PhoR/PhoP two component regulatory system affects biocontrol capability of Bacillus subtilis NCD-2
The Bacillus subtilis strain NCD-2 is an important biocontrol agent against cotton verticillium wilt and cotton sore shin in the field, which are caused by Verticillium dahliae Kleb and Rhizoctonia solani Kuhn, respectively. A mutant of strain NCD-2, designated M216, with decreased antagonism to V....
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| Format: | Article |
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Sociedade Brasileira de Genética
2010-01-01
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| Series: | Genetics and Molecular Biology |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200022 |
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| author | Qinggang Guo Shezeng Li Xiuyun Lu Baoqing Li Ping Ma |
| author_facet | Qinggang Guo Shezeng Li Xiuyun Lu Baoqing Li Ping Ma |
| author_sort | Qinggang Guo |
| collection | DOAJ |
| description | The Bacillus subtilis strain NCD-2 is an important biocontrol agent against cotton verticillium wilt and cotton sore shin in the field, which are caused by Verticillium dahliae Kleb and Rhizoctonia solani Kuhn, respectively. A mutant of strain NCD-2, designated M216, with decreased antagonism to V. dahliae and R. solani, was selected by mini-Tn10 mutagenesis and in vitro virulence screening. The inserted gene in the mutant was cloned and identified as the phoR gene, which encodes a sensor kinase in the PhoP/PhoR two-component system. Compared to the wild-type strain, the APase activities of the mutant was decreased significantly when cultured in low phosphate medium, but no obvious difference was observed when cultured in high phosphate medium. The mutant also grew more slowly on organic phosphate agar and lost its phosphatidylcholine-solubilizing ability. The suppression of cotton seedling damping-off in vivo and colonization of the rhizosphere of cotton also decreased in the mutant strain when compared with the wild type strain. All of these characteristics could be partially restored by complementation of the phoR gene in the M216 mutant. |
| first_indexed | 2024-12-19T04:18:46Z |
| format | Article |
| id | doaj.art-694d91a3af2a47878d3a08f2b6cf55b6 |
| institution | Directory Open Access Journal |
| issn | 1415-4757 1678-4685 |
| language | English |
| last_indexed | 2024-12-19T04:18:46Z |
| publishDate | 2010-01-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-694d91a3af2a47878d3a08f2b6cf55b62022-12-21T20:36:13ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852010-01-01332333340PhoR/PhoP two component regulatory system affects biocontrol capability of Bacillus subtilis NCD-2Qinggang GuoShezeng LiXiuyun LuBaoqing LiPing MaThe Bacillus subtilis strain NCD-2 is an important biocontrol agent against cotton verticillium wilt and cotton sore shin in the field, which are caused by Verticillium dahliae Kleb and Rhizoctonia solani Kuhn, respectively. A mutant of strain NCD-2, designated M216, with decreased antagonism to V. dahliae and R. solani, was selected by mini-Tn10 mutagenesis and in vitro virulence screening. The inserted gene in the mutant was cloned and identified as the phoR gene, which encodes a sensor kinase in the PhoP/PhoR two-component system. Compared to the wild-type strain, the APase activities of the mutant was decreased significantly when cultured in low phosphate medium, but no obvious difference was observed when cultured in high phosphate medium. The mutant also grew more slowly on organic phosphate agar and lost its phosphatidylcholine-solubilizing ability. The suppression of cotton seedling damping-off in vivo and colonization of the rhizosphere of cotton also decreased in the mutant strain when compared with the wild type strain. All of these characteristics could be partially restored by complementation of the phoR gene in the M216 mutant.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200022Bacillus subtilisantagonismVerticillium dahliaemini-Tn10 |
| spellingShingle | Qinggang Guo Shezeng Li Xiuyun Lu Baoqing Li Ping Ma PhoR/PhoP two component regulatory system affects biocontrol capability of Bacillus subtilis NCD-2 Genetics and Molecular Biology Bacillus subtilis antagonism Verticillium dahliae mini-Tn10 |
| title | PhoR/PhoP two component regulatory system affects biocontrol capability of Bacillus subtilis NCD-2 |
| title_full | PhoR/PhoP two component regulatory system affects biocontrol capability of Bacillus subtilis NCD-2 |
| title_fullStr | PhoR/PhoP two component regulatory system affects biocontrol capability of Bacillus subtilis NCD-2 |
| title_full_unstemmed | PhoR/PhoP two component regulatory system affects biocontrol capability of Bacillus subtilis NCD-2 |
| title_short | PhoR/PhoP two component regulatory system affects biocontrol capability of Bacillus subtilis NCD-2 |
| title_sort | phor phop two component regulatory system affects biocontrol capability of bacillus subtilis ncd 2 |
| topic | Bacillus subtilis antagonism Verticillium dahliae mini-Tn10 |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200022 |
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