Motility in <i>Periweissella</i> Species: Genomic and Phenotypic Characterization and Update on Motility in <i>Lactobacillaceae</i>
The genus <i>Weissella</i> and the recently described genus <i>Periweissella</i>, to which some previously named <i>Weissella</i> species have been reclassified as a result of a taxogenomic assessment, includes lactic acid bacteria species with high biotechnologic...
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MDPI AG
2023-12-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/12/2923 |
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| author | Francesca Fanelli Marco Montemurro Daniele Chieffi Gyu-Sung Cho Hui-Zhi Low Frank Hille Charles M. A. P. Franz Vincenzina Fusco |
| author_facet | Francesca Fanelli Marco Montemurro Daniele Chieffi Gyu-Sung Cho Hui-Zhi Low Frank Hille Charles M. A. P. Franz Vincenzina Fusco |
| author_sort | Francesca Fanelli |
| collection | DOAJ |
| description | The genus <i>Weissella</i> and the recently described genus <i>Periweissella</i>, to which some previously named <i>Weissella</i> species have been reclassified as a result of a taxogenomic assessment, includes lactic acid bacteria species with high biotechnological and probiotic potential. Only one species, namely, <i>Periweissella</i> (<i>P</i>.) <i>beninensis</i>, whose type strain has been shown to possess probiotic features, has so far been described to be motile. However, the availability of numerous genome sequences of <i>Weissella</i> and <i>Periweissella</i> species prompted the possibility to screen for the presence of the genetic determinants encoding motility in <i>Weissella</i> and <i>Periweissellas</i> spp. other than <i>P. beninensis</i>. Herein, we performed a comprehensive genomic analysis to identify motility-related proteins in all <i>Weissella</i> and <i>Periweissella</i> species described so far, and extended the analysis to the recently sequenced <i>Lactobacillaceae</i> spp. Furthermore, we performed motility assays and transmission electron microscopy (TEM) on <i>Periweissella</i> type strains to confirm the genomic prediction. The homology-based analysis revealed genes coding for motility proteins only in the type strains of <i>P. beninensis</i>, <i>P. fabalis</i>, <i>P. fabaria</i> and <i>P. ghanensis</i> genomes. However, only the <i>P. beninensis</i> type strain was positive in the motility assay and displayed run-and-tumble behavior. Many peritrichous and long flagella on bacterial cells were visualized via TEM, as well. As for the <i>Lactobacillaceae</i>, in addition to the species previously described to harbor motility proteins, the genetic determinants of motility were also found in the genomes of the type strains of <i>Lactobacillus rogosae</i> and <i>Ligilactobacillus salitolerans</i>. This study, which is one of the first to analyze the genomes of <i>Weissella</i>, <i>Periweissella</i> and the recently sequenced <i>Lactobacillaceae</i> spp. for the presence of genes coding for motility proteins and which assesses the associated motility phenotypes, provides novel results that expand knowledge on these genera and are useful in the further characterization of lactic acid bacteria. |
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| last_indexed | 2024-03-08T20:30:54Z |
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| spelling | doaj.art-6966efd12280443c8da5329102450b5b2023-12-22T14:26:05ZengMDPI AGMicroorganisms2076-26072023-12-011112292310.3390/microorganisms11122923Motility in <i>Periweissella</i> Species: Genomic and Phenotypic Characterization and Update on Motility in <i>Lactobacillaceae</i>Francesca Fanelli0Marco Montemurro1Daniele Chieffi2Gyu-Sung Cho3Hui-Zhi Low4Frank Hille5Charles M. A. P. Franz6Vincenzina Fusco7National Research Council of Italy, Institute of Sciences of Food Production (CNR-ISPA), 70126 Bari, ItalyNational Research Council of Italy, Institute of Sciences of Food Production (CNR-ISPA), 70126 Bari, ItalyNational Research Council of Italy, Institute of Sciences of Food Production (CNR-ISPA), 70126 Bari, ItalyDepartment of Microbiology and Biotechnology, Max Rubner-Institut, 24103 Kiel, GermanyDepartment of Microbiology and Biotechnology, Max Rubner-Institut, 24103 Kiel, GermanyDepartment of Microbiology and Biotechnology, Max Rubner-Institut, 24103 Kiel, GermanyDepartment of Microbiology and Biotechnology, Max Rubner-Institut, 24103 Kiel, GermanyNational Research Council of Italy, Institute of Sciences of Food Production (CNR-ISPA), 70126 Bari, ItalyThe genus <i>Weissella</i> and the recently described genus <i>Periweissella</i>, to which some previously named <i>Weissella</i> species have been reclassified as a result of a taxogenomic assessment, includes lactic acid bacteria species with high biotechnological and probiotic potential. Only one species, namely, <i>Periweissella</i> (<i>P</i>.) <i>beninensis</i>, whose type strain has been shown to possess probiotic features, has so far been described to be motile. However, the availability of numerous genome sequences of <i>Weissella</i> and <i>Periweissella</i> species prompted the possibility to screen for the presence of the genetic determinants encoding motility in <i>Weissella</i> and <i>Periweissellas</i> spp. other than <i>P. beninensis</i>. Herein, we performed a comprehensive genomic analysis to identify motility-related proteins in all <i>Weissella</i> and <i>Periweissella</i> species described so far, and extended the analysis to the recently sequenced <i>Lactobacillaceae</i> spp. Furthermore, we performed motility assays and transmission electron microscopy (TEM) on <i>Periweissella</i> type strains to confirm the genomic prediction. The homology-based analysis revealed genes coding for motility proteins only in the type strains of <i>P. beninensis</i>, <i>P. fabalis</i>, <i>P. fabaria</i> and <i>P. ghanensis</i> genomes. However, only the <i>P. beninensis</i> type strain was positive in the motility assay and displayed run-and-tumble behavior. Many peritrichous and long flagella on bacterial cells were visualized via TEM, as well. As for the <i>Lactobacillaceae</i>, in addition to the species previously described to harbor motility proteins, the genetic determinants of motility were also found in the genomes of the type strains of <i>Lactobacillus rogosae</i> and <i>Ligilactobacillus salitolerans</i>. This study, which is one of the first to analyze the genomes of <i>Weissella</i>, <i>Periweissella</i> and the recently sequenced <i>Lactobacillaceae</i> spp. for the presence of genes coding for motility proteins and which assesses the associated motility phenotypes, provides novel results that expand knowledge on these genera and are useful in the further characterization of lactic acid bacteria.https://www.mdpi.com/2076-2607/11/12/2923motilityflagella<i>fliH</i><i>fliN</i><i>Periweissella</i>transmission electron microscopy |
| spellingShingle | Francesca Fanelli Marco Montemurro Daniele Chieffi Gyu-Sung Cho Hui-Zhi Low Frank Hille Charles M. A. P. Franz Vincenzina Fusco Motility in <i>Periweissella</i> Species: Genomic and Phenotypic Characterization and Update on Motility in <i>Lactobacillaceae</i> Microorganisms motility flagella <i>fliH</i> <i>fliN</i> <i>Periweissella</i> transmission electron microscopy |
| title | Motility in <i>Periweissella</i> Species: Genomic and Phenotypic Characterization and Update on Motility in <i>Lactobacillaceae</i> |
| title_full | Motility in <i>Periweissella</i> Species: Genomic and Phenotypic Characterization and Update on Motility in <i>Lactobacillaceae</i> |
| title_fullStr | Motility in <i>Periweissella</i> Species: Genomic and Phenotypic Characterization and Update on Motility in <i>Lactobacillaceae</i> |
| title_full_unstemmed | Motility in <i>Periweissella</i> Species: Genomic and Phenotypic Characterization and Update on Motility in <i>Lactobacillaceae</i> |
| title_short | Motility in <i>Periweissella</i> Species: Genomic and Phenotypic Characterization and Update on Motility in <i>Lactobacillaceae</i> |
| title_sort | motility in i periweissella i species genomic and phenotypic characterization and update on motility in i lactobacillaceae i |
| topic | motility flagella <i>fliH</i> <i>fliN</i> <i>Periweissella</i> transmission electron microscopy |
| url | https://www.mdpi.com/2076-2607/11/12/2923 |
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