In vitro anti-reovirus activity of kuraridin isolated from Sophora flavescens against viral replication and hemagglutination
In this study, we evaluated the anti-reovirus activity of kuraridin isolated from the roots of Sophora flavescens. In particular, we focused on whether this property is attributable to direct inhibition of reovirus attachment and/or inhibition of viral replication with the aid of time-of-addition (p...
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| Format: | Article |
| Language: | English |
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Elsevier
2015-08-01
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| Series: | Journal of Pharmacological Sciences |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1347861315000869 |
| _version_ | 1818153383515652096 |
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| author | Hyung-Jun Kwon Jae-Ho Jeong Seung Woong Lee Young Bae Ryu Hyung Jae Jeong Kyungsook Jung Jae Sung Lim Kyoung-Oh Cho Woo Song Lee Mun-Chual Rho Su-Jin Park |
| author_facet | Hyung-Jun Kwon Jae-Ho Jeong Seung Woong Lee Young Bae Ryu Hyung Jae Jeong Kyungsook Jung Jae Sung Lim Kyoung-Oh Cho Woo Song Lee Mun-Chual Rho Su-Jin Park |
| author_sort | Hyung-Jun Kwon |
| collection | DOAJ |
| description | In this study, we evaluated the anti-reovirus activity of kuraridin isolated from the roots of Sophora flavescens. In particular, we focused on whether this property is attributable to direct inhibition of reovirus attachment and/or inhibition of viral replication with the aid of time-of-addition (pre-treatment, simultaneous treatment, and post-treatment) experiments. No significant antiviral activity of kuraridin was detected in the pre-treatment assay. In the simultaneous assay, the 50% effective inhibitory concentrations (EC50) of kuraridin were 15.3–176.9 μM against human type 1–3 reoviruses (HRV1–3) and Korean porcine reovirus (PRV). Kuraridin completely blocked binding of viral sigma 1 protein to sialic acids at concentrations lower than 82.5 μM in the hemagglutination inhibition assay. Moreover, kuraridin inhibited HRV1–3 and PRV viral replication with EC50 values of 14.0–62.0 μM. Quantitative real-time PCR analysis disclosed strong suppression of reovirus RNA synthesis at the late stage (18 h) of virus replication by kuraridin. The viral yields of kuraridin-treated cells were significantly reduced at 24 h post-infection, compared with DMSO-treated cells. Our results collectively suggest that kuraridin inhibits virus adsorption and replication by inhibiting hemagglutination, viral RNA and protein synthesis and virus shedding, supporting its utility as a viable candidate antiviral drug against reoviruses. |
| first_indexed | 2024-12-11T14:09:45Z |
| format | Article |
| id | doaj.art-694b52870bd14105b98c1302b24ce79e |
| institution | Directory Open Access Journal |
| issn | 1347-8613 |
| language | English |
| last_indexed | 2024-12-11T14:09:45Z |
| publishDate | 2015-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Pharmacological Sciences |
| spelling | doaj.art-694b52870bd14105b98c1302b24ce79e2022-12-22T01:03:30ZengElsevierJournal of Pharmacological Sciences1347-86132015-08-01128415916910.1016/j.jphs.2015.04.007In vitro anti-reovirus activity of kuraridin isolated from Sophora flavescens against viral replication and hemagglutinationHyung-Jun Kwon0Jae-Ho Jeong1Seung Woong Lee2Young Bae Ryu3Hyung Jae Jeong4Kyungsook Jung5Jae Sung Lim6Kyoung-Oh Cho7Woo Song Lee8Mun-Chual Rho9Su-Jin Park10Eco-friendly Bio-material Research Center, Korea Research Institute of Bioscience and Biotechnology, 181 Ipsin-gil, Jeongeup-si, Jeonbuk 580-185, Republic of KoreaDepartment of Microbiology, Chonnam National University Medical School, Gwangju 501-746, Republic of KoreaDepartment of Chemistry, Mokwon University, Daejeon 302-729, Republic of KoreaEco-friendly Bio-material Research Center, Korea Research Institute of Bioscience and Biotechnology, 181 Ipsin-gil, Jeongeup-si, Jeonbuk 580-185, Republic of KoreaEco-friendly Bio-material Research Center, Korea Research Institute of Bioscience and Biotechnology, 181 Ipsin-gil, Jeongeup-si, Jeonbuk 580-185, Republic of KoreaEco-friendly Bio-material Research Center, Korea Research Institute of Bioscience and Biotechnology, 181 Ipsin-gil, Jeongeup-si, Jeonbuk 580-185, Republic of KoreaDepartment of Biochemistry, Chonnam National University Medical School, Gwangju, Republic of KoreaBiotherapy Human Resources Center, College of Veterinary Medicine, Chonnam National University, Gwangju 500-757, Republic of KoreaEco-friendly Bio-material Research Center, Korea Research Institute of Bioscience and Biotechnology, 181 Ipsin-gil, Jeongeup-si, Jeonbuk 580-185, Republic of KoreaEco-friendly Bio-material Research Center, Korea Research Institute of Bioscience and Biotechnology, 181 Ipsin-gil, Jeongeup-si, Jeonbuk 580-185, Republic of KoreaEco-friendly Bio-material Research Center, Korea Research Institute of Bioscience and Biotechnology, 181 Ipsin-gil, Jeongeup-si, Jeonbuk 580-185, Republic of KoreaIn this study, we evaluated the anti-reovirus activity of kuraridin isolated from the roots of Sophora flavescens. In particular, we focused on whether this property is attributable to direct inhibition of reovirus attachment and/or inhibition of viral replication with the aid of time-of-addition (pre-treatment, simultaneous treatment, and post-treatment) experiments. No significant antiviral activity of kuraridin was detected in the pre-treatment assay. In the simultaneous assay, the 50% effective inhibitory concentrations (EC50) of kuraridin were 15.3–176.9 μM against human type 1–3 reoviruses (HRV1–3) and Korean porcine reovirus (PRV). Kuraridin completely blocked binding of viral sigma 1 protein to sialic acids at concentrations lower than 82.5 μM in the hemagglutination inhibition assay. Moreover, kuraridin inhibited HRV1–3 and PRV viral replication with EC50 values of 14.0–62.0 μM. Quantitative real-time PCR analysis disclosed strong suppression of reovirus RNA synthesis at the late stage (18 h) of virus replication by kuraridin. The viral yields of kuraridin-treated cells were significantly reduced at 24 h post-infection, compared with DMSO-treated cells. Our results collectively suggest that kuraridin inhibits virus adsorption and replication by inhibiting hemagglutination, viral RNA and protein synthesis and virus shedding, supporting its utility as a viable candidate antiviral drug against reoviruses.http://www.sciencedirect.com/science/article/pii/S1347861315000869Anti-reovirus activitySophora flavescensKuraridinViral absorption and replicationHemagglutination |
| spellingShingle | Hyung-Jun Kwon Jae-Ho Jeong Seung Woong Lee Young Bae Ryu Hyung Jae Jeong Kyungsook Jung Jae Sung Lim Kyoung-Oh Cho Woo Song Lee Mun-Chual Rho Su-Jin Park In vitro anti-reovirus activity of kuraridin isolated from Sophora flavescens against viral replication and hemagglutination Journal of Pharmacological Sciences Anti-reovirus activity Sophora flavescens Kuraridin Viral absorption and replication Hemagglutination |
| title | In vitro anti-reovirus activity of kuraridin isolated from Sophora flavescens against viral replication and hemagglutination |
| title_full | In vitro anti-reovirus activity of kuraridin isolated from Sophora flavescens against viral replication and hemagglutination |
| title_fullStr | In vitro anti-reovirus activity of kuraridin isolated from Sophora flavescens against viral replication and hemagglutination |
| title_full_unstemmed | In vitro anti-reovirus activity of kuraridin isolated from Sophora flavescens against viral replication and hemagglutination |
| title_short | In vitro anti-reovirus activity of kuraridin isolated from Sophora flavescens against viral replication and hemagglutination |
| title_sort | in vitro anti reovirus activity of kuraridin isolated from sophora flavescens against viral replication and hemagglutination |
| topic | Anti-reovirus activity Sophora flavescens Kuraridin Viral absorption and replication Hemagglutination |
| url | http://www.sciencedirect.com/science/article/pii/S1347861315000869 |
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