Resistance to Acetylsalicylic Acid in Patients with Coronary Heart Disease Is the Result of Metabolic Activity of Platelets
Sensitivity to acetylsalicylic acid (ASA) is important in the treatment of patients with coronary heart disease (CHD) after coronary artery bypass grafting (CABG). Patients were divided into ASA sensitive (sASA) and ASA resistant (rASA) by the activity of platelet aggregation induced arachidonic aci...
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MDPI AG
2020-08-01
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| Series: | Pharmaceuticals |
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| Online Access: | https://www.mdpi.com/1424-8247/13/8/178 |
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| author | Yuriy I. Grinshtein Andrei A. Savchenko Aleksandra A. Kosinova Maxim D. Goncharov |
| author_facet | Yuriy I. Grinshtein Andrei A. Savchenko Aleksandra A. Kosinova Maxim D. Goncharov |
| author_sort | Yuriy I. Grinshtein |
| collection | DOAJ |
| description | Sensitivity to acetylsalicylic acid (ASA) is important in the treatment of patients with coronary heart disease (CHD) after coronary artery bypass grafting (CABG). Patients were divided into ASA sensitive (sASA) and ASA resistant (rASA) by the activity of platelet aggregation induced arachidonic acid (ARA) together with ASA. Induced platelet aggregation activity was studied in sASA and rASA patients with CHD before and after CABG. The level of synthesis of primary and secondary reactive oxygen species (ROS) by platelets was determined using chemiluminescent analysis. The activity of NAD- and NADP-dependent dehydrogenases in platelets was determined by the bioluminescent method. It was found that the aggregation activity of platelets depended on the sensitivity of CHD patients to ASA and decreased during postoperative ASA therapy. The most pronounced differences in metabolic parameters of platelets in sASA and rASA patients were detected by Nox2 activity. The synthesis of secondary ROS by platelets of CHD patients did not depend on the sensitivity of patients to ASA but increased during postoperative treatment with ASA. The activity of NAD(P)-dependent dehydrogenases in platelets did not differ in sASA and rASA patients with CHD. |
| first_indexed | 2024-03-10T18:02:11Z |
| format | Article |
| id | doaj.art-694f6ae05d04452a82b535d441a10ab4 |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-10T18:02:11Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj.art-694f6ae05d04452a82b535d441a10ab42023-11-20T08:45:11ZengMDPI AGPharmaceuticals1424-82472020-08-0113817810.3390/ph13080178Resistance to Acetylsalicylic Acid in Patients with Coronary Heart Disease Is the Result of Metabolic Activity of PlateletsYuriy I. Grinshtein0Andrei A. Savchenko1Aleksandra A. Kosinova2Maxim D. Goncharov3Therapeutic Department of Institute of Postgraduate Education, Krasnoyarsk State Medical University Named After Prof. V.F. Voyno-Yaseneckiy, 660125 Krasnoyarsk, RussiaTherapeutic Department of Institute of Postgraduate Education, Krasnoyarsk State Medical University Named After Prof. V.F. Voyno-Yaseneckiy, 660125 Krasnoyarsk, RussiaTherapeutic Department of Institute of Postgraduate Education, Krasnoyarsk State Medical University Named After Prof. V.F. Voyno-Yaseneckiy, 660125 Krasnoyarsk, RussiaTherapeutic Department of Institute of Postgraduate Education, Krasnoyarsk State Medical University Named After Prof. V.F. Voyno-Yaseneckiy, 660125 Krasnoyarsk, RussiaSensitivity to acetylsalicylic acid (ASA) is important in the treatment of patients with coronary heart disease (CHD) after coronary artery bypass grafting (CABG). Patients were divided into ASA sensitive (sASA) and ASA resistant (rASA) by the activity of platelet aggregation induced arachidonic acid (ARA) together with ASA. Induced platelet aggregation activity was studied in sASA and rASA patients with CHD before and after CABG. The level of synthesis of primary and secondary reactive oxygen species (ROS) by platelets was determined using chemiluminescent analysis. The activity of NAD- and NADP-dependent dehydrogenases in platelets was determined by the bioluminescent method. It was found that the aggregation activity of platelets depended on the sensitivity of CHD patients to ASA and decreased during postoperative ASA therapy. The most pronounced differences in metabolic parameters of platelets in sASA and rASA patients were detected by Nox2 activity. The synthesis of secondary ROS by platelets of CHD patients did not depend on the sensitivity of patients to ASA but increased during postoperative treatment with ASA. The activity of NAD(P)-dependent dehydrogenases in platelets did not differ in sASA and rASA patients with CHD.https://www.mdpi.com/1424-8247/13/8/178acetylsalicylic acid resistanceplateletsNox2reactive oxygen speciesdehydrogenase |
| spellingShingle | Yuriy I. Grinshtein Andrei A. Savchenko Aleksandra A. Kosinova Maxim D. Goncharov Resistance to Acetylsalicylic Acid in Patients with Coronary Heart Disease Is the Result of Metabolic Activity of Platelets Pharmaceuticals acetylsalicylic acid resistance platelets Nox2 reactive oxygen species dehydrogenase |
| title | Resistance to Acetylsalicylic Acid in Patients with Coronary Heart Disease Is the Result of Metabolic Activity of Platelets |
| title_full | Resistance to Acetylsalicylic Acid in Patients with Coronary Heart Disease Is the Result of Metabolic Activity of Platelets |
| title_fullStr | Resistance to Acetylsalicylic Acid in Patients with Coronary Heart Disease Is the Result of Metabolic Activity of Platelets |
| title_full_unstemmed | Resistance to Acetylsalicylic Acid in Patients with Coronary Heart Disease Is the Result of Metabolic Activity of Platelets |
| title_short | Resistance to Acetylsalicylic Acid in Patients with Coronary Heart Disease Is the Result of Metabolic Activity of Platelets |
| title_sort | resistance to acetylsalicylic acid in patients with coronary heart disease is the result of metabolic activity of platelets |
| topic | acetylsalicylic acid resistance platelets Nox2 reactive oxygen species dehydrogenase |
| url | https://www.mdpi.com/1424-8247/13/8/178 |
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