Isolation, Structure Determination, and Cytotoxic Activity of Steroid Compound from The Stem Bark of Aglaia cucullata (Meliaceae)
Steroids are one of the secondary metabolite groups that are abundant in many organisms. In plants, this type of compound is called phytosterols. Phytosterols have been widely known to show many potential bioactivities such as anti-inflammatory, induced apoptosis, cytotoxic, anti-diabetic, angiogeni...
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| Format: | Article |
| Language: | English |
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Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University
2023-09-01
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| Series: | Jurnal Kimia Sains dan Aplikasi |
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| Online Access: | https://ejournal.undip.ac.id/index.php/ksa/article/view/56368 |
| _version_ | 1797655986426609664 |
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| author | Kindi Farabi Desi Harneti Nurlelasari Nurlelasari Tri Mayanti Rani Maharani Unang Supratman |
| author_facet | Kindi Farabi Desi Harneti Nurlelasari Nurlelasari Tri Mayanti Rani Maharani Unang Supratman |
| author_sort | Kindi Farabi |
| collection | DOAJ |
| description | Steroids are one of the secondary metabolite groups that are abundant in many organisms. In plants, this type of compound is called phytosterols. Phytosterols have been widely known to show many potential bioactivities such as anti-inflammatory, induced apoptosis, cytotoxic, anti-diabetic, angiogenic, and antioxidant. One of the sources of phytosterol compounds is the genus Aglaia. As the largest genus in the Meliaceae family, the genus Aglaia contains many bioactive compounds, including steroids. This research reported the isolation, structural determination, and cytotoxic activity of steroid compounds from the stem bark of Aglaia cucullata, one of the members of the Aglaia genus. Pure isolated steroid was obtained after maceration of dried stem bark with ethanol and partitioned based on difference polarity, continued by column chromatography. Spectroscopic methods, including HRMS, FTIR, 1D and 2D NMR, were used for structural determination. The compound structure identified as stigmast-5-en-3β-ol-3β-oleate was first isolated from this species. MCF-7 breast cancer cell, B16-F10 melanoma cell, and CV-1 normal fibroblast kidney cell were used to evaluate its cytotoxicity. Stigmast-5-en-3β-ol-3β-oleate displayed low cytotoxicity against those two cancer cells and a normal cell. |
| first_indexed | 2024-03-11T17:22:38Z |
| format | Article |
| id | doaj.art-6956a19d39ef493d86ea097b71ef4618 |
| institution | Directory Open Access Journal |
| issn | 1410-8917 2597-9914 |
| language | English |
| last_indexed | 2024-03-11T17:22:38Z |
| publishDate | 2023-09-01 |
| publisher | Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University |
| record_format | Article |
| series | Jurnal Kimia Sains dan Aplikasi |
| spelling | doaj.art-6956a19d39ef493d86ea097b71ef46182023-10-19T10:05:14ZengChemistry Department, Faculty of Sciences and Mathematics, Diponegoro UniversityJurnal Kimia Sains dan Aplikasi1410-89172597-99142023-09-0126621722310.14710/jksa.26.6.217-22322764Isolation, Structure Determination, and Cytotoxic Activity of Steroid Compound from The Stem Bark of Aglaia cucullata (Meliaceae)Kindi Farabi0https://orcid.org/0000-0001-5552-3827Desi Harneti1https://orcid.org/0000-0002-8120-7892Nurlelasari Nurlelasari2https://orcid.org/0000-0002-9317-2607Tri Mayanti3https://orcid.org/0000-0003-1628-6288Rani Maharani4https://orcid.org/0000-0002-4832-7227Unang Supratman5https://orcid.org/0000-0003-1104-2321Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jatinangor 45363, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jatinangor 45363, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jatinangor 45363, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jatinangor 45363, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jatinangor 45363, IndonesiaCentral Laboratory, Universitas Padjadjaran, Jatinangor 45363, IndonesiaSteroids are one of the secondary metabolite groups that are abundant in many organisms. In plants, this type of compound is called phytosterols. Phytosterols have been widely known to show many potential bioactivities such as anti-inflammatory, induced apoptosis, cytotoxic, anti-diabetic, angiogenic, and antioxidant. One of the sources of phytosterol compounds is the genus Aglaia. As the largest genus in the Meliaceae family, the genus Aglaia contains many bioactive compounds, including steroids. This research reported the isolation, structural determination, and cytotoxic activity of steroid compounds from the stem bark of Aglaia cucullata, one of the members of the Aglaia genus. Pure isolated steroid was obtained after maceration of dried stem bark with ethanol and partitioned based on difference polarity, continued by column chromatography. Spectroscopic methods, including HRMS, FTIR, 1D and 2D NMR, were used for structural determination. The compound structure identified as stigmast-5-en-3β-ol-3β-oleate was first isolated from this species. MCF-7 breast cancer cell, B16-F10 melanoma cell, and CV-1 normal fibroblast kidney cell were used to evaluate its cytotoxicity. Stigmast-5-en-3β-ol-3β-oleate displayed low cytotoxicity against those two cancer cells and a normal cell.https://ejournal.undip.ac.id/index.php/ksa/article/view/56368steroidaglaiaaglaia cucullatacytotoxic activity |
| spellingShingle | Kindi Farabi Desi Harneti Nurlelasari Nurlelasari Tri Mayanti Rani Maharani Unang Supratman Isolation, Structure Determination, and Cytotoxic Activity of Steroid Compound from The Stem Bark of Aglaia cucullata (Meliaceae) Jurnal Kimia Sains dan Aplikasi steroid aglaia aglaia cucullata cytotoxic activity |
| title | Isolation, Structure Determination, and Cytotoxic Activity of Steroid Compound from The Stem Bark of Aglaia cucullata (Meliaceae) |
| title_full | Isolation, Structure Determination, and Cytotoxic Activity of Steroid Compound from The Stem Bark of Aglaia cucullata (Meliaceae) |
| title_fullStr | Isolation, Structure Determination, and Cytotoxic Activity of Steroid Compound from The Stem Bark of Aglaia cucullata (Meliaceae) |
| title_full_unstemmed | Isolation, Structure Determination, and Cytotoxic Activity of Steroid Compound from The Stem Bark of Aglaia cucullata (Meliaceae) |
| title_short | Isolation, Structure Determination, and Cytotoxic Activity of Steroid Compound from The Stem Bark of Aglaia cucullata (Meliaceae) |
| title_sort | isolation structure determination and cytotoxic activity of steroid compound from the stem bark of aglaia cucullata meliaceae |
| topic | steroid aglaia aglaia cucullata cytotoxic activity |
| url | https://ejournal.undip.ac.id/index.php/ksa/article/view/56368 |
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