Bioactivities, phenolic compounds and in-vitro propagation of Lippia citriodora Kunth cultivated in Egypt
The aqueous and alcoholic extracts of the fresh aerial parts of Lippia citriodora Kunth, family Verbenaceae, cultivated in Egypt, exhibited variable antiinflammatory, antipyretic, analgesic and antioxidant properties. Three phenolic compounds; two phenolic acids, dihydrocaffeic acid (1) and 4-hydrox...
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| Format: | Article |
| Language: | English |
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Faculty of Pharmacy, Cairo University
2012-06-01
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| Series: | Bulletin of Faculty of Pharmacy Cairo University |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1110093112000038 |
| _version_ | 1797964824437587968 |
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| author | Seham S. El-Hawary Miriam F. Yousif Amira A. Abdel Motaal Lamia M. Abd-Hameed |
| author_facet | Seham S. El-Hawary Miriam F. Yousif Amira A. Abdel Motaal Lamia M. Abd-Hameed |
| author_sort | Seham S. El-Hawary |
| collection | DOAJ |
| description | The aqueous and alcoholic extracts of the fresh aerial parts of Lippia citriodora Kunth, family Verbenaceae, cultivated in Egypt, exhibited variable antiinflammatory, antipyretic, analgesic and antioxidant properties. Three phenolic compounds; two phenolic acids, dihydrocaffeic acid (1) and 4-hydroxycinnamic acid (3) and a flavonoid glycoside, luteolin-7-O-glucoside (2), were isolated and identified from the ethyl acetate fraction of the plant. The structures of the isolated compounds were elucidated on the basis of spectral analysis. The effects of explant type, method of sterilization and growth regulators on the in-vitro callus formation of L. citriodora were studied. Shoot tips and leaf explants (cut in the midrib region) sterilized by soaking in 0.2% mercuric chloride for 5 min, then washed twice with sterilized distilled water gave callus, on Murashige and Skoog (MS) medium impregnated with 4 mg/L 6-benzyl amino purine (BAP). Spectrophotometric estimation of the total flavonoids showed that the fresh in-vitro formed callus contained 68.4% of the total flavonoids of the fresh aerial parts of the conventional plant. |
| first_indexed | 2024-04-11T01:50:57Z |
| format | Article |
| id | doaj.art-6958f71971f24fe8b9e1b7688558dab0 |
| institution | Directory Open Access Journal |
| issn | 1110-0931 |
| language | English |
| last_indexed | 2024-04-11T01:50:57Z |
| publishDate | 2012-06-01 |
| publisher | Faculty of Pharmacy, Cairo University |
| record_format | Article |
| series | Bulletin of Faculty of Pharmacy Cairo University |
| spelling | doaj.art-6958f71971f24fe8b9e1b7688558dab02023-01-03T06:27:44ZengFaculty of Pharmacy, Cairo UniversityBulletin of Faculty of Pharmacy Cairo University1110-09312012-06-015011610.1016/j.bfopcu.2011.12.001Bioactivities, phenolic compounds and in-vitro propagation of Lippia citriodora Kunth cultivated in EgyptSeham S. El-Hawary0Miriam F. Yousif1Amira A. Abdel Motaal2Lamia M. Abd-Hameed3Pharmacognosy Department, Faculty of Pharmacy, Cairo University, Kasr-El-Ainy St., Cairo 11562, EgyptPharmacognosy Department, Faculty of Pharmacy, Cairo University, Kasr-El-Ainy St., Cairo 11562, EgyptPharmacognosy Department, Faculty of Pharmacy, Cairo University, Kasr-El-Ainy St., Cairo 11562, EgyptDefence Industry Medical Center, Ismail Kamel Street, East Helwan, EgyptThe aqueous and alcoholic extracts of the fresh aerial parts of Lippia citriodora Kunth, family Verbenaceae, cultivated in Egypt, exhibited variable antiinflammatory, antipyretic, analgesic and antioxidant properties. Three phenolic compounds; two phenolic acids, dihydrocaffeic acid (1) and 4-hydroxycinnamic acid (3) and a flavonoid glycoside, luteolin-7-O-glucoside (2), were isolated and identified from the ethyl acetate fraction of the plant. The structures of the isolated compounds were elucidated on the basis of spectral analysis. The effects of explant type, method of sterilization and growth regulators on the in-vitro callus formation of L. citriodora were studied. Shoot tips and leaf explants (cut in the midrib region) sterilized by soaking in 0.2% mercuric chloride for 5 min, then washed twice with sterilized distilled water gave callus, on Murashige and Skoog (MS) medium impregnated with 4 mg/L 6-benzyl amino purine (BAP). Spectrophotometric estimation of the total flavonoids showed that the fresh in-vitro formed callus contained 68.4% of the total flavonoids of the fresh aerial parts of the conventional plant.http://www.sciencedirect.com/science/article/pii/S1110093112000038Lippia citriodoraAntiinflammatoryDihydrocaffeic acidIn-vitro propagation |
| spellingShingle | Seham S. El-Hawary Miriam F. Yousif Amira A. Abdel Motaal Lamia M. Abd-Hameed Bioactivities, phenolic compounds and in-vitro propagation of Lippia citriodora Kunth cultivated in Egypt Bulletin of Faculty of Pharmacy Cairo University Lippia citriodora Antiinflammatory Dihydrocaffeic acid In-vitro propagation |
| title | Bioactivities, phenolic compounds and in-vitro propagation of Lippia citriodora Kunth cultivated in Egypt |
| title_full | Bioactivities, phenolic compounds and in-vitro propagation of Lippia citriodora Kunth cultivated in Egypt |
| title_fullStr | Bioactivities, phenolic compounds and in-vitro propagation of Lippia citriodora Kunth cultivated in Egypt |
| title_full_unstemmed | Bioactivities, phenolic compounds and in-vitro propagation of Lippia citriodora Kunth cultivated in Egypt |
| title_short | Bioactivities, phenolic compounds and in-vitro propagation of Lippia citriodora Kunth cultivated in Egypt |
| title_sort | bioactivities phenolic compounds and in vitro propagation of lippia citriodora kunth cultivated in egypt |
| topic | Lippia citriodora Antiinflammatory Dihydrocaffeic acid In-vitro propagation |
| url | http://www.sciencedirect.com/science/article/pii/S1110093112000038 |
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