Regulatory Cross Talk Between SARS-CoV-2 Receptor Binding and Replication Machinery in the Human Host
We dissect the mechanism of SARS-CoV-2 in human lung host from the initial phase of receptor binding to viral replication machinery. Two independent lung protein interactome were constructed to reveal the signaling process on receptor activation and host protein hijacking machinery in the pathogenes...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-06-01
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| Series: | Frontiers in Physiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fphys.2020.00802/full |
| _version_ | 1831718524324675584 |
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| author | Shiek S. S. J. Ahmed Prabu Paramasivam Prabu Paramasivam Kamal Raj Vishal Kumar Ram Murugesan V. Ramakrishnan |
| author_facet | Shiek S. S. J. Ahmed Prabu Paramasivam Prabu Paramasivam Kamal Raj Vishal Kumar Ram Murugesan V. Ramakrishnan |
| author_sort | Shiek S. S. J. Ahmed |
| collection | DOAJ |
| description | We dissect the mechanism of SARS-CoV-2 in human lung host from the initial phase of receptor binding to viral replication machinery. Two independent lung protein interactome were constructed to reveal the signaling process on receptor activation and host protein hijacking machinery in the pathogenesis of virus. Further, we test the functional role of the hubs derived from the interactome. Most hubs proteins were differentially regulated on SARS-CoV-2 infection. Also, the proteins in viral replication hubs were related with cardiovascular disease, diabetes and hypertension confirming the vulnerability and severity of infection in the risk individual. Additionally, the hub proteins were closely linked with other viral infection, including MERS and HCoVs which suggest similar infection pattern in SARS-CoV-2. We identified five hubs that interconnect both networks that show the preparation of optimal environment in the host for viral replication process upon receptor attachment. Interestingly, we propose that seven potential miRNAs, targeting the intermediate phase that connects receptor and viral replication process a better choice as a drug for SARS-CoV-2. |
| first_indexed | 2024-12-21T01:17:33Z |
| format | Article |
| id | doaj.art-694cf93c94d6474ab8c9e1080622f112 |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-12-21T01:17:33Z |
| publishDate | 2020-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-694cf93c94d6474ab8c9e1080622f1122022-12-21T19:20:45ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2020-06-011110.3389/fphys.2020.00802560663Regulatory Cross Talk Between SARS-CoV-2 Receptor Binding and Replication Machinery in the Human HostShiek S. S. J. Ahmed0Prabu Paramasivam1Prabu Paramasivam2Kamal Raj3Vishal Kumar4Ram Murugesan5V. Ramakrishnan6Drug Discovery and Multi-Omics Laboratory, Faculty of Allied Health Sciences, Chettinad Academy of Research and Education, Kelambakkam, IndiaDepartment of Neurology, School of Medicine, University of New Mexico Health Sciences Center, Albuquerque, NM, United StatesMadras Diabetes Research Foundation, Chennai, IndiaMadras Diabetes Research Foundation, Chennai, IndiaDepartment of Immunology, YRG CARE, Centre for AIDS and Research Education, Chennai, IndiaDrug Discovery and Multi-Omics Laboratory, Faculty of Allied Health Sciences, Chettinad Academy of Research and Education, Kelambakkam, IndiaGenetics, Faculty of Allied Health Sciences, Chettinad Academy of Research and Education, Kelambakkam, IndiaWe dissect the mechanism of SARS-CoV-2 in human lung host from the initial phase of receptor binding to viral replication machinery. Two independent lung protein interactome were constructed to reveal the signaling process on receptor activation and host protein hijacking machinery in the pathogenesis of virus. Further, we test the functional role of the hubs derived from the interactome. Most hubs proteins were differentially regulated on SARS-CoV-2 infection. Also, the proteins in viral replication hubs were related with cardiovascular disease, diabetes and hypertension confirming the vulnerability and severity of infection in the risk individual. Additionally, the hub proteins were closely linked with other viral infection, including MERS and HCoVs which suggest similar infection pattern in SARS-CoV-2. We identified five hubs that interconnect both networks that show the preparation of optimal environment in the host for viral replication process upon receptor attachment. Interestingly, we propose that seven potential miRNAs, targeting the intermediate phase that connects receptor and viral replication process a better choice as a drug for SARS-CoV-2.https://www.frontiersin.org/article/10.3389/fphys.2020.00802/fullSARS-CoV-2Covid-19transcriptomesystems biologyhost mechanismmiRNA targets |
| spellingShingle | Shiek S. S. J. Ahmed Prabu Paramasivam Prabu Paramasivam Kamal Raj Vishal Kumar Ram Murugesan V. Ramakrishnan Regulatory Cross Talk Between SARS-CoV-2 Receptor Binding and Replication Machinery in the Human Host Frontiers in Physiology SARS-CoV-2 Covid-19 transcriptome systems biology host mechanism miRNA targets |
| title | Regulatory Cross Talk Between SARS-CoV-2 Receptor Binding and Replication Machinery in the Human Host |
| title_full | Regulatory Cross Talk Between SARS-CoV-2 Receptor Binding and Replication Machinery in the Human Host |
| title_fullStr | Regulatory Cross Talk Between SARS-CoV-2 Receptor Binding and Replication Machinery in the Human Host |
| title_full_unstemmed | Regulatory Cross Talk Between SARS-CoV-2 Receptor Binding and Replication Machinery in the Human Host |
| title_short | Regulatory Cross Talk Between SARS-CoV-2 Receptor Binding and Replication Machinery in the Human Host |
| title_sort | regulatory cross talk between sars cov 2 receptor binding and replication machinery in the human host |
| topic | SARS-CoV-2 Covid-19 transcriptome systems biology host mechanism miRNA targets |
| url | https://www.frontiersin.org/article/10.3389/fphys.2020.00802/full |
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