Lineage tracing of T cell differentiation from T-iPSC by 2D feeder-free culture and 3D organoid culture
IntroductionT cells induced from induced pluripotent stem cells(iPSCs) derived from antigen-specific T cells (T-iPS-T cells) are an attractive tool for T cell immunotherapy. The induction of cytotoxic T-iPS-T cells is well established in feeder-free condition for the aim of off-the-shelf production,...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-12-01
|
| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1303713/full |
| _version_ | 1797390144633831424 |
|---|---|
| author | Yoshitaka Ishiguro Yoshitaka Ishiguro Yoshitaka Ishiguro Shoichi Iriguchi Shoichi Iriguchi Shinya Asano Tokuyuki Shinohara Tokuyuki Shinohara Sara Shiina Sara Shiina Suguru Arima Suguru Arima Yoshiaki Kassai Yoshiaki Kassai Yoshiharu Sakai Kazutaka Obama Shin Kaneko Shin Kaneko |
| author_facet | Yoshitaka Ishiguro Yoshitaka Ishiguro Yoshitaka Ishiguro Shoichi Iriguchi Shoichi Iriguchi Shinya Asano Tokuyuki Shinohara Tokuyuki Shinohara Sara Shiina Sara Shiina Suguru Arima Suguru Arima Yoshiaki Kassai Yoshiaki Kassai Yoshiharu Sakai Kazutaka Obama Shin Kaneko Shin Kaneko |
| author_sort | Yoshitaka Ishiguro |
| collection | DOAJ |
| description | IntroductionT cells induced from induced pluripotent stem cells(iPSCs) derived from antigen-specific T cells (T-iPS-T cells) are an attractive tool for T cell immunotherapy. The induction of cytotoxic T-iPS-T cells is well established in feeder-free condition for the aim of off-the-shelf production, however, the induction of helper T-iPS-T cells remains challenging.MethodsWe analyzed T-iPS-T cells matured in 3D organoid culture at different steps in the culture process at the single-cell level. T-iPS-T cell datasets were merged with an available human thymocyte dataset based in single-cell RNA sequencing (scRNA-seq). Particularly, we searched for genes crucial for generation CD4+ T-iPS-T cells by comparing T-iPS-T cells established in 2D feeder-free or 3D organoid culture.ResultsThe scRNA-seq data indicated that T-iPS-T cells are similar to T cells transitioning to human thymocytes, with SELENOW, GIMAP4, 7, SATB1, SALMF1, IL7R, SYTL2, S100A11, STAT1, IFITM1, LZTFL1 and SOX4 identified as candidate genes for the 2D feeder-free induction of CD4+ T-iPS-T cells.DiscussionThis study provides single cell transcriptome datasets of iPS-T cells and leads to further analysis for CD4+ T cell generation from T-iPSCs. |
| first_indexed | 2024-03-08T23:07:25Z |
| format | Article |
| id | doaj.art-695b9dbb13ac4db38ab7f83e79b03c2d |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-03-08T23:07:25Z |
| publishDate | 2023-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-695b9dbb13ac4db38ab7f83e79b03c2d2023-12-15T11:03:45ZengFrontiers Media S.A.Frontiers in Immunology1664-32242023-12-011410.3389/fimmu.2023.13037131303713Lineage tracing of T cell differentiation from T-iPSC by 2D feeder-free culture and 3D organoid cultureYoshitaka Ishiguro0Yoshitaka Ishiguro1Yoshitaka Ishiguro2Shoichi Iriguchi3Shoichi Iriguchi4Shinya Asano5Tokuyuki Shinohara6Tokuyuki Shinohara7Sara Shiina8Sara Shiina9Suguru Arima10Suguru Arima11Yoshiaki Kassai12Yoshiaki Kassai13Yoshiharu Sakai14Kazutaka Obama15Shin Kaneko16Shin Kaneko17Shin Kaneko Laboratory, Department of Cell Growth and Differentiation, Kyoto University, Shogoin-Kawahara-cho, Sakyo-ku, Kyoto, JapanTakeda-CiRA Joint Program (T-CiRA), Fujisawa, JapanDepartment of Surgery, Graduate School of Medicine, Kyoto University, Shogoin-Kawahara-cho, Sakyo-ku, Kyoto, JapanShin Kaneko Laboratory, Department of Cell Growth and Differentiation, Kyoto University, Shogoin-Kawahara-cho, Sakyo-ku, Kyoto, JapanTakeda-CiRA Joint Program (T-CiRA), Fujisawa, JapanAxcelead Drug Discovery Partners, Inc., Fujisawa, JapanTakeda-CiRA Joint Program (T-CiRA), Fujisawa, JapanT-CiRA Discovery and Innovation, Takeda Pharmaceutical Company, Fujisawa, JapanShin Kaneko Laboratory, Department of Cell Growth and Differentiation, Kyoto University, Shogoin-Kawahara-cho, Sakyo-ku, Kyoto, JapanTakeda-CiRA Joint Program (T-CiRA), Fujisawa, JapanTakeda-CiRA Joint Program (T-CiRA), Fujisawa, JapanT-CiRA Discovery and Innovation, Takeda Pharmaceutical Company, Fujisawa, JapanTakeda-CiRA Joint Program (T-CiRA), Fujisawa, JapanT-CiRA Discovery and Innovation, Takeda Pharmaceutical Company, Fujisawa, JapanDepartment of Surgery, Osaka Red Cross Hospital, Fudegasaki-cho, Tennoji-ku, Osaka, JapanDepartment of Surgery, Graduate School of Medicine, Kyoto University, Shogoin-Kawahara-cho, Sakyo-ku, Kyoto, JapanShin Kaneko Laboratory, Department of Cell Growth and Differentiation, Kyoto University, Shogoin-Kawahara-cho, Sakyo-ku, Kyoto, JapanTakeda-CiRA Joint Program (T-CiRA), Fujisawa, JapanIntroductionT cells induced from induced pluripotent stem cells(iPSCs) derived from antigen-specific T cells (T-iPS-T cells) are an attractive tool for T cell immunotherapy. The induction of cytotoxic T-iPS-T cells is well established in feeder-free condition for the aim of off-the-shelf production, however, the induction of helper T-iPS-T cells remains challenging.MethodsWe analyzed T-iPS-T cells matured in 3D organoid culture at different steps in the culture process at the single-cell level. T-iPS-T cell datasets were merged with an available human thymocyte dataset based in single-cell RNA sequencing (scRNA-seq). Particularly, we searched for genes crucial for generation CD4+ T-iPS-T cells by comparing T-iPS-T cells established in 2D feeder-free or 3D organoid culture.ResultsThe scRNA-seq data indicated that T-iPS-T cells are similar to T cells transitioning to human thymocytes, with SELENOW, GIMAP4, 7, SATB1, SALMF1, IL7R, SYTL2, S100A11, STAT1, IFITM1, LZTFL1 and SOX4 identified as candidate genes for the 2D feeder-free induction of CD4+ T-iPS-T cells.DiscussionThis study provides single cell transcriptome datasets of iPS-T cells and leads to further analysis for CD4+ T cell generation from T-iPSCs.https://www.frontiersin.org/articles/10.3389/fimmu.2023.1303713/fulliPSCT cell differentiationCD43D organoidscRNA seq |
| spellingShingle | Yoshitaka Ishiguro Yoshitaka Ishiguro Yoshitaka Ishiguro Shoichi Iriguchi Shoichi Iriguchi Shinya Asano Tokuyuki Shinohara Tokuyuki Shinohara Sara Shiina Sara Shiina Suguru Arima Suguru Arima Yoshiaki Kassai Yoshiaki Kassai Yoshiharu Sakai Kazutaka Obama Shin Kaneko Shin Kaneko Lineage tracing of T cell differentiation from T-iPSC by 2D feeder-free culture and 3D organoid culture Frontiers in Immunology iPSC T cell differentiation CD4 3D organoid scRNA seq |
| title | Lineage tracing of T cell differentiation from T-iPSC by 2D feeder-free culture and 3D organoid culture |
| title_full | Lineage tracing of T cell differentiation from T-iPSC by 2D feeder-free culture and 3D organoid culture |
| title_fullStr | Lineage tracing of T cell differentiation from T-iPSC by 2D feeder-free culture and 3D organoid culture |
| title_full_unstemmed | Lineage tracing of T cell differentiation from T-iPSC by 2D feeder-free culture and 3D organoid culture |
| title_short | Lineage tracing of T cell differentiation from T-iPSC by 2D feeder-free culture and 3D organoid culture |
| title_sort | lineage tracing of t cell differentiation from t ipsc by 2d feeder free culture and 3d organoid culture |
| topic | iPSC T cell differentiation CD4 3D organoid scRNA seq |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1303713/full |
| work_keys_str_mv | AT yoshitakaishiguro lineagetracingoftcelldifferentiationfromtipscby2dfeederfreecultureand3dorganoidculture AT yoshitakaishiguro lineagetracingoftcelldifferentiationfromtipscby2dfeederfreecultureand3dorganoidculture AT yoshitakaishiguro lineagetracingoftcelldifferentiationfromtipscby2dfeederfreecultureand3dorganoidculture AT shoichiiriguchi lineagetracingoftcelldifferentiationfromtipscby2dfeederfreecultureand3dorganoidculture AT shoichiiriguchi lineagetracingoftcelldifferentiationfromtipscby2dfeederfreecultureand3dorganoidculture AT shinyaasano lineagetracingoftcelldifferentiationfromtipscby2dfeederfreecultureand3dorganoidculture AT tokuyukishinohara lineagetracingoftcelldifferentiationfromtipscby2dfeederfreecultureand3dorganoidculture AT tokuyukishinohara lineagetracingoftcelldifferentiationfromtipscby2dfeederfreecultureand3dorganoidculture AT sarashiina lineagetracingoftcelldifferentiationfromtipscby2dfeederfreecultureand3dorganoidculture AT sarashiina lineagetracingoftcelldifferentiationfromtipscby2dfeederfreecultureand3dorganoidculture AT suguruarima lineagetracingoftcelldifferentiationfromtipscby2dfeederfreecultureand3dorganoidculture AT suguruarima lineagetracingoftcelldifferentiationfromtipscby2dfeederfreecultureand3dorganoidculture AT yoshiakikassai lineagetracingoftcelldifferentiationfromtipscby2dfeederfreecultureand3dorganoidculture AT yoshiakikassai lineagetracingoftcelldifferentiationfromtipscby2dfeederfreecultureand3dorganoidculture AT yoshiharusakai lineagetracingoftcelldifferentiationfromtipscby2dfeederfreecultureand3dorganoidculture AT kazutakaobama lineagetracingoftcelldifferentiationfromtipscby2dfeederfreecultureand3dorganoidculture AT shinkaneko lineagetracingoftcelldifferentiationfromtipscby2dfeederfreecultureand3dorganoidculture AT shinkaneko lineagetracingoftcelldifferentiationfromtipscby2dfeederfreecultureand3dorganoidculture |