Chicken Feather Keratin Peptides for the Control of Keratinocyte Migration

FAO estimates that in 2030 the poultry meat production could reach 120 million tons, which is a challenge in terms of waste management. Feathers are mainly composed of keratin, an important biomaterial. Using feathers as a source of keratin will minimize the waste generated, while contributing to su...

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Main Authors: Cláudia M. Botelho, Pedro Ferreira-Santos, Duarte Toubarro, Hugo Dinis, Hugo Osório, Augusto Costa-Barbosa, Paula Sampaio, Nelson Simões, José A. Teixeira
Format: Article
Language:English
Published: MDPI AG 2021-07-01
Series:Applied Sciences
Subjects:
Online Access:https://www.mdpi.com/2076-3417/11/15/6779
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author Cláudia M. Botelho
Pedro Ferreira-Santos
Duarte Toubarro
Hugo Dinis
Hugo Osório
Augusto Costa-Barbosa
Paula Sampaio
Nelson Simões
José A. Teixeira
author_facet Cláudia M. Botelho
Pedro Ferreira-Santos
Duarte Toubarro
Hugo Dinis
Hugo Osório
Augusto Costa-Barbosa
Paula Sampaio
Nelson Simões
José A. Teixeira
author_sort Cláudia M. Botelho
collection DOAJ
description FAO estimates that in 2030 the poultry meat production could reach 120 million tons, which is a challenge in terms of waste management. Feathers are mainly composed of keratin, an important biomaterial. Using feathers as a source of keratin will minimize the waste generated, while contributing to supply an important material for several industries, such as pharmaceutical and biomedical. The peptides were extracted from the feathers by microbial degradation. In this study, we evaluated the peptides effect on keratinocyte metabolic activity and migration. The influence of these peptides on non-activated and activated macrophages was also assessed. It was demonstrated that depending on the keratin peptide fraction in contact with keratinocytes, it is possible to modulate the migration rate of the keratinocytes. Peptide fraction with low molecular weight increases migration, while peptides with a high range of molecular sizes decreases it. Some peptide fractions induce the secretion of TNF-α in non-activated macrophages and not on activated macrophages, demonstrating that these peptides should only be placed in contact with cells, in the context of an ongoing inflammatory process. This work is a step forward on the understanding of keratin peptides influence on keratinocytes and immune cells system cells, macrophages.
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spelling doaj.art-696326ea11fb4d3492f37a57b88ff5982023-11-22T05:19:37ZengMDPI AGApplied Sciences2076-34172021-07-011115677910.3390/app11156779Chicken Feather Keratin Peptides for the Control of Keratinocyte MigrationCláudia M. Botelho0Pedro Ferreira-Santos1Duarte Toubarro2Hugo Dinis3Hugo Osório4Augusto Costa-Barbosa5Paula Sampaio6Nelson Simões7José A. Teixeira8Centre of Biological Engineering, Universidade do Minho, 4710-057 Braga, PortugalCentre of Biological Engineering, Universidade do Minho, 4710-057 Braga, PortugalCBA—Biotechnology Centre of Azores, Faculty of Sciences and Technology, University of Azores, 9500-321 Ponta Delgada, Açores, PortugalCentre of Biological Engineering, Universidade do Minho, 4710-057 Braga, Portugali3S—Instituto de Investigação e Inovação em Saúde, Universidade do Porto, 4200-135 Porto, PortugalCBMA—Centre of Molecular and Environmental Biology, Department of Biology, University of Minho, 4710-057 Braga, PortugalCBMA—Centre of Molecular and Environmental Biology, Department of Biology, University of Minho, 4710-057 Braga, PortugalCBA—Biotechnology Centre of Azores, Faculty of Sciences and Technology, University of Azores, 9500-321 Ponta Delgada, Açores, PortugalCentre of Biological Engineering, Universidade do Minho, 4710-057 Braga, PortugalFAO estimates that in 2030 the poultry meat production could reach 120 million tons, which is a challenge in terms of waste management. Feathers are mainly composed of keratin, an important biomaterial. Using feathers as a source of keratin will minimize the waste generated, while contributing to supply an important material for several industries, such as pharmaceutical and biomedical. The peptides were extracted from the feathers by microbial degradation. In this study, we evaluated the peptides effect on keratinocyte metabolic activity and migration. The influence of these peptides on non-activated and activated macrophages was also assessed. It was demonstrated that depending on the keratin peptide fraction in contact with keratinocytes, it is possible to modulate the migration rate of the keratinocytes. Peptide fraction with low molecular weight increases migration, while peptides with a high range of molecular sizes decreases it. Some peptide fractions induce the secretion of TNF-α in non-activated macrophages and not on activated macrophages, demonstrating that these peptides should only be placed in contact with cells, in the context of an ongoing inflammatory process. This work is a step forward on the understanding of keratin peptides influence on keratinocytes and immune cells system cells, macrophages.https://www.mdpi.com/2076-3417/11/15/6779chicken feathersmicrobial hydrolysiskeratin peptideskeratinocytescellular migrationinflammation
spellingShingle Cláudia M. Botelho
Pedro Ferreira-Santos
Duarte Toubarro
Hugo Dinis
Hugo Osório
Augusto Costa-Barbosa
Paula Sampaio
Nelson Simões
José A. Teixeira
Chicken Feather Keratin Peptides for the Control of Keratinocyte Migration
Applied Sciences
chicken feathers
microbial hydrolysis
keratin peptides
keratinocytes
cellular migration
inflammation
title Chicken Feather Keratin Peptides for the Control of Keratinocyte Migration
title_full Chicken Feather Keratin Peptides for the Control of Keratinocyte Migration
title_fullStr Chicken Feather Keratin Peptides for the Control of Keratinocyte Migration
title_full_unstemmed Chicken Feather Keratin Peptides for the Control of Keratinocyte Migration
title_short Chicken Feather Keratin Peptides for the Control of Keratinocyte Migration
title_sort chicken feather keratin peptides for the control of keratinocyte migration
topic chicken feathers
microbial hydrolysis
keratin peptides
keratinocytes
cellular migration
inflammation
url https://www.mdpi.com/2076-3417/11/15/6779
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