Marker-assisted selection of bacterial blight broad spectrum resistance genes Xa33 and Xa38 into CO43 in ICF3 generation

Bacterial Leaf Blight (BB) is one of the devastating diseases in Southern India. An elite rice variety, CO43 which is a medium duration variety (135 days) with average yield (6.3t/ha) and does not show resistance to Bacterial Leaf Blight (BB). Scientists had discovered several BB resistant genes w...

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Main Authors: R. Vennisa, N. Kumaravadivel and, A. Ramanathan
Format: Article
Language:English
Published: Indian Society of Plant Breeders 2018-10-01
Series:Electronic Journal of Plant Breeding
Subjects:
Online Access:http://ejplantbreeding.org/index.php/EJPB/article/view/2883
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author R. Vennisa
N. Kumaravadivel and
A. Ramanathan
author_facet R. Vennisa
N. Kumaravadivel and
A. Ramanathan
author_sort R. Vennisa
collection DOAJ
description Bacterial Leaf Blight (BB) is one of the devastating diseases in Southern India. An elite rice variety, CO43 which is a medium duration variety (135 days) with average yield (6.3t/ha) and does not show resistance to Bacterial Leaf Blight (BB). Scientists had discovered several BB resistant genes which are both dominant and recessive and is denoted as Xa and xa respectively. The current study is on the two genes Xa33 and Xa38 which are pyramided and is located on chromosome number 7 and 4 respectively. The rice genotypes FBR1-15 and PR114 were used as donors of Xa33, Xa38. The markers RMWR7.5 linked to Xa33 and Os04g53050-1 specific to Xa38 were validated in parents (CO43/ FBR1-15 and CO43/ PR114) and ICF3 populations to carry out Marker Assisted Selection (MAS). The segregating population of ICF3 were genotyped and the homozygous plants were subjected to artificial screening. The plants which showed resistance compared to CO43 are potential source of disease resistance which could be evaluated further and can be utilized for further breeding program.
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spelling doaj.art-694dd1d21c8142cda4aa55b334797cae2022-12-22T01:01:51ZengIndian Society of Plant BreedersElectronic Journal of Plant Breeding0975-928X2018-10-019397898410.5958/0975-928X.2018.00122.9Marker-assisted selection of bacterial blight broad spectrum resistance genes Xa33 and Xa38 into CO43 in ICF3 generationR. VennisaN. Kumaravadivel andA. RamanathanBacterial Leaf Blight (BB) is one of the devastating diseases in Southern India. An elite rice variety, CO43 which is a medium duration variety (135 days) with average yield (6.3t/ha) and does not show resistance to Bacterial Leaf Blight (BB). Scientists had discovered several BB resistant genes which are both dominant and recessive and is denoted as Xa and xa respectively. The current study is on the two genes Xa33 and Xa38 which are pyramided and is located on chromosome number 7 and 4 respectively. The rice genotypes FBR1-15 and PR114 were used as donors of Xa33, Xa38. The markers RMWR7.5 linked to Xa33 and Os04g53050-1 specific to Xa38 were validated in parents (CO43/ FBR1-15 and CO43/ PR114) and ICF3 populations to carry out Marker Assisted Selection (MAS). The segregating population of ICF3 were genotyped and the homozygous plants were subjected to artificial screening. The plants which showed resistance compared to CO43 are potential source of disease resistance which could be evaluated further and can be utilized for further breeding program.http://ejplantbreeding.org/index.php/EJPB/article/view/2883ricebacterial leaf blight (bb)xanthomonas oryzae pv. oryzae (xoo)foreground analysisartificial screening (clipping method).
spellingShingle R. Vennisa
N. Kumaravadivel and
A. Ramanathan
Marker-assisted selection of bacterial blight broad spectrum resistance genes Xa33 and Xa38 into CO43 in ICF3 generation
Electronic Journal of Plant Breeding
rice
bacterial leaf blight (bb)
xanthomonas oryzae pv. oryzae (xoo)
foreground analysis
artificial screening (clipping method).
title Marker-assisted selection of bacterial blight broad spectrum resistance genes Xa33 and Xa38 into CO43 in ICF3 generation
title_full Marker-assisted selection of bacterial blight broad spectrum resistance genes Xa33 and Xa38 into CO43 in ICF3 generation
title_fullStr Marker-assisted selection of bacterial blight broad spectrum resistance genes Xa33 and Xa38 into CO43 in ICF3 generation
title_full_unstemmed Marker-assisted selection of bacterial blight broad spectrum resistance genes Xa33 and Xa38 into CO43 in ICF3 generation
title_short Marker-assisted selection of bacterial blight broad spectrum resistance genes Xa33 and Xa38 into CO43 in ICF3 generation
title_sort marker assisted selection of bacterial blight broad spectrum resistance genes xa33 and xa38 into co43 in icf3 generation
topic rice
bacterial leaf blight (bb)
xanthomonas oryzae pv. oryzae (xoo)
foreground analysis
artificial screening (clipping method).
url http://ejplantbreeding.org/index.php/EJPB/article/view/2883
work_keys_str_mv AT rvennisa markerassistedselectionofbacterialblightbroadspectrumresistancegenesxa33andxa38intoco43inicf3generation
AT nkumaravadiveland markerassistedselectionofbacterialblightbroadspectrumresistancegenesxa33andxa38intoco43inicf3generation
AT aramanathan markerassistedselectionofbacterialblightbroadspectrumresistancegenesxa33andxa38intoco43inicf3generation