Untargeted metabolomics approach for unraveling robust biomarkers of nutritional status in fasted gilthead sea bream (Sparus aurata)
A metabolomic study has been performed to identify sensitive and robust biomarkers of malnutrition in farmed fish, using gilthead sea bream (Sparus aurata) as a model. The metabolomic fingerprinting of serum from fasted fish was assessed by means of ultra-high performance liquid chromatography coupl...
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PeerJ Inc.
2017-01-01
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| Online Access: | https://peerj.com/articles/2920.pdf |
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| author | Ruben Gil-Solsona Jaime Nácher-Mestre Leticia Lacalle-Bergeron Juan Vicente Sancho Josep Alvar Calduch-Giner Félix Hernández Jaume Pérez-Sánchez |
| author_facet | Ruben Gil-Solsona Jaime Nácher-Mestre Leticia Lacalle-Bergeron Juan Vicente Sancho Josep Alvar Calduch-Giner Félix Hernández Jaume Pérez-Sánchez |
| author_sort | Ruben Gil-Solsona |
| collection | DOAJ |
| description | A metabolomic study has been performed to identify sensitive and robust biomarkers of malnutrition in farmed fish, using gilthead sea bream (Sparus aurata) as a model. The metabolomic fingerprinting of serum from fasted fish was assessed by means of ultra-high performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry. More than 15,000 different m/z ions were detected and Partial Least Squares–Discriminant analysis allowed a clear differentiation between the two experimental groups (fed and 10-day fasted fish) with more than 90% of total variance explained by the two first components. The most significant metabolites (up to 45) were elucidated on the basis of their tandem mass spectra with a broad representation of amino acids, oligopeptides, urea cycle metabolites, L-carnitine-related metabolites, glutathione-related metabolites, fatty acids, lysophosphatidic acids, phosphatidylcholines as well as biotin- and noradrenaline-related metabolites. This untargeted approach highlighted important adaptive responses in energy and oxidative metabolism, contributing to identify robust and nutritionally-regulated biomarkers of health and metabolic condition that will serve to assess the welfare status of farmed fish. |
| first_indexed | 2024-03-09T06:33:00Z |
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| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:33:00Z |
| publishDate | 2017-01-01 |
| publisher | PeerJ Inc. |
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| spelling | doaj.art-696508776d09417fb8cafaa7b0ad87652023-12-03T11:03:12ZengPeerJ Inc.PeerJ2167-83592017-01-015e292010.7717/peerj.2920Untargeted metabolomics approach for unraveling robust biomarkers of nutritional status in fasted gilthead sea bream (Sparus aurata)Ruben Gil-Solsona0Jaime Nácher-Mestre1Leticia Lacalle-Bergeron2Juan Vicente Sancho3Josep Alvar Calduch-Giner4Félix Hernández5Jaume Pérez-Sánchez6Research Institute for Pesticides and Water (IUPA), University Jaume I, Castellón, SpainResearch Institute for Pesticides and Water (IUPA), University Jaume I, Castellón, SpainResearch Institute for Pesticides and Water (IUPA), University Jaume I, Castellón, SpainResearch Institute for Pesticides and Water (IUPA), University Jaume I, Castellón, SpainInstitute of Aquaculture Torre de la Sal (IATS, CSIC), Ribera de Cabanes, Castellón, SpainResearch Institute for Pesticides and Water (IUPA), University Jaume I, Castellón, SpainInstitute of Aquaculture Torre de la Sal (IATS, CSIC), Ribera de Cabanes, Castellón, SpainA metabolomic study has been performed to identify sensitive and robust biomarkers of malnutrition in farmed fish, using gilthead sea bream (Sparus aurata) as a model. The metabolomic fingerprinting of serum from fasted fish was assessed by means of ultra-high performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry. More than 15,000 different m/z ions were detected and Partial Least Squares–Discriminant analysis allowed a clear differentiation between the two experimental groups (fed and 10-day fasted fish) with more than 90% of total variance explained by the two first components. The most significant metabolites (up to 45) were elucidated on the basis of their tandem mass spectra with a broad representation of amino acids, oligopeptides, urea cycle metabolites, L-carnitine-related metabolites, glutathione-related metabolites, fatty acids, lysophosphatidic acids, phosphatidylcholines as well as biotin- and noradrenaline-related metabolites. This untargeted approach highlighted important adaptive responses in energy and oxidative metabolism, contributing to identify robust and nutritionally-regulated biomarkers of health and metabolic condition that will serve to assess the welfare status of farmed fish.https://peerj.com/articles/2920.pdfAquacultureNutritionChromatographyMass spectrometryGilthead sea breamSerum metabolomics |
| spellingShingle | Ruben Gil-Solsona Jaime Nácher-Mestre Leticia Lacalle-Bergeron Juan Vicente Sancho Josep Alvar Calduch-Giner Félix Hernández Jaume Pérez-Sánchez Untargeted metabolomics approach for unraveling robust biomarkers of nutritional status in fasted gilthead sea bream (Sparus aurata) PeerJ Aquaculture Nutrition Chromatography Mass spectrometry Gilthead sea bream Serum metabolomics |
| title | Untargeted metabolomics approach for unraveling robust biomarkers of nutritional status in fasted gilthead sea bream (Sparus aurata) |
| title_full | Untargeted metabolomics approach for unraveling robust biomarkers of nutritional status in fasted gilthead sea bream (Sparus aurata) |
| title_fullStr | Untargeted metabolomics approach for unraveling robust biomarkers of nutritional status in fasted gilthead sea bream (Sparus aurata) |
| title_full_unstemmed | Untargeted metabolomics approach for unraveling robust biomarkers of nutritional status in fasted gilthead sea bream (Sparus aurata) |
| title_short | Untargeted metabolomics approach for unraveling robust biomarkers of nutritional status in fasted gilthead sea bream (Sparus aurata) |
| title_sort | untargeted metabolomics approach for unraveling robust biomarkers of nutritional status in fasted gilthead sea bream sparus aurata |
| topic | Aquaculture Nutrition Chromatography Mass spectrometry Gilthead sea bream Serum metabolomics |
| url | https://peerj.com/articles/2920.pdf |
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