Three-Dimensional Reservoir-Based Dielectrophoresis (rDEP) for Enhanced Particle Enrichment
Selective enrichment of target species is crucial for a wide variety of engineering systems for improved performance of subsequent processes. Dielectrophoresis (DEP) is a powerful electrokinetic method that can be used to focus, trap, concentrate, and separate a variety of species in a label-free ma...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2018-03-01
|
| Series: | Micromachines |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2072-666X/9/3/123 |
| _version_ | 1811341165142736896 |
|---|---|
| author | Akshay Kale Saurin Patel Xiangchun Xuan |
| author_facet | Akshay Kale Saurin Patel Xiangchun Xuan |
| author_sort | Akshay Kale |
| collection | DOAJ |
| description | Selective enrichment of target species is crucial for a wide variety of engineering systems for improved performance of subsequent processes. Dielectrophoresis (DEP) is a powerful electrokinetic method that can be used to focus, trap, concentrate, and separate a variety of species in a label-free manner. The commonly employed methods for DEP suffer from limitations such as electrode fouling and high susceptibility to Joule heating effects. Recently, our group has demonstrated DEP-based manipulations of particles and cells using a novel method of reservoir-based dielectrophoresis (rDEP) which exploits the naturally produced electric field gradients at the reservoir-microchannel junction. Although this method reasonably addresses the limitations mentioned above while maintaining a high simplicity of fabrication, all of our demonstrations so far have used a two-dimensional rDEP, which limits the performance of the devices. This work aims to improve their performance further by making the DEP three-dimensional. Through detailed experimental and numerical analysis, we demonstrate a six-fold increase in the enrichment performance of latex beads and a significant reduction in the power consumption for the new devices, which would allow a more reliable integration of the same into micro-total analysis systems. |
| first_indexed | 2024-04-13T18:52:48Z |
| format | Article |
| id | doaj.art-696b9b12cab8425d912971a54dd75b88 |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-04-13T18:52:48Z |
| publishDate | 2018-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-696b9b12cab8425d912971a54dd75b882022-12-22T02:34:22ZengMDPI AGMicromachines2072-666X2018-03-019312310.3390/mi9030123mi9030123Three-Dimensional Reservoir-Based Dielectrophoresis (rDEP) for Enhanced Particle EnrichmentAkshay Kale0Saurin Patel1Xiangchun Xuan2Department of Mechanical Engineering, Clemson University, Clemson, SC 29634-0921, USADepartment of Mechanical Engineering, Clemson University, Clemson, SC 29634-0921, USADepartment of Mechanical Engineering, Clemson University, Clemson, SC 29634-0921, USASelective enrichment of target species is crucial for a wide variety of engineering systems for improved performance of subsequent processes. Dielectrophoresis (DEP) is a powerful electrokinetic method that can be used to focus, trap, concentrate, and separate a variety of species in a label-free manner. The commonly employed methods for DEP suffer from limitations such as electrode fouling and high susceptibility to Joule heating effects. Recently, our group has demonstrated DEP-based manipulations of particles and cells using a novel method of reservoir-based dielectrophoresis (rDEP) which exploits the naturally produced electric field gradients at the reservoir-microchannel junction. Although this method reasonably addresses the limitations mentioned above while maintaining a high simplicity of fabrication, all of our demonstrations so far have used a two-dimensional rDEP, which limits the performance of the devices. This work aims to improve their performance further by making the DEP three-dimensional. Through detailed experimental and numerical analysis, we demonstrate a six-fold increase in the enrichment performance of latex beads and a significant reduction in the power consumption for the new devices, which would allow a more reliable integration of the same into micro-total analysis systems.http://www.mdpi.com/2072-666X/9/3/123dielectrophoresiselectrokineticsparticle focusingparticle trappingmicrofluidics |
| spellingShingle | Akshay Kale Saurin Patel Xiangchun Xuan Three-Dimensional Reservoir-Based Dielectrophoresis (rDEP) for Enhanced Particle Enrichment Micromachines dielectrophoresis electrokinetics particle focusing particle trapping microfluidics |
| title | Three-Dimensional Reservoir-Based Dielectrophoresis (rDEP) for Enhanced Particle Enrichment |
| title_full | Three-Dimensional Reservoir-Based Dielectrophoresis (rDEP) for Enhanced Particle Enrichment |
| title_fullStr | Three-Dimensional Reservoir-Based Dielectrophoresis (rDEP) for Enhanced Particle Enrichment |
| title_full_unstemmed | Three-Dimensional Reservoir-Based Dielectrophoresis (rDEP) for Enhanced Particle Enrichment |
| title_short | Three-Dimensional Reservoir-Based Dielectrophoresis (rDEP) for Enhanced Particle Enrichment |
| title_sort | three dimensional reservoir based dielectrophoresis rdep for enhanced particle enrichment |
| topic | dielectrophoresis electrokinetics particle focusing particle trapping microfluidics |
| url | http://www.mdpi.com/2072-666X/9/3/123 |
| work_keys_str_mv | AT akshaykale threedimensionalreservoirbaseddielectrophoresisrdepforenhancedparticleenrichment AT saurinpatel threedimensionalreservoirbaseddielectrophoresisrdepforenhancedparticleenrichment AT xiangchunxuan threedimensionalreservoirbaseddielectrophoresisrdepforenhancedparticleenrichment |