| Summary: | This study aims to determine the species composition and density of colony-forming units (CFU) of entomopathogenic fungi (EPF) in leaf litter at different depths of the top layer of forest soils depending on the type of forest (coniferous, deciduous and mixed forest), and the date of sampling (spring, autumn). In each type of forest, leaf litter and soil were collected using a soil stick from four depths of soil: 0–5, 5–10, 10–15 and 15–20 cm. Entomopathogenic fungi were isolated by a soil or litter dilution plating method on a selective medium. Four fungal genera were found: <i>Beauveria</i> spp., <i>Cordyceps</i> spp., <i>Metarhizium</i> spp., and <i>Lecanicillium</i> spp. The density of EPF was usually higher in leaf litter than in the layers of soil below, and the most frequently isolated species from both environments were <i>Beauveria</i> spp. among soil samples from all forest types; <i>Beauveria</i> spp. were most abundant in the top layer (0–5 cm), and their density of CFUs gradually decreased deeper into the soil profile.
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