MicroRNA panels as diagnostic biomarkers for colorectal cancer: A systematic review and meta-analysis
BackgroundCirculating microRNAs (miRNA) have emerged as promising diagnostic biomarkers for several diseases, including cancer. However, the diagnostic accuracy of miRNA panels in colorectal cancer (CRC) remains inconsistent and there is still lack of meta-analyses to determine whether miRNA panels...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Medicine |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmed.2022.915226/full |
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| author | Daniel Sur Daniel Sur Shailesh Advani Shailesh Advani Dejana Braithwaite Dejana Braithwaite Dejana Braithwaite |
| author_facet | Daniel Sur Daniel Sur Shailesh Advani Shailesh Advani Dejana Braithwaite Dejana Braithwaite Dejana Braithwaite |
| author_sort | Daniel Sur |
| collection | DOAJ |
| description | BackgroundCirculating microRNAs (miRNA) have emerged as promising diagnostic biomarkers for several diseases, including cancer. However, the diagnostic accuracy of miRNA panels in colorectal cancer (CRC) remains inconsistent and there is still lack of meta-analyses to determine whether miRNA panels can serve as robust biomarkers for CRC diagnosis.MethodsThis study performed a systematic review and meta-analysis to evaluate the clinical utility of miRNA panels as potential biomarkers for the diagnosis of CRC. The investigation systematically searched PubMed, Medline, Web of Science, Cochrane Library, and Google Scholar (21-year span, between 2000 and 2021) to retrieve articles reporting the diagnostic role of miRNA panels in detecting CRC. Diagnostic meta-analysis of miRNA panels used diverse evaluation indicators, including sensitivity, specificity, Positive Likelihood Ratio (PLR), Negative Likelihood Ratio (NLR), Diagnostic Odds Ratio (DOR), and the area under the curve (AUC) values.ResultsAmong the 313 articles identified, 20 studies met the inclusion criteria. The pooled estimates of miRNA panels for the diagnosis of CRC were 0.85 (95% CI: 0.84–0.86), 0.79 (95% CI: 0.78–0.80), 4.06 (95% CI: 3.89–4.23), 0.20 (95% CI: 0.19–0.20), 22.50 (95% CI: 20.81–24.32) for sensitivity, specificity, PLR, NLR, and DOR, respectively. Moreover, the summary receiver operating characteristics (SROC) curve revealed an AUC value of 0.915 (95% CI: 0.914–0.916), suggesting an outstanding diagnostic accuracy for overall miRNA panels. Subgroup and meta-regression analyses demonstrated that miRNA panels have the highest diagnostic accuracy within serum samples, rather than in other sample-types – with a sensitivity, specificity, PLR, NLR, DOR, and AUC of 0.87, 0.86, 7.33, 0.13, 55.29, and 0.943, respectively. Sensitivity analysis revealed that DOR values did not differ markedly, which indicates that the meta-analysis had strong reliability. Furthermore, this study demonstrated no proof of publication bias for DOR values analyzed using Egger’s regression test (P > 0.05) and funnel plot. Interestingly, miR-15b, miR-21 and miR-31 presented the best diagnostic accuracy values for CRC with sensitivity, specificity, PLR, NLR, DOR, and AUC values of 0.95, 0.94, 17.19, 0.05, 324.81, and 0.948, respectively.ConclusionThis study’s findings indicated that miRNA panels, particularly serum-derived miRNA panels, can serve as powerful and promising biomarkers for early CRC screening.Systematic review registration[www.crd.york.ac.uk/prospero], identifier [CRD42021268172]. |
| first_indexed | 2024-04-11T22:54:27Z |
| format | Article |
| id | doaj.art-694e9f23786147f8a13aed5f82680fb7 |
| institution | Directory Open Access Journal |
| issn | 2296-858X |
| language | English |
| last_indexed | 2024-04-11T22:54:27Z |
| publishDate | 2022-11-01 |
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| series | Frontiers in Medicine |
| spelling | doaj.art-694e9f23786147f8a13aed5f82680fb72022-12-22T03:58:28ZengFrontiers Media S.A.Frontiers in Medicine2296-858X2022-11-01910.3389/fmed.2022.915226915226MicroRNA panels as diagnostic biomarkers for colorectal cancer: A systematic review and meta-analysisDaniel Sur0Daniel Sur1Shailesh Advani2Shailesh Advani3Dejana Braithwaite4Dejana Braithwaite5Dejana Braithwaite6Department of Medical Oncology, The Oncology Institute “Prof. Dr. Ion Chiricuţă” Cluj-Napoca, Cluj-Napoca, Romania11th Department of Medical Oncology, University of Medicine and Pharmacy “Iuliu Haţieganu”, Cluj-Napoca, RomaniaDepartment of Oncology, Georgetown University School of Medicine, Washington, DC, United StatesTerasaki Institute of Biomedical Innovation, Los Angeles, CA, United StatesDepartment of Epidemiology, University of Florida College of Public Health and Health Professions, Gainesville, FL, United StatesUniversity of Florida Health Cancer Center, Gainesville, FL, United StatesDepartment of Aging and Geriatric Research, University of Florida College of Medicine, Gainesville, FL, United StatesBackgroundCirculating microRNAs (miRNA) have emerged as promising diagnostic biomarkers for several diseases, including cancer. However, the diagnostic accuracy of miRNA panels in colorectal cancer (CRC) remains inconsistent and there is still lack of meta-analyses to determine whether miRNA panels can serve as robust biomarkers for CRC diagnosis.MethodsThis study performed a systematic review and meta-analysis to evaluate the clinical utility of miRNA panels as potential biomarkers for the diagnosis of CRC. The investigation systematically searched PubMed, Medline, Web of Science, Cochrane Library, and Google Scholar (21-year span, between 2000 and 2021) to retrieve articles reporting the diagnostic role of miRNA panels in detecting CRC. Diagnostic meta-analysis of miRNA panels used diverse evaluation indicators, including sensitivity, specificity, Positive Likelihood Ratio (PLR), Negative Likelihood Ratio (NLR), Diagnostic Odds Ratio (DOR), and the area under the curve (AUC) values.ResultsAmong the 313 articles identified, 20 studies met the inclusion criteria. The pooled estimates of miRNA panels for the diagnosis of CRC were 0.85 (95% CI: 0.84–0.86), 0.79 (95% CI: 0.78–0.80), 4.06 (95% CI: 3.89–4.23), 0.20 (95% CI: 0.19–0.20), 22.50 (95% CI: 20.81–24.32) for sensitivity, specificity, PLR, NLR, and DOR, respectively. Moreover, the summary receiver operating characteristics (SROC) curve revealed an AUC value of 0.915 (95% CI: 0.914–0.916), suggesting an outstanding diagnostic accuracy for overall miRNA panels. Subgroup and meta-regression analyses demonstrated that miRNA panels have the highest diagnostic accuracy within serum samples, rather than in other sample-types – with a sensitivity, specificity, PLR, NLR, DOR, and AUC of 0.87, 0.86, 7.33, 0.13, 55.29, and 0.943, respectively. Sensitivity analysis revealed that DOR values did not differ markedly, which indicates that the meta-analysis had strong reliability. Furthermore, this study demonstrated no proof of publication bias for DOR values analyzed using Egger’s regression test (P > 0.05) and funnel plot. Interestingly, miR-15b, miR-21 and miR-31 presented the best diagnostic accuracy values for CRC with sensitivity, specificity, PLR, NLR, DOR, and AUC values of 0.95, 0.94, 17.19, 0.05, 324.81, and 0.948, respectively.ConclusionThis study’s findings indicated that miRNA panels, particularly serum-derived miRNA panels, can serve as powerful and promising biomarkers for early CRC screening.Systematic review registration[www.crd.york.ac.uk/prospero], identifier [CRD42021268172].https://www.frontiersin.org/articles/10.3389/fmed.2022.915226/fullmiRNApanelscolorectal cancerdiagnosticbiomarker |
| spellingShingle | Daniel Sur Daniel Sur Shailesh Advani Shailesh Advani Dejana Braithwaite Dejana Braithwaite Dejana Braithwaite MicroRNA panels as diagnostic biomarkers for colorectal cancer: A systematic review and meta-analysis Frontiers in Medicine miRNA panels colorectal cancer diagnostic biomarker |
| title | MicroRNA panels as diagnostic biomarkers for colorectal cancer: A systematic review and meta-analysis |
| title_full | MicroRNA panels as diagnostic biomarkers for colorectal cancer: A systematic review and meta-analysis |
| title_fullStr | MicroRNA panels as diagnostic biomarkers for colorectal cancer: A systematic review and meta-analysis |
| title_full_unstemmed | MicroRNA panels as diagnostic biomarkers for colorectal cancer: A systematic review and meta-analysis |
| title_short | MicroRNA panels as diagnostic biomarkers for colorectal cancer: A systematic review and meta-analysis |
| title_sort | microrna panels as diagnostic biomarkers for colorectal cancer a systematic review and meta analysis |
| topic | miRNA panels colorectal cancer diagnostic biomarker |
| url | https://www.frontiersin.org/articles/10.3389/fmed.2022.915226/full |
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