Insight into the Taxonomic and Functional Diversity of Bacterial Communities Inhabiting Blueberries in Portugal
<i>Vaccinium myrtillus</i> is a dwarf shrub of the <i>Ericaceae</i> family with a Palearctic distribution, associated with temperate and cold humid climates. It is widespread on the European continent; on the Iberian Peninsula it is located on Atlantic climate mountains and g...
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| Format: | Article |
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MDPI AG
2022-11-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/11/2193 |
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| author | Ana C. Gonçalves Fernando Sánchez-Juanes Sara Meirinho Luís R. Silva Gilberto Alves José David Flores-Félix |
| author_facet | Ana C. Gonçalves Fernando Sánchez-Juanes Sara Meirinho Luís R. Silva Gilberto Alves José David Flores-Félix |
| author_sort | Ana C. Gonçalves |
| collection | DOAJ |
| description | <i>Vaccinium myrtillus</i> is a dwarf shrub of the <i>Ericaceae</i> family with a Palearctic distribution, associated with temperate and cold humid climates. It is widespread on the European continent; on the Iberian Peninsula it is located on Atlantic climate mountains and glacial relicts. In Portugal, we find scattered and interesting populations; however, the majority of them are threatened by climate change and wildfires. Given that, the objective of this study is to determine the rhizospheric and root bacterial communities of this plant in the southernmost regions, and, consequently, its potential range and ability to be used as a biofertilizer. In this work, metabarcoding of 16S rRNA gene showed that the endophytic bacterial diversity is dependent on the plant and selected by it according to the observed alpha and beta diversity. Moreover, a culturomic approach allowed 142 different strains to be isolated, some of them being putative new species. Additionally, some strains belonging to the genera <i>Bacillus, Paenibacillus, Pseudomonas, Paraburkholderia</i>, and <i>Caballeronia</i> showed significant potential to be applied as multifunctional biofertilizers since they present good plant growth-promoting (PGP) mechanisms, high colonization capacities, and an increase in vegetative parameters in blueberry and tomato plants. |
| first_indexed | 2024-03-09T18:49:56Z |
| format | Article |
| id | doaj.art-69511bee1d1946d58b387951f99fcb3b |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T18:49:56Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-69511bee1d1946d58b387951f99fcb3b2023-11-24T05:57:13ZengMDPI AGMicroorganisms2076-26072022-11-011011219310.3390/microorganisms10112193Insight into the Taxonomic and Functional Diversity of Bacterial Communities Inhabiting Blueberries in PortugalAna C. Gonçalves0Fernando Sánchez-Juanes1Sara Meirinho2Luís R. Silva3Gilberto Alves4José David Flores-Félix5CICS–UBI—Health Sciences Research Centre, University of Beira Interior, 6201-506 Covilhã, PortugalInstituto de Investigación Biomédica de Salamanca (IBSAL), Complejo Asistencial Universitario de Salamanca, Universidad de Salamanca, CSIC, 37007 Salamanca, SpainCICS–UBI—Health Sciences Research Centre, University of Beira Interior, 6201-506 Covilhã, PortugalCICS–UBI—Health Sciences Research Centre, University of Beira Interior, 6201-506 Covilhã, PortugalCICS–UBI—Health Sciences Research Centre, University of Beira Interior, 6201-506 Covilhã, PortugalCICS–UBI—Health Sciences Research Centre, University of Beira Interior, 6201-506 Covilhã, Portugal<i>Vaccinium myrtillus</i> is a dwarf shrub of the <i>Ericaceae</i> family with a Palearctic distribution, associated with temperate and cold humid climates. It is widespread on the European continent; on the Iberian Peninsula it is located on Atlantic climate mountains and glacial relicts. In Portugal, we find scattered and interesting populations; however, the majority of them are threatened by climate change and wildfires. Given that, the objective of this study is to determine the rhizospheric and root bacterial communities of this plant in the southernmost regions, and, consequently, its potential range and ability to be used as a biofertilizer. In this work, metabarcoding of 16S rRNA gene showed that the endophytic bacterial diversity is dependent on the plant and selected by it according to the observed alpha and beta diversity. Moreover, a culturomic approach allowed 142 different strains to be isolated, some of them being putative new species. Additionally, some strains belonging to the genera <i>Bacillus, Paenibacillus, Pseudomonas, Paraburkholderia</i>, and <i>Caballeronia</i> showed significant potential to be applied as multifunctional biofertilizers since they present good plant growth-promoting (PGP) mechanisms, high colonization capacities, and an increase in vegetative parameters in blueberry and tomato plants.https://www.mdpi.com/2076-2607/10/11/2193<i>Vaccinium</i>blueberrybacterial diversityplant growth-promoting bacteriaPortugal |
| spellingShingle | Ana C. Gonçalves Fernando Sánchez-Juanes Sara Meirinho Luís R. Silva Gilberto Alves José David Flores-Félix Insight into the Taxonomic and Functional Diversity of Bacterial Communities Inhabiting Blueberries in Portugal Microorganisms <i>Vaccinium</i> blueberry bacterial diversity plant growth-promoting bacteria Portugal |
| title | Insight into the Taxonomic and Functional Diversity of Bacterial Communities Inhabiting Blueberries in Portugal |
| title_full | Insight into the Taxonomic and Functional Diversity of Bacterial Communities Inhabiting Blueberries in Portugal |
| title_fullStr | Insight into the Taxonomic and Functional Diversity of Bacterial Communities Inhabiting Blueberries in Portugal |
| title_full_unstemmed | Insight into the Taxonomic and Functional Diversity of Bacterial Communities Inhabiting Blueberries in Portugal |
| title_short | Insight into the Taxonomic and Functional Diversity of Bacterial Communities Inhabiting Blueberries in Portugal |
| title_sort | insight into the taxonomic and functional diversity of bacterial communities inhabiting blueberries in portugal |
| topic | <i>Vaccinium</i> blueberry bacterial diversity plant growth-promoting bacteria Portugal |
| url | https://www.mdpi.com/2076-2607/10/11/2193 |
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