For A-PCL1 and 16S rRNA genes, 10 samples (23%) were confirmed positive by real-time PCR. The frequency distributions of according to PCR and real-time PCR were not significantly different (p=0.403).Conclusion: Latent tuberculosis is characterized by the absence of clinical symptoms and the absence of bacilli in the culture. Regardless of the statistical analysis of results, PCR more effectively detects than does real-time PCR. According to the results, detection of in pulmonary and extra-pulmonary samples with culture-negative by PCR is reliable. |
| first_indexed | 2024-12-12T18:32:53Z |
| format | Article |
| id | doaj.art-693e3a5e693c488aab68e5258622bc24 |
| institution | Directory Open Access Journal |
| issn | 2196-5226 |
| language | deu |
| last_indexed | 2024-12-12T18:32:53Z |
| publishDate | 2019-08-01 |
| publisher | German Medical Science GMS Publishing House |
| record_format | Article |
| series | GMS Hygiene and Infection Control |
| spelling | doaj.art-693e3a5e693c488aab68e5258622bc242022-12-22T00:15:52ZdeuGerman Medical Science GMS Publishing HouseGMS Hygiene and Infection Control2196-52262019-08-0114Doc0910.3205/dgkh000325Identification of Mycobacterium tuberculosis isolated from culture-negative pulmonary and extra-pulmonary samples in cases of suspected tuberculosisHarirzadeh, Saeed0Kazemi, Mohammad Javad1Babakhani, Sajad2Medical Biotechnology Research Center, Ashkezar Branch, Islamic Azad University, Ashkezar, Yazd, IranMedical Biotechnology Research Center, Ashkezar Branch, Islamic Azad University, Ashkezar, Yazd, IranDepartment of Microbiology, North Tehran Branch, Islamic Azad University, Tehran, IranIntroduction: is the cause of pulmonary and extra-pulmonary tuberculosis. Latent tuberculosis is asymptomatic and the results of mycobacterial culture in this case are negative. The purpose of this study was to determine the prevalence of in negative cultures of pulmonary and extra-pulmonary samples from suspected tuberculosis patients in Yazd city.Methods: Specimens were collected from 44 patients with suspected pulmonary and extra-pulmonary tuberculosis. The culture result of all samples was negative and DNA was extracted from culture-negative samples. Then the IS sequence of was determined by PCR, and the presence of 16S rRNA and A-PCL1 was checked by real-time PCR. Data were analyzed using SPSS software.Results: Of the 44 pulmonary and extra-pulmonary samples, DNA was detected in 12 cases (27%) by PCR. Of the 12 positive cases of , 9 were detected in lung samples and 3 in extra-pulmonary samples. For A-PCL1 and 16S rRNA genes, 10 samples (23%) were confirmed positive by real-time PCR. The frequency distributions of according to PCR and real-time PCR were not significantly different (p=0.403).Conclusion: Latent tuberculosis is characterized by the absence of clinical symptoms and the absence of bacilli in the culture. Regardless of the statistical analysis of results, PCR more effectively detects than does real-time PCR. According to the results, detection of in pulmonary and extra-pulmonary samples with culture-negative by PCR is reliable.http://www.egms.de/static/en/journals/dgkh/2019-14/dgkh000325.shtmllatent tuberculosisMycobacterium tuberculosisPCRreal-time PCRrrnA-PCL1 |
| spellingShingle | Harirzadeh, Saeed Kazemi, Mohammad Javad Babakhani, Sajad Identification of Mycobacterium tuberculosis isolated from culture-negative pulmonary and extra-pulmonary samples in cases of suspected tuberculosis GMS Hygiene and Infection Control latent tuberculosis Mycobacterium tuberculosis PCR real-time PCR rrnA-PCL1 |
| title | Identification of Mycobacterium tuberculosis isolated from culture-negative pulmonary and extra-pulmonary samples in cases of suspected tuberculosis |
| title_full | Identification of Mycobacterium tuberculosis isolated from culture-negative pulmonary and extra-pulmonary samples in cases of suspected tuberculosis |
| title_fullStr | Identification of Mycobacterium tuberculosis isolated from culture-negative pulmonary and extra-pulmonary samples in cases of suspected tuberculosis |
| title_full_unstemmed | Identification of Mycobacterium tuberculosis isolated from culture-negative pulmonary and extra-pulmonary samples in cases of suspected tuberculosis |
| title_short | Identification of Mycobacterium tuberculosis isolated from culture-negative pulmonary and extra-pulmonary samples in cases of suspected tuberculosis |
| title_sort | identification of mycobacterium tuberculosis isolated from culture negative pulmonary and extra pulmonary samples in cases of suspected tuberculosis |
| topic | latent tuberculosis Mycobacterium tuberculosis PCR real-time PCR rrnA-PCL1 |
| url | http://www.egms.de/static/en/journals/dgkh/2019-14/dgkh000325.shtml |
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