Efficacy of combined HBsAg, anti-HBc and anti-HBs screening in minimizing transfusion transmission risk of hepatitis B infection in low resource setting
Background: Hepatitis B Virus (HBV), and occult Hepatitis B in particular, is a major concern in the transfusion scenario, especially in endemic countries. This study attempted to estimate the prevalence of occult Hepatitis B infection (OBI) among voluntary blood donors in Maharashtra and to evaluat...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-02-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S240584402401836X |
| _version_ | 1827348289838645248 |
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| author | Shreyasi Athalye Amruta Patil Naveen Khargekar Shailesh Shinde Shreya Chavan Abhay Dixit Aruna Shankarkumar Manisha Madkaikar Anindita Banerjee |
| author_facet | Shreyasi Athalye Amruta Patil Naveen Khargekar Shailesh Shinde Shreya Chavan Abhay Dixit Aruna Shankarkumar Manisha Madkaikar Anindita Banerjee |
| author_sort | Shreyasi Athalye |
| collection | DOAJ |
| description | Background: Hepatitis B Virus (HBV), and occult Hepatitis B in particular, is a major concern in the transfusion scenario, especially in endemic countries. This study attempted to estimate the prevalence of occult Hepatitis B infection (OBI) among voluntary blood donors in Maharashtra and to evaluate the role of combined screening strategy with implications in minimizing the current transfusion risks of seropositive OBI. Methods: Donor samples were collected from 80 eligible blood banks from various districts of Maharashtra between 2014 and 2017. ELISA based screening of HBsAg, anti-HBc (total and IgM), anti-HBs titres. Real-time quantitative PCR for Hepatitis B Virus DNA (HBV DNA) were performed for all HBsAg and or anti-HBc positive samples. Results: Out of 2398 samples tested, 20 (0.83%) samples were positive for HBsAg, whereas 547 (22.81%) were positive for anti-HBc. Out of 547 samples, 16 (2.92%) were positive for HBV DNA with median level at 247.89 IU/mL (IQR: 126.05–666.67 IU/mL). Anti-HBs levels were positive in 35.83% of OBI cases. ROC curve analysis showed that combined HBsAg, anti-HBc and anti-HBs (>50 mIU/mL) screening can more efficiently detect HBV infection in blood donors than HBsAg alone. Conclusions: A combined HBsAg, anti-HBc and anti-HBs screening for donor samples could be an alternative achievable strategy to minimize the HBV transmission as well as financial burden. In resource limited setup, the proposed combined strategy could be helpful in minimizing the risk of OBI transmission. |
| first_indexed | 2024-03-08T00:09:10Z |
| format | Article |
| id | doaj.art-6949d26214664ec994a01c6226f31091 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-08T00:09:10Z |
| publishDate | 2024-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-6949d26214664ec994a01c6226f310912024-02-17T06:41:42ZengElsevierHeliyon2405-84402024-02-01103e25805Efficacy of combined HBsAg, anti-HBc and anti-HBs screening in minimizing transfusion transmission risk of hepatitis B infection in low resource settingShreyasi Athalye0Amruta Patil1Naveen Khargekar2Shailesh Shinde3Shreya Chavan4Abhay Dixit5Aruna Shankarkumar6Manisha Madkaikar7Anindita Banerjee8Transfusion Transmission Disease Department, ICMR- National Institute of Immunohaematology, Mumbai, Maharashtra, 400 012, IndiaDepartment of Medical Biotechnology, MGM Institute of Health Sciences, Navi Mumbai, Maharashtra, IndiaHematogenetics Department, ICMR-National Institute of Immunohaematology, Mumbai, Maharashtra, 400 012, IndiaTransfusion Transmission Disease Department, ICMR- National Institute of Immunohaematology, Mumbai, Maharashtra, 400 012, IndiaTransfusion Transmission Disease Department, ICMR- National Institute of Immunohaematology, Mumbai, Maharashtra, 400 012, IndiaProject ACCELERATE, YRGCARE, Mumbai, Maharashtra, IndiaTransfusion Transmission Disease Department, ICMR- National Institute of Immunohaematology, Mumbai, Maharashtra, 400 012, IndiaPaediatric Immunology and Leukocyte Biology, ICMR- National Institute of Immunohaematology, Mumbai, Maharashtra, 400 012, IndiaTransfusion Transmission Disease Department, ICMR- National Institute of Immunohaematology, Mumbai, Maharashtra, 400 012, India; Corresponding author. Transfusion Transmission Disease Department, ICMR- National Institute of Immunohaematology, 13th Floor, New MS Building, KEM Hospital Campus, Parel, Mumbai, 400 012, India.Background: Hepatitis B Virus (HBV), and occult Hepatitis B in particular, is a major concern in the transfusion scenario, especially in endemic countries. This study attempted to estimate the prevalence of occult Hepatitis B infection (OBI) among voluntary blood donors in Maharashtra and to evaluate the role of combined screening strategy with implications in minimizing the current transfusion risks of seropositive OBI. Methods: Donor samples were collected from 80 eligible blood banks from various districts of Maharashtra between 2014 and 2017. ELISA based screening of HBsAg, anti-HBc (total and IgM), anti-HBs titres. Real-time quantitative PCR for Hepatitis B Virus DNA (HBV DNA) were performed for all HBsAg and or anti-HBc positive samples. Results: Out of 2398 samples tested, 20 (0.83%) samples were positive for HBsAg, whereas 547 (22.81%) were positive for anti-HBc. Out of 547 samples, 16 (2.92%) were positive for HBV DNA with median level at 247.89 IU/mL (IQR: 126.05–666.67 IU/mL). Anti-HBs levels were positive in 35.83% of OBI cases. ROC curve analysis showed that combined HBsAg, anti-HBc and anti-HBs (>50 mIU/mL) screening can more efficiently detect HBV infection in blood donors than HBsAg alone. Conclusions: A combined HBsAg, anti-HBc and anti-HBs screening for donor samples could be an alternative achievable strategy to minimize the HBV transmission as well as financial burden. In resource limited setup, the proposed combined strategy could be helpful in minimizing the risk of OBI transmission.http://www.sciencedirect.com/science/article/pii/S240584402401836XOccult hepatitis BHepatitis B virusBlood safetyanti-HBc screeningOBI transmission |
| spellingShingle | Shreyasi Athalye Amruta Patil Naveen Khargekar Shailesh Shinde Shreya Chavan Abhay Dixit Aruna Shankarkumar Manisha Madkaikar Anindita Banerjee Efficacy of combined HBsAg, anti-HBc and anti-HBs screening in minimizing transfusion transmission risk of hepatitis B infection in low resource setting Heliyon Occult hepatitis B Hepatitis B virus Blood safety anti-HBc screening OBI transmission |
| title | Efficacy of combined HBsAg, anti-HBc and anti-HBs screening in minimizing transfusion transmission risk of hepatitis B infection in low resource setting |
| title_full | Efficacy of combined HBsAg, anti-HBc and anti-HBs screening in minimizing transfusion transmission risk of hepatitis B infection in low resource setting |
| title_fullStr | Efficacy of combined HBsAg, anti-HBc and anti-HBs screening in minimizing transfusion transmission risk of hepatitis B infection in low resource setting |
| title_full_unstemmed | Efficacy of combined HBsAg, anti-HBc and anti-HBs screening in minimizing transfusion transmission risk of hepatitis B infection in low resource setting |
| title_short | Efficacy of combined HBsAg, anti-HBc and anti-HBs screening in minimizing transfusion transmission risk of hepatitis B infection in low resource setting |
| title_sort | efficacy of combined hbsag anti hbc and anti hbs screening in minimizing transfusion transmission risk of hepatitis b infection in low resource setting |
| topic | Occult hepatitis B Hepatitis B virus Blood safety anti-HBc screening OBI transmission |
| url | http://www.sciencedirect.com/science/article/pii/S240584402401836X |
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