Detection of Micrococcus Luteus Biofilm Formation in Microfluidic Environments by pH Measurement Using an Ion-Sensitive Field-Effect Transistor
Biofilm formation in microfluidic channels is difficult to detect because sampling volumes are too small for conventional turbidity measurements. To detect biofilm formation, we used an ion-sensitive field-effect transistor (ISFET) measurement system to measure pH changes in small volumes of bacteri...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2013-02-01
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| Series: | Sensors |
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| Online Access: | http://www.mdpi.com/1424-8220/13/2/2484 |
| _version_ | 1818034978357772288 |
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| author | Keiji Naruse Akira Yamada Yuka Asano Koji Matsuura |
| author_facet | Keiji Naruse Akira Yamada Yuka Asano Koji Matsuura |
| author_sort | Keiji Naruse |
| collection | DOAJ |
| description | Biofilm formation in microfluidic channels is difficult to detect because sampling volumes are too small for conventional turbidity measurements. To detect biofilm formation, we used an ion-sensitive field-effect transistor (ISFET) measurement system to measure pH changes in small volumes of bacterial suspension. Cells of Micrococcus luteus (M. luteus) were cultured in polystyrene (PS) microtubes and polymethylmethacrylate (PMMA)-based microfluidic channels laminated with polyvinylidene chloride. In microtubes, concentrations of bacteria and pH in the suspension were analyzed by measuring turbidity and using an ISFET sensor, respectively. In microfluidic channels containing 20 μL of bacterial suspension, we measured pH changes using the ISFET sensor and monitored biofilm formation using a microscope. We detected acidification and alkalinization phases of M. luteus from the ISFET sensor signals in both microtubes and microfluidic channels. In the alkalinization phase, after 2 day culture, dense biofilm formation was observed at the bottom of the microfluidic channels. In this study, we used an ISFET sensor to detect biofilm formation in clinical and industrial microfluidic environments by detecting alkalinization of the culture medium. |
| first_indexed | 2024-12-10T06:47:45Z |
| format | Article |
| id | doaj.art-6940d07212be43e688dcd64967dd92a6 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-12-10T06:47:45Z |
| publishDate | 2013-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-6940d07212be43e688dcd64967dd92a62022-12-22T01:58:37ZengMDPI AGSensors1424-82202013-02-011322484249310.3390/s130202484Detection of Micrococcus Luteus Biofilm Formation in Microfluidic Environments by pH Measurement Using an Ion-Sensitive Field-Effect TransistorKeiji NaruseAkira YamadaYuka AsanoKoji MatsuuraBiofilm formation in microfluidic channels is difficult to detect because sampling volumes are too small for conventional turbidity measurements. To detect biofilm formation, we used an ion-sensitive field-effect transistor (ISFET) measurement system to measure pH changes in small volumes of bacterial suspension. Cells of Micrococcus luteus (M. luteus) were cultured in polystyrene (PS) microtubes and polymethylmethacrylate (PMMA)-based microfluidic channels laminated with polyvinylidene chloride. In microtubes, concentrations of bacteria and pH in the suspension were analyzed by measuring turbidity and using an ISFET sensor, respectively. In microfluidic channels containing 20 μL of bacterial suspension, we measured pH changes using the ISFET sensor and monitored biofilm formation using a microscope. We detected acidification and alkalinization phases of M. luteus from the ISFET sensor signals in both microtubes and microfluidic channels. In the alkalinization phase, after 2 day culture, dense biofilm formation was observed at the bottom of the microfluidic channels. In this study, we used an ISFET sensor to detect biofilm formation in clinical and industrial microfluidic environments by detecting alkalinization of the culture medium.http://www.mdpi.com/1424-8220/13/2/2484ion-sensing field-effect transistorMicrococcus luteus biofilmalkalinizationmicrofluidic channel |
| spellingShingle | Keiji Naruse Akira Yamada Yuka Asano Koji Matsuura Detection of Micrococcus Luteus Biofilm Formation in Microfluidic Environments by pH Measurement Using an Ion-Sensitive Field-Effect Transistor Sensors ion-sensing field-effect transistor Micrococcus luteus biofilm alkalinization microfluidic channel |
| title | Detection of Micrococcus Luteus Biofilm Formation in Microfluidic Environments by pH Measurement Using an Ion-Sensitive Field-Effect Transistor |
| title_full | Detection of Micrococcus Luteus Biofilm Formation in Microfluidic Environments by pH Measurement Using an Ion-Sensitive Field-Effect Transistor |
| title_fullStr | Detection of Micrococcus Luteus Biofilm Formation in Microfluidic Environments by pH Measurement Using an Ion-Sensitive Field-Effect Transistor |
| title_full_unstemmed | Detection of Micrococcus Luteus Biofilm Formation in Microfluidic Environments by pH Measurement Using an Ion-Sensitive Field-Effect Transistor |
| title_short | Detection of Micrococcus Luteus Biofilm Formation in Microfluidic Environments by pH Measurement Using an Ion-Sensitive Field-Effect Transistor |
| title_sort | detection of micrococcus luteus biofilm formation in microfluidic environments by ph measurement using an ion sensitive field effect transistor |
| topic | ion-sensing field-effect transistor Micrococcus luteus biofilm alkalinization microfluidic channel |
| url | http://www.mdpi.com/1424-8220/13/2/2484 |
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