OsZIP1 functions as a metal efflux transporter limiting excess zinc, copper and cadmium accumulation in rice
Abstract Background Metal homeostasis is critical for plant growth, development and adaptation to environmental stresses and largely governed by a variety of metal transporters. The plant ZIP (Zn-regulated transporter, Iron-regulated transporter-like Protein) family proteins belong to the integral m...
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| Format: | Article |
| Language: | English |
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BMC
2019-06-01
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| Series: | BMC Plant Biology |
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| Online Access: | http://link.springer.com/article/10.1186/s12870-019-1899-3 |
| _version_ | 1818244409834078208 |
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| author | Xue Song Liu Sheng Jun Feng Bai Qing Zhang Meng Qi Wang Hong Wei Cao Justice Kipkoir Rono Xi Chen Zhi Min Yang |
| author_facet | Xue Song Liu Sheng Jun Feng Bai Qing Zhang Meng Qi Wang Hong Wei Cao Justice Kipkoir Rono Xi Chen Zhi Min Yang |
| author_sort | Xue Song Liu |
| collection | DOAJ |
| description | Abstract Background Metal homeostasis is critical for plant growth, development and adaptation to environmental stresses and largely governed by a variety of metal transporters. The plant ZIP (Zn-regulated transporter, Iron-regulated transporter-like Protein) family proteins belong to the integral membrane transporters responsible for uptake and allocation of essential and non-essential metals. However, whether the ZIP family members mediate metal efflux and its regulatory mechanism remains unknown. Results In this report, we provided evidence that OsZIP1 is a metal-detoxified transporter through preventing excess Zn, Cu and Cd accumulation in rice. OsZIP1 is abundantly expressed in roots throughout the life span and sufficiently induced by excess Zn, Cu and Cd but not by Mn and Fe at transcriptional and translational levels. Expression of OsZIP-GFP fusion in rice protoplasts and tobacco leaves shows that OsZIP1 resides in the endoplasmic reticulum (ER) and plasma membrane (PM). The yeast (Saccharomyces cerevisiae) complementation test shows that expression of OsZIP1 reduced Zn accumulation. Transgenic rice overexpressing OsZIP1 grew better under excess metal stress but accumulated less of the metals in plants. In contrast, both oszip1 mutant and RNA interference (RNAi) lines accumulated more metal in roots and contributed to metal sensitive phenotypes. These results suggest OsZIP1 is able to function as a metal exporter in rice when Zn, Cu and Cd are excess in environment. We further identified the DNA methylation of histone H3K9me2 of OsZIP1 and found that OsZIP1 locus, whose transcribed regions imbed a 242 bp sequence, is demethylated, suggesting that epigenetic modification is likely associated with OsZIP1 function under Cd stress. Conclusion OsZIP1 is a transporter that is required for detoxification of excess Zn, Cu and Cd in rice. |
| first_indexed | 2024-12-12T14:16:34Z |
| format | Article |
| id | doaj.art-694ab6353c044e73b7d84f26e041a33d |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-12T14:16:34Z |
| publishDate | 2019-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-694ab6353c044e73b7d84f26e041a33d2022-12-22T00:21:53ZengBMCBMC Plant Biology1471-22292019-06-0119111610.1186/s12870-019-1899-3OsZIP1 functions as a metal efflux transporter limiting excess zinc, copper and cadmium accumulation in riceXue Song Liu0Sheng Jun Feng1Bai Qing Zhang2Meng Qi Wang3Hong Wei Cao4Justice Kipkoir Rono5Xi Chen6Zhi Min Yang7Department of Biochemistry and Molecular Biology, College of Life Science, Nanjing Agricultural UniversityDepartment of Biochemistry and Molecular Biology, College of Life Science, Nanjing Agricultural UniversityDepartment of Biochemistry and Molecular Biology, College of Life Science, Nanjing Agricultural UniversityDepartment of Biochemistry and Molecular Biology, College of Life Science, Nanjing Agricultural UniversityDepartment of Biochemistry and Molecular Biology, College of Life Science, Nanjing Agricultural UniversityDepartment of Biochemistry and Molecular Biology, College of Life Science, Nanjing Agricultural UniversityDepartment of Biochemistry and Molecular Biology, College of Life Science, Nanjing Agricultural UniversityDepartment of Biochemistry and Molecular Biology, College of Life Science, Nanjing Agricultural UniversityAbstract Background Metal homeostasis is critical for plant growth, development and adaptation to environmental stresses and largely governed by a variety of metal transporters. The plant ZIP (Zn-regulated transporter, Iron-regulated transporter-like Protein) family proteins belong to the integral membrane transporters responsible for uptake and allocation of essential and non-essential metals. However, whether the ZIP family members mediate metal efflux and its regulatory mechanism remains unknown. Results In this report, we provided evidence that OsZIP1 is a metal-detoxified transporter through preventing excess Zn, Cu and Cd accumulation in rice. OsZIP1 is abundantly expressed in roots throughout the life span and sufficiently induced by excess Zn, Cu and Cd but not by Mn and Fe at transcriptional and translational levels. Expression of OsZIP-GFP fusion in rice protoplasts and tobacco leaves shows that OsZIP1 resides in the endoplasmic reticulum (ER) and plasma membrane (PM). The yeast (Saccharomyces cerevisiae) complementation test shows that expression of OsZIP1 reduced Zn accumulation. Transgenic rice overexpressing OsZIP1 grew better under excess metal stress but accumulated less of the metals in plants. In contrast, both oszip1 mutant and RNA interference (RNAi) lines accumulated more metal in roots and contributed to metal sensitive phenotypes. These results suggest OsZIP1 is able to function as a metal exporter in rice when Zn, Cu and Cd are excess in environment. We further identified the DNA methylation of histone H3K9me2 of OsZIP1 and found that OsZIP1 locus, whose transcribed regions imbed a 242 bp sequence, is demethylated, suggesting that epigenetic modification is likely associated with OsZIP1 function under Cd stress. Conclusion OsZIP1 is a transporter that is required for detoxification of excess Zn, Cu and Cd in rice.http://link.springer.com/article/10.1186/s12870-019-1899-3OsZIP1RiceEndoplasmic reticulumMetal effluxCadmiumDNA demethylation |
| spellingShingle | Xue Song Liu Sheng Jun Feng Bai Qing Zhang Meng Qi Wang Hong Wei Cao Justice Kipkoir Rono Xi Chen Zhi Min Yang OsZIP1 functions as a metal efflux transporter limiting excess zinc, copper and cadmium accumulation in rice BMC Plant Biology OsZIP1 Rice Endoplasmic reticulum Metal efflux Cadmium DNA demethylation |
| title | OsZIP1 functions as a metal efflux transporter limiting excess zinc, copper and cadmium accumulation in rice |
| title_full | OsZIP1 functions as a metal efflux transporter limiting excess zinc, copper and cadmium accumulation in rice |
| title_fullStr | OsZIP1 functions as a metal efflux transporter limiting excess zinc, copper and cadmium accumulation in rice |
| title_full_unstemmed | OsZIP1 functions as a metal efflux transporter limiting excess zinc, copper and cadmium accumulation in rice |
| title_short | OsZIP1 functions as a metal efflux transporter limiting excess zinc, copper and cadmium accumulation in rice |
| title_sort | oszip1 functions as a metal efflux transporter limiting excess zinc copper and cadmium accumulation in rice |
| topic | OsZIP1 Rice Endoplasmic reticulum Metal efflux Cadmium DNA demethylation |
| url | http://link.springer.com/article/10.1186/s12870-019-1899-3 |
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