SNP-based bulk segregant analysis revealed disease resistance QTLs associated with northern corn leaf blight in maize
Maize (Zea mays L.) is the most important food security crop worldwide. Northern corn leaf blight (NCLB), caused by Exserohilum turcicum, severely reduces production causing millions of dollars in losses worldwide. Therefore, this study aimed to identify significant QTLs associated with NCLB by util...
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2022.1038948/full |
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| author | Ruining Zhai Aihua Huang Runxiu Mo Chenglin Zou Xinxing Wei Meng Yang Hua Tan Kaijian Huang Jie Qin |
| author_facet | Ruining Zhai Aihua Huang Runxiu Mo Chenglin Zou Xinxing Wei Meng Yang Hua Tan Kaijian Huang Jie Qin |
| author_sort | Ruining Zhai |
| collection | DOAJ |
| description | Maize (Zea mays L.) is the most important food security crop worldwide. Northern corn leaf blight (NCLB), caused by Exserohilum turcicum, severely reduces production causing millions of dollars in losses worldwide. Therefore, this study aimed to identify significant QTLs associated with NCLB by utilizing next-generation sequencing-based bulked-segregant analysis (BSA). Parental lines GML71 (resistant) and Gui A10341 (susceptible) were used to develop segregating population F2. Two bulks with 30 plants each were further selected from the segregating population for sequencing along with the parental lines. High throughput sequencing data was used for BSA. We identified 10 QTLs on Chr 1, Chr 2, Chr 3, and Chr 5 with 265 non-synonymous SNPs. Moreover, based on annotation information, we identified 27 candidate genes in the QTL regions. The candidate genes associated with disease resistance include AATP1, At4g24790, STICHEL-like 2, BI O 3-BIO1, ZAR1, SECA2, ABCG25, LECRK54, MKK7, MKK9, RLK902, and DEAD-box ATP-dependent RNA helicase. The annotation information suggested their involvement in disease resistance-related pathways, including protein phosphorylation, cytoplasmic vesicle, protein serine/threonine kinase activity, and ATP binding pathways. Our study provides a substantial addition to the available information regarding QTLs associated with NCLB, and further functional verification of identified candidate genes can broaden the scope of understanding the NCLB resistance mechanism in maize. |
| first_indexed | 2024-04-12T06:43:42Z |
| format | Article |
| id | doaj.art-6966e5f20fbf4661aa558935ad43e23c |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-04-12T06:43:42Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-6966e5f20fbf4661aa558935ad43e23c2022-12-22T03:43:39ZengFrontiers Media S.A.Frontiers in Genetics1664-80212022-11-011310.3389/fgene.2022.10389481038948SNP-based bulk segregant analysis revealed disease resistance QTLs associated with northern corn leaf blight in maizeRuining Zhai0Aihua Huang1Runxiu Mo2Chenglin Zou3Xinxing Wei4Meng Yang5Hua Tan6Kaijian Huang7Jie Qin8Maize Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi, ChinaMaize Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi, ChinaMaize Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi, ChinaMaize Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi, ChinaMaize Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi, ChinaMaize Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi, ChinaMaize Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi, ChinaMaize Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi, ChinaGuangxi Academy of Agricultural Sciences, Nanning, Guangxi, ChinaMaize (Zea mays L.) is the most important food security crop worldwide. Northern corn leaf blight (NCLB), caused by Exserohilum turcicum, severely reduces production causing millions of dollars in losses worldwide. Therefore, this study aimed to identify significant QTLs associated with NCLB by utilizing next-generation sequencing-based bulked-segregant analysis (BSA). Parental lines GML71 (resistant) and Gui A10341 (susceptible) were used to develop segregating population F2. Two bulks with 30 plants each were further selected from the segregating population for sequencing along with the parental lines. High throughput sequencing data was used for BSA. We identified 10 QTLs on Chr 1, Chr 2, Chr 3, and Chr 5 with 265 non-synonymous SNPs. Moreover, based on annotation information, we identified 27 candidate genes in the QTL regions. The candidate genes associated with disease resistance include AATP1, At4g24790, STICHEL-like 2, BI O 3-BIO1, ZAR1, SECA2, ABCG25, LECRK54, MKK7, MKK9, RLK902, and DEAD-box ATP-dependent RNA helicase. The annotation information suggested their involvement in disease resistance-related pathways, including protein phosphorylation, cytoplasmic vesicle, protein serine/threonine kinase activity, and ATP binding pathways. Our study provides a substantial addition to the available information regarding QTLs associated with NCLB, and further functional verification of identified candidate genes can broaden the scope of understanding the NCLB resistance mechanism in maize.https://www.frontiersin.org/articles/10.3389/fgene.2022.1038948/fullnorthern corn leaf blightdisease resistancebulk segregant analysisSNPQTL |
| spellingShingle | Ruining Zhai Aihua Huang Runxiu Mo Chenglin Zou Xinxing Wei Meng Yang Hua Tan Kaijian Huang Jie Qin SNP-based bulk segregant analysis revealed disease resistance QTLs associated with northern corn leaf blight in maize Frontiers in Genetics northern corn leaf blight disease resistance bulk segregant analysis SNP QTL |
| title | SNP-based bulk segregant analysis revealed disease resistance QTLs associated with northern corn leaf blight in maize |
| title_full | SNP-based bulk segregant analysis revealed disease resistance QTLs associated with northern corn leaf blight in maize |
| title_fullStr | SNP-based bulk segregant analysis revealed disease resistance QTLs associated with northern corn leaf blight in maize |
| title_full_unstemmed | SNP-based bulk segregant analysis revealed disease resistance QTLs associated with northern corn leaf blight in maize |
| title_short | SNP-based bulk segregant analysis revealed disease resistance QTLs associated with northern corn leaf blight in maize |
| title_sort | snp based bulk segregant analysis revealed disease resistance qtls associated with northern corn leaf blight in maize |
| topic | northern corn leaf blight disease resistance bulk segregant analysis SNP QTL |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2022.1038948/full |
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