Regeneration of periodontal ligament fibers around mini dental implants and their attachment to the bone in an animal model: A radiographic and histological study
Background: Tissue-engineered periodontal ligament (PDL) around a dental implant by using PDL stem cells (PDLSCs) may be useful in periodontal regeneration and can reduce or eliminate certain shortcomings of dental implants. Materials and Methods: PDLSCs were isolated from extracted human PDL cells...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2023-01-01
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| Series: | Journal of Indian Society of Periodontology |
| Subjects: | |
| Online Access: | http://www.jisponline.com/article.asp?issn=0972-124X;year=2023;volume=27;issue=2;spage=167;epage=173;aulast=Kukreja |
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| author | Bhavna Jha Kukreja Kishore G Bhat Pankaj Kukreja Ajaykumar Nayak Vijaylakshmi S Kotrashetty Santosh Dindawar Rajkumar Balakrishnan |
| author_facet | Bhavna Jha Kukreja Kishore G Bhat Pankaj Kukreja Ajaykumar Nayak Vijaylakshmi S Kotrashetty Santosh Dindawar Rajkumar Balakrishnan |
| author_sort | Bhavna Jha Kukreja |
| collection | DOAJ |
| description | Background: Tissue-engineered periodontal ligament (PDL) around a dental implant by using PDL stem cells (PDLSCs) may be useful in periodontal regeneration and can reduce or eliminate certain shortcomings of dental implants. Materials and Methods: PDLSCs were isolated from extracted human PDL cells and cultured in a bioreactor. They were identified using markers CD45, CD73, CD90, CD105, and CD146. After the formation of multiple cellular layers, they were then attached on titanium mini dental implants and placed in rabbit tibia. The rabbits were sacrificed after 9 months, and the implants were analyzed histologically and radiographically by Cone beam computed tomography (CBCT). Results: Isolated PDLSCs obtained from human premolars showed a colony-forming ability on the 7th day and 14th day. Immunocytochemistry revealed that cells had taken up the adequate positive stains for primary antibodies CD73, CD90, CD105, and CD146 and negative staining for CD45. The histological sections obtained from sacrificed rabbits, when viewed under the light microscope, clearly showed the presence of PDL around dental implants. CBCT examination showed that the implant was well within the bone and did not migrate. The site appeared to be normal without any lytic changes in the bone. Conclusion: It can safely be postulated from the present study that tissue engineering of PDL can be achieved around dental implants using PDLSCs. Important inter-tissue interactions like the formation of a functional PDL around the implantation site, and induction of bone formation in the vicinity of the implants may be possible. Future research in humans is required for further research. |
| first_indexed | 2024-03-12T22:36:27Z |
| format | Article |
| id | doaj.art-6943dd6f291149d6ab055c629740994a |
| institution | Directory Open Access Journal |
| issn | 0972-124X |
| language | English |
| last_indexed | 2024-03-12T22:36:27Z |
| publishDate | 2023-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Indian Society of Periodontology |
| spelling | doaj.art-6943dd6f291149d6ab055c629740994a2023-07-21T11:46:01ZengWolters Kluwer Medknow PublicationsJournal of Indian Society of Periodontology0972-124X2023-01-0127216717310.4103/jisp.jisp_314_22Regeneration of periodontal ligament fibers around mini dental implants and their attachment to the bone in an animal model: A radiographic and histological studyBhavna Jha KukrejaKishore G BhatPankaj KukrejaAjaykumar NayakVijaylakshmi S KotrashettySantosh DindawarRajkumar BalakrishnanBackground: Tissue-engineered periodontal ligament (PDL) around a dental implant by using PDL stem cells (PDLSCs) may be useful in periodontal regeneration and can reduce or eliminate certain shortcomings of dental implants. Materials and Methods: PDLSCs were isolated from extracted human PDL cells and cultured in a bioreactor. They were identified using markers CD45, CD73, CD90, CD105, and CD146. After the formation of multiple cellular layers, they were then attached on titanium mini dental implants and placed in rabbit tibia. The rabbits were sacrificed after 9 months, and the implants were analyzed histologically and radiographically by Cone beam computed tomography (CBCT). Results: Isolated PDLSCs obtained from human premolars showed a colony-forming ability on the 7th day and 14th day. Immunocytochemistry revealed that cells had taken up the adequate positive stains for primary antibodies CD73, CD90, CD105, and CD146 and negative staining for CD45. The histological sections obtained from sacrificed rabbits, when viewed under the light microscope, clearly showed the presence of PDL around dental implants. CBCT examination showed that the implant was well within the bone and did not migrate. The site appeared to be normal without any lytic changes in the bone. Conclusion: It can safely be postulated from the present study that tissue engineering of PDL can be achieved around dental implants using PDLSCs. Important inter-tissue interactions like the formation of a functional PDL around the implantation site, and induction of bone formation in the vicinity of the implants may be possible. Future research in humans is required for further research.http://www.jisponline.com/article.asp?issn=0972-124X;year=2023;volume=27;issue=2;spage=167;epage=173;aulast=Kukrejaimplantologyperiodontal ligamentstem cell |
| spellingShingle | Bhavna Jha Kukreja Kishore G Bhat Pankaj Kukreja Ajaykumar Nayak Vijaylakshmi S Kotrashetty Santosh Dindawar Rajkumar Balakrishnan Regeneration of periodontal ligament fibers around mini dental implants and their attachment to the bone in an animal model: A radiographic and histological study Journal of Indian Society of Periodontology implantology periodontal ligament stem cell |
| title | Regeneration of periodontal ligament fibers around mini dental implants and their attachment to the bone in an animal model: A radiographic and histological study |
| title_full | Regeneration of periodontal ligament fibers around mini dental implants and their attachment to the bone in an animal model: A radiographic and histological study |
| title_fullStr | Regeneration of periodontal ligament fibers around mini dental implants and their attachment to the bone in an animal model: A radiographic and histological study |
| title_full_unstemmed | Regeneration of periodontal ligament fibers around mini dental implants and their attachment to the bone in an animal model: A radiographic and histological study |
| title_short | Regeneration of periodontal ligament fibers around mini dental implants and their attachment to the bone in an animal model: A radiographic and histological study |
| title_sort | regeneration of periodontal ligament fibers around mini dental implants and their attachment to the bone in an animal model a radiographic and histological study |
| topic | implantology periodontal ligament stem cell |
| url | http://www.jisponline.com/article.asp?issn=0972-124X;year=2023;volume=27;issue=2;spage=167;epage=173;aulast=Kukreja |
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