Epigenetic manipulation for secondary metabolite activation in endophytic fungi: current progress and future directions
ABSTRACTFungal endophytes have emerged as a promising source of secondary metabolites with significant potential for various applications in the field of biomedicine. The biosynthetic gene clusters of endophytic fungi are responsible for encoding several enzymes and transcriptional factors that are...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-10-01
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| Series: | Mycology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21501203.2023.2241486 |
| _version_ | 1797349223103987712 |
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| author | Ashish Verma Harshita Tiwari Swati Singh Priyamvada Gupta Nilesh Rai Santosh Kumar Singh Bhim Pratap Singh Sombir Rao Vibhav Gautam |
| author_facet | Ashish Verma Harshita Tiwari Swati Singh Priyamvada Gupta Nilesh Rai Santosh Kumar Singh Bhim Pratap Singh Sombir Rao Vibhav Gautam |
| author_sort | Ashish Verma |
| collection | DOAJ |
| description | ABSTRACTFungal endophytes have emerged as a promising source of secondary metabolites with significant potential for various applications in the field of biomedicine. The biosynthetic gene clusters of endophytic fungi are responsible for encoding several enzymes and transcriptional factors that are involved in the biosynthesis of secondary metabolites. The investigation of fungal metabolic potential at genetic level faces certain challenges, including the synthesis of appropriate amounts of chemicals, and loss of the ability of fungal endophytes to produce secondary metabolites in an artificial culture medium. Therefore, there is a need to delve deeper into the field of fungal genomics and transcriptomics to explore the potential of fungal endophytes in generating secondary metabolites governed by biosynthetic gene clusters. The silent biosynthetic gene clusters can be activated by modulating the chromatin structure using chemical compounds. Epigenetic modification plays a significant role by inducing cryptic gene responsible for the production of secondary metabolites using DNA methyl transferase and histone deacetylase. CRISPR-Cas9-based genome editing emerges an effective tool to enhance the production of desired metabolites by modulating gene expression. This review primarily focuses on the significance of epigenetic elicitors and their capacity to boost the production of secondary metabolites from endophytes. This article holds the potential to rejuvenate the drug discovery pipeline by introducing new chemical compounds. |
| first_indexed | 2024-03-08T12:27:14Z |
| format | Article |
| id | doaj.art-695ad317b4354089bbaa55728eeaf470 |
| institution | Directory Open Access Journal |
| issn | 2150-1203 2150-1211 |
| language | English |
| last_indexed | 2024-03-08T12:27:14Z |
| publishDate | 2023-10-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Mycology |
| spelling | doaj.art-695ad317b4354089bbaa55728eeaf4702024-01-22T07:21:34ZengTaylor & Francis GroupMycology2150-12032150-12112023-10-0114427529110.1080/21501203.2023.2241486Epigenetic manipulation for secondary metabolite activation in endophytic fungi: current progress and future directionsAshish Verma0Harshita Tiwari1Swati Singh2Priyamvada Gupta3Nilesh Rai4Santosh Kumar Singh5Bhim Pratap Singh6Sombir Rao7Vibhav Gautam8Centre of Experimental Medicine and Surgery, Institute of Medical Sciences, Banaras Hindu University, Varanasi, IndiaCentre of Experimental Medicine and Surgery, Institute of Medical Sciences, Banaras Hindu University, Varanasi, IndiaCentre of Experimental Medicine and Surgery, Institute of Medical Sciences, Banaras Hindu University, Varanasi, IndiaCentre of Experimental Medicine and Surgery, Institute of Medical Sciences, Banaras Hindu University, Varanasi, IndiaCentre of Experimental Medicine and Surgery, Institute of Medical Sciences, Banaras Hindu University, Varanasi, IndiaCentre of Experimental Medicine and Surgery, Institute of Medical Sciences, Banaras Hindu University, Varanasi, IndiaDepartment of Agriculture & Environmental Sciences (AES), National Institute of Food Technology Entrepreneurship & Management (NIFTEM), Sonepat, IndiaPlant Breeding and Genetics Section, School of Integrative Plant Science, Cornell University, Ithaca, NY, USACentre of Experimental Medicine and Surgery, Institute of Medical Sciences, Banaras Hindu University, Varanasi, IndiaABSTRACTFungal endophytes have emerged as a promising source of secondary metabolites with significant potential for various applications in the field of biomedicine. The biosynthetic gene clusters of endophytic fungi are responsible for encoding several enzymes and transcriptional factors that are involved in the biosynthesis of secondary metabolites. The investigation of fungal metabolic potential at genetic level faces certain challenges, including the synthesis of appropriate amounts of chemicals, and loss of the ability of fungal endophytes to produce secondary metabolites in an artificial culture medium. Therefore, there is a need to delve deeper into the field of fungal genomics and transcriptomics to explore the potential of fungal endophytes in generating secondary metabolites governed by biosynthetic gene clusters. The silent biosynthetic gene clusters can be activated by modulating the chromatin structure using chemical compounds. Epigenetic modification plays a significant role by inducing cryptic gene responsible for the production of secondary metabolites using DNA methyl transferase and histone deacetylase. CRISPR-Cas9-based genome editing emerges an effective tool to enhance the production of desired metabolites by modulating gene expression. This review primarily focuses on the significance of epigenetic elicitors and their capacity to boost the production of secondary metabolites from endophytes. This article holds the potential to rejuvenate the drug discovery pipeline by introducing new chemical compounds.https://www.tandfonline.com/doi/10.1080/21501203.2023.2241486Fungal endophyteschromatinhistone modificationbioactive compoundssecondary metabolites |
| spellingShingle | Ashish Verma Harshita Tiwari Swati Singh Priyamvada Gupta Nilesh Rai Santosh Kumar Singh Bhim Pratap Singh Sombir Rao Vibhav Gautam Epigenetic manipulation for secondary metabolite activation in endophytic fungi: current progress and future directions Mycology Fungal endophytes chromatin histone modification bioactive compounds secondary metabolites |
| title | Epigenetic manipulation for secondary metabolite activation in endophytic fungi: current progress and future directions |
| title_full | Epigenetic manipulation for secondary metabolite activation in endophytic fungi: current progress and future directions |
| title_fullStr | Epigenetic manipulation for secondary metabolite activation in endophytic fungi: current progress and future directions |
| title_full_unstemmed | Epigenetic manipulation for secondary metabolite activation in endophytic fungi: current progress and future directions |
| title_short | Epigenetic manipulation for secondary metabolite activation in endophytic fungi: current progress and future directions |
| title_sort | epigenetic manipulation for secondary metabolite activation in endophytic fungi current progress and future directions |
| topic | Fungal endophytes chromatin histone modification bioactive compounds secondary metabolites |
| url | https://www.tandfonline.com/doi/10.1080/21501203.2023.2241486 |
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