Gene-editing techniques and their applications in livestock and beyond

Genetic modification enables modification of target genes or genome structure in livestock and experimental animals. These technologies have not only advanced bioscience but also improved agricultural productivity. To introduce a foreign transgene, the piggyBac transposon element/transposase system...

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Main Author: Tae Sub Park
Format: Article
Language:English
Published: Asian-Australasian Association of Animal Production Societies 2023-02-01
Series:Animal Bioscience
Subjects:
Online Access:http://www.animbiosci.org/upload/pdf/ab-22-0383.pdf
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author Tae Sub Park
author_facet Tae Sub Park
author_sort Tae Sub Park
collection DOAJ
description Genetic modification enables modification of target genes or genome structure in livestock and experimental animals. These technologies have not only advanced bioscience but also improved agricultural productivity. To introduce a foreign transgene, the piggyBac transposon element/transposase system could be used for production of transgenic animals and specific target protein-expressing animal cells. In addition, the clustered regularly interspaced short palindromic repeat-CRISPR associated protein 9 (CRISPR-Cas9) system have been utilized to generate chickens with knockout of G0/G1 switch gene 2 (G0S2) and myostatin, which are related to lipid deposition and muscle growth, respectively. These experimental chickens could be the invaluable genetic resources to investigate the regulatory pathways and mechanisms of improvement of economic traits such as fat quantity and growth. The gene-edited animals could also be applicable to the livestock industry.
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spelling doaj.art-694f2b6e6c414c5ca8c0fe3d12aa4a5a2023-02-06T01:13:59ZengAsian-Australasian Association of Animal Production SocietiesAnimal Bioscience2765-01892765-02352023-02-0136233333810.5713/ab.22.038324993Gene-editing techniques and their applications in livestock and beyondTae Sub Park0 Graduate School of International Agricultural Technology and Institute of Green-Bio Science and Technology, Seoul National University, Pyeongchang 25354, KoreaGenetic modification enables modification of target genes or genome structure in livestock and experimental animals. These technologies have not only advanced bioscience but also improved agricultural productivity. To introduce a foreign transgene, the piggyBac transposon element/transposase system could be used for production of transgenic animals and specific target protein-expressing animal cells. In addition, the clustered regularly interspaced short palindromic repeat-CRISPR associated protein 9 (CRISPR-Cas9) system have been utilized to generate chickens with knockout of G0/G1 switch gene 2 (G0S2) and myostatin, which are related to lipid deposition and muscle growth, respectively. These experimental chickens could be the invaluable genetic resources to investigate the regulatory pathways and mechanisms of improvement of economic traits such as fat quantity and growth. The gene-edited animals could also be applicable to the livestock industry.http://www.animbiosci.org/upload/pdf/ab-22-0383.pdfchickencrispr-cas9economic traitsgene-editingtransgenesis
spellingShingle Tae Sub Park
Gene-editing techniques and their applications in livestock and beyond
Animal Bioscience
chicken
crispr-cas9
economic traits
gene-editing
transgenesis
title Gene-editing techniques and their applications in livestock and beyond
title_full Gene-editing techniques and their applications in livestock and beyond
title_fullStr Gene-editing techniques and their applications in livestock and beyond
title_full_unstemmed Gene-editing techniques and their applications in livestock and beyond
title_short Gene-editing techniques and their applications in livestock and beyond
title_sort gene editing techniques and their applications in livestock and beyond
topic chicken
crispr-cas9
economic traits
gene-editing
transgenesis
url http://www.animbiosci.org/upload/pdf/ab-22-0383.pdf
work_keys_str_mv AT taesubpark geneeditingtechniquesandtheirapplicationsinlivestockandbeyond