Identification and Evaluation of Regulatory Role of miR164b in Malaysian Rice Variety (MR303) under Drought Stress
MR303 is a newly released Malaysian rice cultivar that comes with special traits as it can be cultivated in less fertile soils. Previous studies stated that miR164b is a key molecule that is responsible for regulating drought stress tolerance in many rice varieties. Thus, this study aims to identify...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
University of Brawijaya
2023-06-01
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| Series: | Journal of Tropical Life Science |
| Subjects: | |
| Online Access: | https://jtrolis.ub.ac.id/index.php/jtrolis/article/view/2612 |
| _version_ | 1797688456823963648 |
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| author | Catherine Han Yong Xin Chee Teng Chay Abdul Fatah |
| author_facet | Catherine Han Yong Xin Chee Teng Chay Abdul Fatah |
| author_sort | Catherine Han Yong Xin |
| collection | DOAJ |
| description | MR303 is a newly released Malaysian rice cultivar that comes with special traits as it can be cultivated in less fertile soils. Previous studies stated that miR164b is a key molecule that is responsible for regulating drought stress tolerance in many rice varieties. Thus, this study aims to identify the presence and regulatory role of miR164b in the MR303 variety using both computational and experimental approaches. The stem-loop structure of miR164b (pre-miR164b) was identified through reverse-transcriptase PCR (RT-PCR) with a size of ~ 100 bp. The target prediction by psRNA target revealed that the target gene of miR164b is the NAM protein of the NAC transcription factor. A gene expression study by RT-PCR followed by Image J analysis in both control and drought-treated plants demonstrated low expression of miR164b was observed in the drought sample, which led to the accumulation of its target, NAM1. This study provides preliminary knowledge of the presence of miR164b and its regulatory role in the MR303 rice variety. |
| first_indexed | 2024-03-12T01:31:15Z |
| format | Article |
| id | doaj.art-69600061dad745b68e51dc8e6bc077b8 |
| institution | Directory Open Access Journal |
| issn | 2087-5517 2527-4376 |
| language | English |
| last_indexed | 2024-03-12T01:31:15Z |
| publishDate | 2023-06-01 |
| publisher | University of Brawijaya |
| record_format | Article |
| series | Journal of Tropical Life Science |
| spelling | doaj.art-69600061dad745b68e51dc8e6bc077b82023-09-12T01:32:28ZengUniversity of BrawijayaJournal of Tropical Life Science2087-55172527-43762023-06-01132399 – 406399 – 40610.11594/jtls.13.02.192557Identification and Evaluation of Regulatory Role of miR164b in Malaysian Rice Variety (MR303) under Drought StressCatherine Han Yong XinChee Teng ChayAbdul FatahMR303 is a newly released Malaysian rice cultivar that comes with special traits as it can be cultivated in less fertile soils. Previous studies stated that miR164b is a key molecule that is responsible for regulating drought stress tolerance in many rice varieties. Thus, this study aims to identify the presence and regulatory role of miR164b in the MR303 variety using both computational and experimental approaches. The stem-loop structure of miR164b (pre-miR164b) was identified through reverse-transcriptase PCR (RT-PCR) with a size of ~ 100 bp. The target prediction by psRNA target revealed that the target gene of miR164b is the NAM protein of the NAC transcription factor. A gene expression study by RT-PCR followed by Image J analysis in both control and drought-treated plants demonstrated low expression of miR164b was observed in the drought sample, which led to the accumulation of its target, NAM1. This study provides preliminary knowledge of the presence of miR164b and its regulatory role in the MR303 rice variety.https://jtrolis.ub.ac.id/index.php/jtrolis/article/view/2612drought mir164, mr303nam 1 rice |
| spellingShingle | Catherine Han Yong Xin Chee Teng Chay Abdul Fatah Identification and Evaluation of Regulatory Role of miR164b in Malaysian Rice Variety (MR303) under Drought Stress Journal of Tropical Life Science drought mir164, mr303 nam 1 rice |
| title | Identification and Evaluation of Regulatory Role of miR164b in Malaysian Rice Variety (MR303) under Drought Stress |
| title_full | Identification and Evaluation of Regulatory Role of miR164b in Malaysian Rice Variety (MR303) under Drought Stress |
| title_fullStr | Identification and Evaluation of Regulatory Role of miR164b in Malaysian Rice Variety (MR303) under Drought Stress |
| title_full_unstemmed | Identification and Evaluation of Regulatory Role of miR164b in Malaysian Rice Variety (MR303) under Drought Stress |
| title_short | Identification and Evaluation of Regulatory Role of miR164b in Malaysian Rice Variety (MR303) under Drought Stress |
| title_sort | identification and evaluation of regulatory role of mir164b in malaysian rice variety mr303 under drought stress |
| topic | drought mir164, mr303 nam 1 rice |
| url | https://jtrolis.ub.ac.id/index.php/jtrolis/article/view/2612 |
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