Characterizing the cellular and molecular variabilities of peripheral immune cells in healthy recipients of BBIBP-CorV inactivated SARS-CoV-2 vaccine by single-cell RNA sequencing
Over 3 billion doses of inactivated vaccines for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have been administered globally. However, our understanding of the immune cell functional transcription and T cell receptor (TCR)/B cell receptor (BCR) repertoire dynamics following inactiva...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Emerging Microbes and Infections |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2023.2187245 |
| _version_ | 1826898941959995392 |
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| author | Renyang Tong Lingjie Luo Yichao Zhao Mingze Sun Ronghong Li Jianmei Zhong Yifan Chen Liuhua Hu Zheng Li Jianfeng Shi Yuyan Lyu Li Hu Xiao Guo Qi Liu Tian Shuang Chenjie Zhang Ancai Yuan Lingyue Sun Zheng Zhang Kun Qian Lei Chen Wei Lin Alex F. Chen Feng Wang Jun Pu |
| author_facet | Renyang Tong Lingjie Luo Yichao Zhao Mingze Sun Ronghong Li Jianmei Zhong Yifan Chen Liuhua Hu Zheng Li Jianfeng Shi Yuyan Lyu Li Hu Xiao Guo Qi Liu Tian Shuang Chenjie Zhang Ancai Yuan Lingyue Sun Zheng Zhang Kun Qian Lei Chen Wei Lin Alex F. Chen Feng Wang Jun Pu |
| author_sort | Renyang Tong |
| collection | DOAJ |
| description | Over 3 billion doses of inactivated vaccines for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have been administered globally. However, our understanding of the immune cell functional transcription and T cell receptor (TCR)/B cell receptor (BCR) repertoire dynamics following inactivated SARS-CoV-2 vaccination remains poorly understood. Here, we performed single-cell RNA and TCR/BCR sequencing on peripheral blood mononuclear cells at four time points after immunization with the inactivated SARS-CoV-2 vaccine BBIBP-CorV. Our analysis revealed an enrichment of monocytes, central memory CD4+ T cells, type 2 helper T cells and memory B cells following vaccination. Single-cell TCR-seq and RNA-seq comminating analysis identified a clonal expansion of CD4+ T cells (but not CD8+ T cells) following a booster vaccination that corresponded to a decrease in the TCR diversity of central memory CD4+ T cells and type 2 helper T cells. Importantly, these TCR repertoire changes and CD4+ T cell differentiation were correlated with the biased VJ gene usage of BCR and the antibody-producing function of B cells post-vaccination. Finally, we compared the functional transcription and repertoire dynamics in immune cells elicited by vaccination and SARS-CoV-2 infection to explore the immune responses under different stimuli. Our data provide novel molecular and cellular evidence for the CD4+ T cell-dependent antibody response induced by inactivated vaccine BBIBP-CorV. This information is urgently needed to develop new prevention and control strategies for SARS-CoV-2 infection. (ClinicalTrials.gov Identifier: NCT04871932). |
| first_indexed | 2024-03-11T16:35:13Z |
| format | Article |
| id | doaj.art-6942ea9b203e4df1a7d0d43f272ba194 |
| institution | Directory Open Access Journal |
| issn | 2222-1751 |
| language | English |
| last_indexed | 2025-02-17T06:44:22Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj.art-6942ea9b203e4df1a7d0d43f272ba1942025-01-06T13:38:29ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512023-12-0112110.1080/22221751.2023.2187245Characterizing the cellular and molecular variabilities of peripheral immune cells in healthy recipients of BBIBP-CorV inactivated SARS-CoV-2 vaccine by single-cell RNA sequencingRenyang Tong0Lingjie Luo1Yichao Zhao2Mingze Sun3Ronghong Li4Jianmei Zhong5Yifan Chen6Liuhua Hu7Zheng Li8Jianfeng Shi9Yuyan Lyu10Li Hu11Xiao Guo12Qi Liu13Tian Shuang14Chenjie Zhang15Ancai Yuan16Lingyue Sun17Zheng Zhang18Kun Qian19Lei Chen20Wei Lin21Alex F. Chen22Feng Wang23Jun Pu24Division of Cardiology, Shanghai Immune Therapy Institute, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People’s Republic of ChinaShanghai Institute of Immunology, Department of Immunology and Microbiology, State Key Laboratory of Oncogenes and Related Genes, School of Medicine, Shanghai Jiao Tong University, Shanghai, People’s Republic of ChinaDivision of Cardiology, Shanghai Immune Therapy Institute, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People’s Republic of ChinaDivision of Cardiology, Shanghai Immune Therapy Institute, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People’s Republic of ChinaDivision of Cardiology, Shanghai Immune Therapy Institute, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People’s Republic of ChinaDivision of Cardiology, Shanghai Immune Therapy Institute, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People’s Republic of ChinaDivision of Cardiology, Shanghai Immune Therapy Institute, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People’s Republic of ChinaDivision of Cardiology, Shanghai Immune Therapy Institute, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People’s Republic of ChinaDivision of Cardiology, Shanghai Immune Therapy Institute, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People’s Republic of ChinaDivision of Cardiology, Shanghai Immune Therapy Institute, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People’s Republic of ChinaDivision of Cardiology, Shanghai Immune Therapy Institute, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People’s Republic of ChinaDivision of Cardiology, Shanghai Immune Therapy Institute, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People’s Republic of ChinaDivision of Cardiology, Shanghai Immune Therapy Institute, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People’s Republic of ChinaDivision of Cardiology, Shanghai Immune Therapy Institute, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People’s Republic of ChinaDivision of Cardiology, Shanghai Immune Therapy Institute, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People’s Republic of ChinaDivision of Cardiology, Shanghai Immune Therapy Institute, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People’s Republic of ChinaDivision of Cardiology, Shanghai Immune Therapy Institute, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People’s Republic of ChinaDivision of Cardiology, Shanghai Immune Therapy Institute, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People’s Republic of ChinaInstitute for Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People’s Hospital; The Second Affiliated Hospital, School of Medicine, Southern University of Science and Technology, Shenzhen, People’s Republic of ChinaDivision of Cardiology, Shanghai Immune Therapy Institute, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People’s Republic of ChinaDivision of Cardiology, Shanghai Immune Therapy Institute, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People’s Republic of ChinaDivision of Cardiology, Shanghai Immune Therapy Institute, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People’s Republic of ChinaInstitute for Developmental and Regenerative Cardiovascular Medicine, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People’s Republic of ChinaShanghai Institute of Immunology, Department of Immunology and Microbiology, State Key Laboratory of Oncogenes and Related Genes, School of Medicine, Shanghai Jiao Tong University, Shanghai, People’s Republic of ChinaDivision of Cardiology, Shanghai Immune Therapy Institute, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People’s Republic of ChinaOver 3 billion doses of inactivated vaccines for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have been administered globally. However, our understanding of the immune cell functional transcription and T cell receptor (TCR)/B cell receptor (BCR) repertoire dynamics following inactivated SARS-CoV-2 vaccination remains poorly understood. Here, we performed single-cell RNA and TCR/BCR sequencing on peripheral blood mononuclear cells at four time points after immunization with the inactivated SARS-CoV-2 vaccine BBIBP-CorV. Our analysis revealed an enrichment of monocytes, central memory CD4+ T cells, type 2 helper T cells and memory B cells following vaccination. Single-cell TCR-seq and RNA-seq comminating analysis identified a clonal expansion of CD4+ T cells (but not CD8+ T cells) following a booster vaccination that corresponded to a decrease in the TCR diversity of central memory CD4+ T cells and type 2 helper T cells. Importantly, these TCR repertoire changes and CD4+ T cell differentiation were correlated with the biased VJ gene usage of BCR and the antibody-producing function of B cells post-vaccination. Finally, we compared the functional transcription and repertoire dynamics in immune cells elicited by vaccination and SARS-CoV-2 infection to explore the immune responses under different stimuli. Our data provide novel molecular and cellular evidence for the CD4+ T cell-dependent antibody response induced by inactivated vaccine BBIBP-CorV. This information is urgently needed to develop new prevention and control strategies for SARS-CoV-2 infection. (ClinicalTrials.gov Identifier: NCT04871932).https://www.tandfonline.com/doi/10.1080/22221751.2023.2187245Inactivated SARS-CoV-2 vaccineBBIBP-CorVsingle-cell RNA sequencingsingle-cell TCR/BCR sequencingperipheral blood mononuclear cells |
| spellingShingle | Renyang Tong Lingjie Luo Yichao Zhao Mingze Sun Ronghong Li Jianmei Zhong Yifan Chen Liuhua Hu Zheng Li Jianfeng Shi Yuyan Lyu Li Hu Xiao Guo Qi Liu Tian Shuang Chenjie Zhang Ancai Yuan Lingyue Sun Zheng Zhang Kun Qian Lei Chen Wei Lin Alex F. Chen Feng Wang Jun Pu Characterizing the cellular and molecular variabilities of peripheral immune cells in healthy recipients of BBIBP-CorV inactivated SARS-CoV-2 vaccine by single-cell RNA sequencing Emerging Microbes and Infections Inactivated SARS-CoV-2 vaccine BBIBP-CorV single-cell RNA sequencing single-cell TCR/BCR sequencing peripheral blood mononuclear cells |
| title | Characterizing the cellular and molecular variabilities of peripheral immune cells in healthy recipients of BBIBP-CorV inactivated SARS-CoV-2 vaccine by single-cell RNA sequencing |
| title_full | Characterizing the cellular and molecular variabilities of peripheral immune cells in healthy recipients of BBIBP-CorV inactivated SARS-CoV-2 vaccine by single-cell RNA sequencing |
| title_fullStr | Characterizing the cellular and molecular variabilities of peripheral immune cells in healthy recipients of BBIBP-CorV inactivated SARS-CoV-2 vaccine by single-cell RNA sequencing |
| title_full_unstemmed | Characterizing the cellular and molecular variabilities of peripheral immune cells in healthy recipients of BBIBP-CorV inactivated SARS-CoV-2 vaccine by single-cell RNA sequencing |
| title_short | Characterizing the cellular and molecular variabilities of peripheral immune cells in healthy recipients of BBIBP-CorV inactivated SARS-CoV-2 vaccine by single-cell RNA sequencing |
| title_sort | characterizing the cellular and molecular variabilities of peripheral immune cells in healthy recipients of bbibp corv inactivated sars cov 2 vaccine by single cell rna sequencing |
| topic | Inactivated SARS-CoV-2 vaccine BBIBP-CorV single-cell RNA sequencing single-cell TCR/BCR sequencing peripheral blood mononuclear cells |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2023.2187245 |
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